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[177989_at]

Description F46A8.6 /REP_DB=WormBase Gene ID /WP=CE10568 /TR=O01332 /GB=CAB04392.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 79      4grp_total > 50      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 125
1 2 3 4 range
A 76.9
(1)
A 		102.9
(3)
A 		70.8
(0.6)
A 		24.2
(-3)
A 		79
B 38.1
(-1.5)
A 		132.3
(3)
A 		100.8
(1.5)
A 		6.9
(-3)
A 		125
C 65.6
(-3)
A 		102.4
(0.6)
A 		89.8
(-0.6)
A 		127
(3)
A 		61
avr 60.2
(-2.2) 		112.5
(3) 		87.1
(0.5) 		52.7
(-3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 71.9
(0.8)
A 		92.3
(3)
A 		39
(-2.8)
A 		37.6
(-3)
A 		91.6
(2.9)
P 		70
(0.6)
P 		55
B 30.1
(-3)
A 		73.7
(2.5)
A 		61.9
(1)
A 		57.9
(0.5)
A 		61.6
(1)
M 		77.6
(3)
P 		48
C 64
(0.1)
P 		39.4
(-3)
A 		86.4
(3)
A 		62.6
(-0)
A 		60.2
(-0.3)
M 		75.4
(1.6)
A 		47
avr 55.3
(-2.3) 		68.5
(1.4) 		62.4
(-0.3) 		52.7
(-3) 		71.1
(2.1) 		74.3
(3) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 28
(3)
A 		22.3
(1.6)
A 		18.5
(0.7)
A 		3.5
(-3)
A 		25
B 12.2
(-0.9)
A 		32.7
(3)
A 		23.4
(1.2)
A 		1.5
(-3)
A 		31
C 21.7
(-2.8)
A 		29.4
(1.8)
A 		31.3
(3)
A 		21.4
(-3)
A 		10
avr 20.6
(0.7) 		28.1
(3) 		24.4
(1.8) 		8.8
(-3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 13.2
(-1.8)
A 		32.8
(3)
A 		8.5
(-3)
A 		9.5
(-2.8)
A 		27.6
(1.7)
P 		17
(-0.9)
P 		24
B 10.5
(-3)
A 		24.5
(1.5)
A 		15
(-1.5)
A 		15.3
(-1.4)
A 		25
(1.7)
M 		29
(3)
P 		19
C 23.7
(2.3)
P 		12
(-3)
A 		22
(1.5)
A 		12.6
(-2.7)
A 		19.5
(0.4)
M 		25.3
(3)
A 		13
avr 15.8
(-1.3) 		23.1
(2.5) 		15.2
(-1.6) 		12.5
(-3) 		24
(3) 		23.8
(2.9) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177989_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.578
Sequence Type Exemplar sequence
Representative Public ID F46A8.6 NCBI
Target Description F46A8.6 /REP_DB=WormBase Gene ID /WP=CE10568 /TR=O01332 /GB=CAB04392.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060485(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060485 NCBI Caenorhabditis elegans F46A8.6 (F46A8.6) mRNA, complete cds. 11/11 None
GENEFINDER00000031227 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11247840:11253172:-1 9/11 None
SNAP00000031222 ENSEMBL cdna:SNAP chromosome:CEL140:I:11251998:11253172:-1 9/11 None
F46A8.6 ENSEMBL cdna:known chromosome:CEL140:I:11251960:11253127:-1 gene:F46A8.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000034596 1/11 Cross Hyb Matching Probes None
GENEFINDER00000034599 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4142526-4142973(-) 60.95 93.53
chrI:11252608-11253058(-) 67.41 100.0
Public Domain and Genome References
Gene Title
Ensembl F46A8.6
Entrez Gene 185822 Entrez gene
SwissProt O01332 EMBL-EBI
WormBase CE34187 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04389 Hypothetical protein F46A8.3 [Caenorhabditis elegans] ref|NP_492885.1| F46A8.3 [Caenorhabditis elegans] 4.0E-87
blast CAB04392 Hypothetical protein F46A8.6 [Caenorhabditis elegans] ref|NP_492886.2| F46A8.6 [Caenorhabditis elegans] 1.0E-79
blast AAF99967 Hypothetical protein F49F1.11 [Caenorhabditis elegans] ref|NP_500492.1| F49F1.11 [Caenorhabditis elegans] 6.0E-79
blast CAB04391 Hypothetical protein F46A8.5 [Caenorhabditis elegans] ref|NP_492883.1| F46A8.5 [Caenorhabditis elegans] 9.0E-76
blast CAB04392 Hypothetical protein F46A8.6 [Caenorhabditis elegans] ref|NP_492886.2| F46A8.6 [Caenorhabditis elegans] 2.0E-45
blast CAB55058 Hypothetical protein Y50E8A.10 [Caenorhabditis elegans] ref|NP_506647.1| Y50E8A.10 [Caenorhabditis elegans] 6.0E-18
blast AAF99960 Hypothetical protein F49F1.12 [Caenorhabditis elegans] ref|NP_500493.1| F49F1.12 [Caenorhabditis elegans] 5.0E-10
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000031227 1 7-26
SNAP00000031222 1 7-26
Sequence
Target Sequence 
>C. ELEGANS:177989_AT
ctacaattactacaaactactttcagagtggaatcctggtcatgtgcttttgcaagaaga
agcctcctaagagccagtgtcctgtgcgaccaatgacgatgtcgatgagagaggcgcagg
tatctgatgtggggccggacagcacgcggacagcgatggacaatcagaaaaagagcaaga
agaagaagagcaaggagccagccaagaagaagaagaaatcggcggattcttcgcagaagg
tctcgaagaagaagaagaagtcgagcaaatcgaagaagtcggcggagaagaagaagccgg
aggtggtggcaccaatggacccggatgtcaagtccacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTACAATTACTACAAACTACTTTCA 439 223 47 Antisense
ACTACTTTCAGAGTGGAATCCTGGT 13 101 62 Antisense
GAATCCTGGTCATGTGCTTTTGCAA 175 331 77 Antisense
AGAAGCCTCCTAAGAGCCAGTGTCC 55 75 104 Antisense
CCTAAGAGCCAGTGTCCTGTGCGAC 35 265 112 Antisense
GTCCTGTGCGACCAATGACGATGTC 239 477 125 Antisense
GATGAGAGAGGCGCAGGTATCTGAT 328 411 150 Antisense
GGTATCTGATGTGGGGCCGGACAGC 549 503 165 Antisense
GGACAGCACGCGGACAGCGATGGAC 674 525 183 Antisense
GAGGTGGTGGCACCAATGGACCCGG 224 403 346 Antisense
AATGGACCCGGATGTCAAGTCCACG 698 161 360 Antisense
Sequence Source Affymetrix Proprietary Database