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[178023_s_at]

Description K09E4.3 /REP_DB=WormBase Gene ID /WP=CE19996 /TR=Q9NAP7 /GB=CAB70169.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 79      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 84      6grp_total > 79      4grp_total > 58      6grp_highest_sub > Cu      6grp_high1_sub > Cu      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 518
1 2 3 4 range
A 659.5
(-0)
P 		895.7
(3)
P 		872.7
(2.7)
P 		425
(-3)
P 		471
B 634.5
(-0.3)
P 		846.9
(2.2)
P 		915.8
(3)
P 		398.2
(-3)
P 		518
C 579.5
(-1.4)
P 		869.1
(3)
P 		806.8
(2)
P 		478.5
(-3)
P 		391
avr 624.5
(-0.4) 		870.6
(3) 		865.1
(2.9) 		433.9
(-3) 		437

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 405
1 2 3 4 5 6 range
A 845.3
(3)
P 		511.6
(-3)
P 		624.6
(-0.9)
P 		509
(-3)
P 		651.9
(-0.5)
P 		644.7
(-0.6)
P 		336
B 638.8
(-0.8)
P 		592.3
(-1.9)
P 		660.1
(-0.3)
P 		545.5
(-3)
P 		795.8
(3)
P 		738.3
(1.6)
P 		250
C 440.8
(-3)
P 		696.9
(1.2)
P 		600.8
(-0.3)
P 		460.9
(-2.7)
P 		560.5
(-1)
P 		802.9
(3)
P 		362
avr 641.6
(0.7) 		600.3
(-0.4) 		628.5
(0.3) 		505.1
(-3) 		669.4
(1.4) 		728.6
(3) 		224
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 219
1 2 3 4 range
A 240.1
(3)
P 		194.4
(1.5)
P 		228.1
(2.6)
P 		62.2
(-3)
P 		178
B 202.3
(2.5)
P 		209.2
(2.9)
P 		212.2
(3)
P 		84.9
(-3)
P 		127
C 191.8
(0.3)
P 		249.9
(2.1)
P 		281.2
(3)
P 		80.7
(-3)
P 		201
avr 211.4
(1.9) 		217.8
(2.2) 		240.5
(3) 		75.9
(-3) 		165

