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[178006_at]

Description F25D1.3 /REP_DB=WormBase Gene ID /WP=CE09628 /TR=Q19778 /GB=CAA98268.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 03      4grp_total > 48      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 188
1 2 3 4 range
A 4.2
(-3)
A 		81.6
(1.3)
P 		112.4
(3)
P 		33.7
(-1.4)
A 		108
B 4.2
(-3)
A 		133.2
(2.4)
P 		147.3
(3)
P 		5.2
(-3)
A 		143
C 6.7
(-3)
A 		121.7
(0.7)
P 		192.1
(3)
P 		61.5
(-1.2)
A 		185
avr 5
(-3) 		112.2
(1.4) 		150.6
(3) 		33.5
(-1.8) 		146

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 185
1 2 3 4 5 6 range
A 115.2
(-0)
P 		113
(-0.1)
P 		110
(-0.2)
P 		203.6
(3)
P 		105
(-0.4)
P 		28.6
(-3)
A 		175
B 120
(0.1)
P 		81.2
(-1.4)
P 		69
(-1.9)
P 		191.8
(3)
P 		102.2
(-0.6)
P 		41
(-3)
A 		151
C 154.8
(3)
P 		120.8
(1.5)
P 		80.5
(-0.3)
M 		89.9
(0.1)
P 		122.6
(1.6)
P 		19
(-3)
A 		136
avr 130
(1.6) 		105
(0.4) 		86.5
(-0.4) 		161.8
(3) 		109.9
(0.6) 		29.5
(-3) 		132
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 1.5
(-3)
A 		17.7
(0.5)
P 		29.4
(3)
P 		4.9
(-2.3)
A 		28
B 1.3
(-3)
A 		32.9
(2.8)
P 		34.1
(3)
P 		1.1
(-3)
A 		33
C 2.2
(-3)
A 		35
(0)
P 		67
(3)
P 		10.4
(-2.2)
A 		65
avr 1.7
(-3) 		28.5
(0.8) 		43.5
(3) 		5.5
(-2.5) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 21.2
(-1.1)
P 		40.2
(1.5)
P 		24
(-0.7)
P 		51.6
(3)
P 		31.7
(0.3)
P 		7
(-3)
A 		45
B 41.7
(1.5)
P 		27
(-1)
P 		16.8
(-2.7)
P 		50.8
(3)
P 		41.5
(1.4)
P 		15.3
(-3)
A 		36
C 57.4
(3)
P 		36.9
(0.6)
P 		20.5
(-1.3)
M 		18.1
(-1.6)
P 		39.7
(0.9)
P 		6.4
(-3)
A 		51
avr 40.1
(3) 		34.7
(1.9) 		20.4
(-0.9) 		40.2
(3) 		37.6
(2.5) 		9.6
(-3) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178006_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12315
Sequence Type Exemplar sequence
Representative Public ID F25D1.3 NCBI
Target Description F25D1.3 /REP_DB=WormBase Gene ID /WP=CE09628 /TR=Q19778 /GB=CAA98268.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073299(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073299 NCBI Caenorhabditis elegans F25D1.3 (F25D1.3) mRNA, complete cds. 11/11 None
SNAP00000015976 ENSEMBL cdna:SNAP chromosome:CEL140:V:10532502:10533036:1 11/11 None
GENEFINDER00000015982 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10532532:10533036:1 10/11 None
F25D1.3 ENSEMBL cdna:known chromosome:CEL140:V:10531335:10533149:1 gene:F25D1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10532501-10533036(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25D1.3
Entrez Gene 179468 Entrez gene
SwissProt Q19778 EMBL-EBI
WormBase CE31917 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98268 Hypothetical protein F25D1.3 [Caenorhabditis elegans] emb|CAD54125.1| Hypothetical protein F25D1.3 [Caenorhabditis elegans] ref|NP_505700.2| F25D1.3 [Caenorhabditis elegans] 1.0E-108
blast CAA98268 Hypothetical protein F25D1.3 [Caenorhabditis elegans] emb|CAD54125.1| Hypothetical protein F25D1.3 [Caenorhabditis elegans] ref|NP_505700.2| F25D1.3 [Caenorhabditis elegans] 2.0E-80
blast CAA98268 Hypothetical protein F25D1.3 [Caenorhabditis elegans] emb|CAD54125.1| Hypothetical protein F25D1.3 [Caenorhabditis elegans] ref|NP_505700.2| F25D1.3 [Caenorhabditis elegans] 7.0E-76
blast CAE64835 Hypothetical protein CBG09631 [Caenorhabditis briggsae] 2.0E-71
blast CAE64835 Hypothetical protein CBG09631 [Caenorhabditis briggsae] 5.0E-71
blast CAE64835 Hypothetical protein CBG09631 [Caenorhabditis briggsae] 3.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR010345 EMBL-EBI Interleukin-17 5.4E-83
Pfam IPR010345 EMBL-EBI Interleukin-17 2.6E-61
Pfam IPR010345 EMBL-EBI Interleukin-17 1.7E-58
Sequence
Target Sequence 
>C. ELEGANS:178006_AT
aagaggatcttgctacatggatgcaaatttcaatgagtggtcaatttgatgatacagcat
tagaggaatggagtacaaatggtaaagaacctgagatctgtgagaaatctccaaaagctg
atggagttactacgattatggagagagctctatgtccatgggatagcagagtcaactacc
aagagagccgagaacctaaattgattgctgaatcagtttgtctatgccgtaagagccgtg
gttctacaggagctttctgtatgccaattgttcgaaaagttccaattctccgacgtgtct
cttgtgatcgttcaacaggtttatggaattatgtaagatcaactgagctaataactgttg
gatgtcattctgtattgccaagaactcaaagagcagcacgtcttgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAGGATCTTGCTACATGGATGCA 647 145 23 Antisense
GAGAGAGCTCTATGTCCATGGGATA 33 395 163 Antisense
GCAGAGTCAACTACCAAGAGAGCCG 256 313 188 Antisense
GAGAGCCGAGAACCTAAATTGATTG 241 395 205 Antisense
GCTGAATCAGTTTGTCTATGCCGTA 124 297 229 Antisense
GTCTATGCCGTAAGAGCCGTGGTTC 551 467 242 Antisense
GAGCCGTGGTTCTACAGGAGCTTTC 215 385 255 Antisense
GGAGCTTTCTGTATGCCAATTGTTC 539 519 271 Antisense
TCCGACGTGTCTCTTGTGATCGTTC 419 595 311 Antisense
CTGTTGGATGTCATTCTGTATTGCC 116 239 377 Antisense
AACTCAAAGAGCAGCACGTCTTGCC 690 139 405 Antisense
Sequence Source Affymetrix Proprietary Database