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[178002_at]

Description F25D1.2 /REP_DB=WormBase Gene ID /WP=CE05723 /TR=Q19776 /GB=CAA98266.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 01      6grp_high1_10_10 > 01      4grp_high_8_8 > 19      6grp_highest_nonorm > 90      6grp_total > 08      4grp_total > 21      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 94
(-0.4)
P 		91.1
(-0.7)
P 		73.9
(-3)
M 		119.6
(3)
P 		46
B 122.9
(3)
P 		89.6
(-0.5)
P 		66.4
(-3)
P 		122.2
(2.9)
P 		57
C 98.8
(1.4)
P 		72.5
(-0.8)
P 		45.4
(-3)
P 		118.3
(3)
P 		73
avr 105.2
(1.5) 		84.4
(-0.7) 		61.9
(-3) 		120
(3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 132
(3)
P 		42.3
(-3)
P 		83.6
(-0.2)
P 		66.8
(-1.4)
P 		64.8
(-1.5)
P 		47.9
(-2.6)
P 		90
B 95.5
(3)
P 		77
(0.7)
P 		72.1
(0.1)
P 		65
(-0.8)
P 		49
(-2.8)
P 		47.5
(-3)
P 		48
C 85.4
(0.9)
P 		76.5
(-0.1)
P 		74.3
(-0.4)
P 		102.4
(3)
P 		82.1
(0.5)
P 		52.9
(-3)
P 		50
avr 104.3
(3) 		65.3
(-1.3) 		76.7
(-0) 		78.1
(0.1) 		65.3
(-1.3) 		49.4
(-3) 		55
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 34.2
(3)
P 		19.8
(-2.2)
P 		19.3
(-2.4)
M 		17.5
(-3)
P 		17
B 39.2
(3)
P 		22.1
(-1.3)
P 		15.4
(-3)
P 		26
(-0.3)
P 		24
C 32.7
(3)
P 		20.8
(-1.2)
P 		15.8
(-3)
P 		19.9
(-1.5)
P 		17
avr 35.4
(3) 		20.9
(-1.7) 		16.8
(-3) 		21.1
(-1.6) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 24.3
(3)
P 		15.1
(-1.4)
P 		18.2
(0.1)
P 		16.9
(-0.5)
P 		19.5
(0.7)
P 		11.7
(-3)
P 		13
B 33.2
(3)
P 		25.6
(0.2)
P 		17.5
(-2.9)
P 		17.2
(-3)
P 		19.9
(-2)
P 		17.7
(-2.8)
P 		16
C 31.7
(3)
P 		23.4
(-0.6)
P 		18.9
(-2.5)
P 		20.6
(-1.8)
P 		26.6
(0.8)
P 		17.7
(-3)
P 		14
avr 29.7
(3) 		21.4
(-0.6) 		18.2
(-1.9) 		18.2
(-1.9) 		22
(-0.3) 		15.7
(-3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178002_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12436
Sequence Type Exemplar sequence
Representative Public ID F25D1.2 NCBI
Target Description F25D1.2 /REP_DB=WormBase Gene ID /WP=CE05723 /TR=Q19776 /GB=CAA98266.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073300(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073300 NCBI Caenorhabditis elegans F25D1.2 (F25D1.2) mRNA, complete cds. 11/11 None
SNAP00000015977 ENSEMBL cdna:SNAP chromosome:CEL140:V:10533679:10535406:-1 11/11 None
F25D1.2 ENSEMBL cdna:known chromosome:CEL140:V:10533679:10535406:-1 gene:F25D1.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000015984 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10533678-10535406(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25D1.2
Entrez Gene 184920 Entrez gene
SwissProt Q19776 EMBL-EBI
WormBase CE05723 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98266 Hypothetical protein F25D1.2 [Caenorhabditis elegans] ref|NP_505701.1| F25D1.2 [Caenorhabditis elegans] 0.0
blast CAE64836 Hypothetical protein CBG09632 [Caenorhabditis briggsae] 1.0E-152
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505701.1 9 29-51,64-86,90-112,119-141,145-164,194-216,231-253,260-282,292-314
F25D1.2 9 29-51,64-86,90-112,119-141,145-164,194-216,231-253,260-282,292-314
SNAP00000015977 9 29-51,64-86,90-112,119-141,145-164,194-216,231-253,260-282,292-314
Sequence
Target Sequence 
>C. ELEGANS:178002_AT
tttctgcaggtcttactgcttctgcaggaattctatacttgattcttcgtcccgatgaat
atccaacagaagaatgtactgaaactgtatcaatcgacggaaatactccagaaccagtta
aaagttctcatcctgctctctttgttttcctattgatggtcattaatgtttcgattggag
ttccattaatgcagacatacagtgctctcatattcacttattatggaatgacaacacatt
ctgcattacttttttcagtgttctacccaattcttcaattgattccagttatggtgtcaa
caagagttaatatttccagaagaattcttattctcggaggatactttatttccattaaaa
ttcaattctttcttatcataacttcatcgtacccatatcttccaccaaaccatcaaatga
ttgcaatgactgtacttctactaattctatcaatttctttcacttttccatgcaacactg
ctctttgtatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTCTGCAGGTCTTACTGCTTCTGC 230 671 458 Antisense
ATTCTATACTTGATTCTTCGTCCCG 595 9 487 Antisense
TCTTCGTCCCGATGAATATCCAACA 605 617 501 Antisense
AGTTAAAAGTTCTCATCCTGCTCTC 643 63 573 Antisense
TGCTCTCTTTGTTTTCCTATTGATG 159 581 591 Antisense
GCAGACATACAGTGCTCTCATATTC 280 313 648 Antisense
CAACACATTCTGCATTACTTTTTTC 179 189 689 Antisense
TTTTTCAGTGTTCTACCCAATTCTT 489 675 708 Antisense
ATCATAACTTCATCGTACCCATATC 631 29 832 Antisense
ATTTCTTTCACTTTTCCATGCAACA 107 17 910 Antisense
TCCATGCAACACTGCTCTTTGTATT 292 591 924 Antisense
Sequence Source Affymetrix Proprietary Database