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 231
1 2 3 4 5 6 range
A 155.8
(-0.6)
P 		182
(1.7)
P 		136
(-2.4)
P 		129.1
(-3)
P 		196.7
(3)
P 		157
(-0.5)
P 		68
B 222.3
(-0.4)
P 		197.1
(-1.2)
P 		160.3
(-2.5)
P 		144.5
(-3)
P 		323.3
(3)
P 		275.3
(1.4)
P 		179
C 163.4
(-0.6)
P 		212.6
(1.1)
P 		152.9
(-0.9)
P 		92.7
(-3)
P 		181.4
(0)
P 		268.8
(3)
P 		176
avr 180.5
(0.1) 		197.2
(1) 		149.7
(-1.5) 		122.1
(-3) 		233.8
(3) 		233.7
(3) 		112
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178023_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4014
Sequence Type Exemplar sequence
Representative Public ID K09E4.3 NCBI
Target Description K09E4.3 /REP_DB=WormBase Gene ID /WP=CE19996 /TR=Q9NAP7 /GB=CAB70169.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064548(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064548 NCBI Caenorhabditis elegans K09E4.3 (K09E4.3) mRNA, complete cds. 11/11 None
GENEFINDER00000022872 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14123942:14126831:-1 11/11 None
K09E4.3 ENSEMBL cdna:known chromosome:CEL140:II:14123857:14126832:-1 gene:K09E4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14123938-14126741(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K09E4.3
Entrez Gene 175064 Entrez gene
SwissProt Q9NAP7 EMBL-EBI
WormBase CE19996 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264198_AT expressed protein at
ATGENOME1:14569_AT expressed protein at
CANINE:1600303_AT similar to CG8067-PA cfa
CANINE:1587719_AT similar to CG8067-PA cfa
CANINE:1585322_X_AT similar to CG8067-PA cfa
CANINE:1585321_S_AT similar to CG8067-PA cfa
CANINE:1585320_AT similar to CG8067-PA cfa
CANINE_2:CFA.5501.1.A1_S_AT similar to CG8067-PA cfa
CANINE_2:CFAAFFX.9300.1.S1_AT similar to CG8067-PA cfa
CANINE_2:CFAAFFX.9303.1.S1_AT similar to CG8067-PA cfa
DROSOPHILA_2:1623302_AT dm
DROSGENOME1:155137_AT dm
CHICKEN:GGA.4347.1.S1_AT similar to chromosome 20 open reading frame 7 isoform 1 gga
HG-U133_PLUS_2:219524_S_AT chromosome 20 open reading frame 7 hs
HG-U133A:219524_S_AT chromosome 20 open reading frame 7 hs
HG-U133A_2:219524_S_AT chromosome 20 open reading frame 7 hs
HG-U133_PLUS_2:222894_X_AT chromosome 20 open reading frame 7 hs
HG-U133B:222894_X_AT chromosome 20 open reading frame 7 hs
HU35KSUBC:RC_N32022_AT chromosome 20 open reading frame 7 hs
HU35KSUBD:RC_AA610002_AT chromosome 20 open reading frame 7 hs
HG-U133_PLUS_2:227160_S_AT chromosome 20 open reading frame 7 hs
HG-U133B:227160_S_AT chromosome 20 open reading frame 7 hs
U133_X3P:G13129143_3P_S_AT chromosome 20 open reading frame 7 hs
U133_X3P:HS.44296.1.S1_3P_AT chromosome 20 open reading frame 7 hs
U133_X3P:HS.44296.0.S1_3P_S_AT chromosome 20 open reading frame 7 hs
U133_X3P:227160_3P_S_AT chromosome 20 open reading frame 7 hs
HG-U95C:62140_AT chromosome 20 open reading frame 7 hs
HG-U95C:62141_G_AT chromosome 20 open reading frame 7 hs
HG-U95C:62142_I_AT chromosome 20 open reading frame 7 hs
HG-U95C:62143_R_AT chromosome 20 open reading frame 7 hs
U133_X3P:HS.322904.0.S1_3P_AT Chromosome 20 open reading frame 7 hs
MOUSE430_2:1428745_A_AT RIKEN cDNA 2310003L22 gene mm
MOUSE430A_2:1428745_A_AT RIKEN cDNA 2310003L22 gene mm
MOE430A:1428745_A_AT RIKEN cDNA 2310003L22 gene mm
MG-U74BV2:110812_AT RIKEN cDNA 2310003L22 gene mm
MU19KSUBB:TC34576_AT RIKEN cDNA 2310003L22 gene (2310003L22Rik), mRNA mm
RAE230A:1373899_AT similar to dJ842G6.1.1 (novel protein) (predicted) rn
RAT230_2:1373899_AT similar to dJ842G6.1.1 (novel protein) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAB70169 Hypothetical protein K09E4.3 [Caenorhabditis elegans] ref|NP_496949.1| K09E4.3 [Caenorhabditis elegans] 0.0
blast CAE73406 Hypothetical protein CBG20848 [Caenorhabditis briggsae] 1.0E-172
blast CAE73406 Hypothetical protein CBG20848 [Caenorhabditis briggsae] 1.0E-162
blast NP_001016398 hypothetical protein LOC549152 [Xenopus tropicalis] 1.0E-79
blast NP_077025 hypothetical protein LOC79133 isoform 1 [Homo sapiens] emb|CAI15136.1| chromosome 20 open reading frame 7 [Homo sapiens] emb|CAI22985.1| chromosome 20 open reading frame 7 [Homo sapiens] 4.0E-79
Sequence
Target Sequence 
>C. ELEGANS:178023_S_AT
gatcaaccacctacctcaatggatgcgaaaatgtcatgatatcgtgaagcctgattgccc
attcatcggctcaatgctcgccgaggacacgctctacgagctccgatgctctctgcagct
cgccgagctggaacgcatcggcggagtttcttcgcatatctcgccgtttgttaagacgca
ggatattggaggacttttgtcgtcggcgggttttgatatgattactcttgattcggatga
gattcaagttggatatccgaatatgtttgcgttgatgtatgatcttcagttgatgggaga
atcacattgtacacatcggagaaatccgacgattcggagggatgttctcgtggcggctga
ggctatttatcagtcaatgtactcatccgacggaaaatacccggccacattcaaaatcgt
gtcatttatcggctggaagcctggcccgaatatgccaaaagcagcgaaacgtggatcgca
gacagtttctct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCAACCACCTACCTCAATGGATG 499 421 381 Antisense
GATATCGTGAAGCCTGATTGCCCAT 160 427 418 Antisense
CCATTCATCGGCTCAATGCTCGCCG 462 269 439 Antisense
GAACGCATCGGCGGAGTTTCTTCGC 71 347 511 Antisense
TTCGCATATCTCGCCGTTTGTTAAG 350 697 531 Antisense
ATTGGAGGACTTTTGTCGTCGGCGG 659 1 565 Antisense
GATTCGGAGGGATGTTCTCGTGGCG 134 431 711 Antisense
ATACCCGGCCACATTCAAAATCGTG 358 23 777 Antisense
TCGTGTCATTTATCGGCTGGAAGCC 371 603 797 Antisense
GGAAGCCTGGCCCGAATATGCCAAA 310 531 815 Antisense
AACGTGGATCGCAGACAGTTTCTCT 178 143 848 Antisense
Sequence Source Affymetrix Proprietary Database