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[177977_s_at]

Description C34D1.4 /REP_DB=WormBase Gene ID /WP=CE23568 /TR=Q18433 /GB=CAB01488.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 79      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 83      6grp_total > 89      4grp_total > 03      6grp_highest_sub > Da      6grp_high1_sub > Cu      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1544
1 2 3 4 range
A 513.6
(-1.9)
P 		238.2
(-3)
P 		355.5
(-2.5)
P 		1782.4
(3)
P 		1544
B 439.1
(-2.1)
P 		292
(-2.8)
P 		245.6
(-3)
P 		1505.9
(3)
P 		1260
C 502.4
(-1.8)
P 		393.3
(-2.3)
P 		263.2
(-3)
P 		1456.1
(3)
P 		1193
avr 485
(-2.1) 		307.8
(-2.9) 		288.1
(-3) 		1581.5
(3) 		1293

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 214
1 2 3 4 5 6 range
A 316.7
(-3)
P 		358.8
(-1.8)
P 		375.5
(-1.4)
P 		336.8
(-2.4)
P 		474.8
(1.4)
P 		530.9
(3)
P 		214
B 480.5
(1.7)
P 		358.5
(-2.2)
P 		332.8
(-3)
P 		409.6
(-0.6)
P 		521.8
(3)
P 		509.2
(2.6)
P 		189
C 386.6
(-1.4)
P 		493.3
(2.1)
P 		339.4
(-3)
P 		407.2
(-0.7)
P 		418.4
(-0.4)
P 		519.2
(3)
P 		180
avr 394.6
(-1.4) 		403.5
(-1.1) 		349.2
(-3) 		384.5
(-1.8) 		471.7
(1.3) 		519.8
(3) 		171
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 269
1 2 3 4 range
A 187
(0.9)
P 		51.7
(-3)
P 		92.9
(-1.8)
P 		260.9
(3)
P 		209
B 140
(-1.1)
P 		72.1
(-2.7)
P 		56.9
(-3)
P 		320.9
(3)
P 		264
C 166.3
(-0.1)
P 		113.1
(-2.2)
P 		91.7
(-3)
P 		245.5
(3)
P 		154
avr 164.4
(-0.4) 		79
(-3) 		80.5
(-3) 		275.8
(3) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 154
1 2 3 4 5 6 range
A 58.4
(-3)
P 		127.6
(1.9)
P 		81.8
(-1.3)
P 		85.4
(-1.1)
P 		143.3
(3)
P 		129.3
(2)
P 		85
B 167.2
(1)
P 		119.3
(-1.2)
P 		80.8
(-3)
P 		108.5
(-1.7)
P 		212
(3)
P 		189.9
(2)
P 		131
C 143.3
(1)
P 		150.5
(1.5)
P 		86.4
(-2.7)
P 		81.9
(-3)
P 		135.4
(0.5)
P 		173.8
(3)
P 		92
avr 123
(-0) 		132.5
(0.7) 		83
(-3) 		91.9
(-2.3) 		163.6
(2.9) 		164.3
(3) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177977_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12974
Sequence Type Exemplar sequence
Representative Public ID C34D1.4 NCBI
Target Description C34D1.4 /REP_DB=WormBase Gene ID /WP=CE23568 /TR=Q18433 /GB=CAB01488.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073890(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073890 NCBI Caenorhabditis elegans C34D1.4 (C34D1.4) mRNA, complete cds. 11/11 A 
GENEFINDER00000025084 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13258673:13261883:-1 10/11 None
C34D1.4 ENSEMBL cdna:known chromosome:CEL140:V:13259694:13261611:-1 gene:C34D1.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000025076 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13259813-13261611(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C34D1.4
Entrez Gene 179807 Entrez gene
SwissProt Q18433 EMBL-EBI
WormBase CE23568 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01488 Hypothetical protein C34D1.4 [Caenorhabditis elegans] ref|NP_506291.1| C34D1.4 [Caenorhabditis elegans] 1.0E-110
blast CAE64643 Hypothetical protein CBG09407 [Caenorhabditis briggsae] 3.0E-93
blast CAE64643 Hypothetical protein CBG09407 [Caenorhabditis briggsae] 1.0E-75
blast CAB01488 Hypothetical protein C34D1.4 [Caenorhabditis elegans] ref|NP_506291.1| C34D1.4 [Caenorhabditis elegans] 3.0E-73
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506291.1 4 21-40,44-61,68-90,130-152
C34D1.4 4 21-40,44-61,68-90,130-152
GENEFINDER00000025084 4 51-70,75-92,99-121,161-183
Sequence
Target Sequence 
>C. ELEGANS:177977_S_AT
ggaaaagcattaccgtcacctgattttttccttatcacttcatacgctcatctaccttct
tctacatatgtgtttttcccctcgtgatcttgctcaagcttacctcgctgatgttatcgt
tctccttttggctgcttttggtactgcgcgtaatctacccattctcatctttcccagtgt
tatagtcaagttcatatgtttctcactttgtgctggtgtagccatgttgtctctaagcga
ga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAAAAGCATTACCGTCACCTGATT 144 529 54 Antisense
GTCACCTGATTTTTTCCTTATCACT 567 465 68 Antisense
ATTTTTTCCTTATCACTTCATACGC 298 5 76 Antisense
CTCATCTACCTTCTTCTACATATGT 610 229 100 Antisense
CTCAAGCTTACCTCGCTGATGTTAT 677 227 146 Antisense
ATTCTCATCTTTCCCAGTGTTATAG 701 9 214 Antisense
TTCCCAGTGTTATAGTCAAGTTCAT 331 697 224 Antisense
GTTCATATGTTTCTCACTTTGTGCT 239 445 243 Antisense
GTTTCTCACTTTGTGCTGGTGTAGC 275 451 251 Antisense
GTGCTGGTGTAGCCATGTTGTCTCT 37 479 263 Antisense
GTAGCCATGTTGTCTCTAAGCGAGA 70 457 271 Antisense
Sequence Source Affymetrix Proprietary Database