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[177976_at]

Description C34D1.4 /REP_DB=WormBase Gene ID /WP=CE23568 /TR=Q18433 /GB=CAB01488.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 89      4grp_highest_8_8 > 23      4grp_high_8_8 > 05      6grp_highest_nonorm > 91      6grp_total > 98      4grp_total > 03      6grp_highest_sub > In      6grp_high1_sub > Cu      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1036
1 2 3 4 range
A 207.9
(-2.2)
P 		212.2
(-2.1)
P 		73
(-3)
P 		1043
(3)
P 		970
B 280.3
(-2.1)
P 		139.4
(-3)
P 		137.2
(-3)
P 		1108.9
(3)
P 		972
C 285.6
(-1.9)
P 		141.3
(-3)
P 		154.1
(-2.9)
P 		945.1
(3)
P 		804
avr 257.9
(-2.1) 		164.3
(-2.7) 		121.4
(-3) 		1032.3
(3) 		911

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 229
1 2 3 4 5 6 range
A 186.7
(-3)
P 		197.4
(-2.4)
P 		230.7
(-0.7)
P 		228.3
(-0.8)
P 		301.9
(3)
P 		277.5
(1.7)
P 		115
B 233
(-1.6)
P 		196.1
(-3)
P 		200.7
(-2.8)
P 		219.5
(-2.1)
P 		350.6
(3)
P 		296.8
(0.9)
P 		155
C 255.3
(-1.5)
P 		200
(-3)
P 		206.3
(-2.8)
P 		227.2
(-2.2)
P 		300.8
(-0.2)
P 		415.4
(3)
P 		215
avr 225
(-1.8) 		197.8
(-3) 		212.6
(-2.3) 		225
(-1.8) 		317.8
(2.5) 		329.9
(3) 		132
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 217
1 2 3 4 range
A 75.7
(-0.5)
P 		46.1
(-1.8)
P 		19.1
(-3)
P 		152.7
(3)
P 		134
B 89.4
(-1.3)
P 		34.4
(-2.9)
P 		31.8
(-3)
P 		236.3
(3)
P 		205
C 94.5
(-0.3)
P 		40.6
(-3)
P 		53.7
(-2.3)
P 		159.4
(3)
P 		119
avr 86.5
(-0.9) 		40.4
(-2.8) 		34.9
(-3) 		182.8
(3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 34.4
(-3)
P 		70.2
(0.8)
P 		50.2
(-1.3)
P 		57.9
(-0.5)
P 		91.1
(3)
P 		67.6
(0.5)
P 		57
B 81.1
(-0.9)
P 		65.3
(-1.9)
P 		48.7
(-3)
P 		58.1
(-2.4)
P 		142.5
(3)
P 		110.7
(1)
P 		94
C 94.6
(0.1)
P 		61
(-2)
P 		52.5
(-2.6)
P 		45.7
(-3)
P 		97.3
(0.3)
P 		139.1
(3)
P 		93
avr 70
(-1) 		65.5
(-1.5) 		50.5
(-3) 		53.9
(-2.7) 		110.3
(3) 		105.8
(2.5) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177976_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12974
Sequence Type Exemplar sequence
Representative Public ID C34D1.4 NCBI
Target Description C34D1.4 /REP_DB=WormBase Gene ID /WP=CE23568 /TR=Q18433 /GB=CAB01488.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073890(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073890 NCBI Caenorhabditis elegans C34D1.4 (C34D1.4) mRNA, complete cds. 11/11 A 
SNAP00000025076 ENSEMBL cdna:SNAP chromosome:CEL140:V:13259814:13260930:-1 10/11 None
C34D1.4 ENSEMBL cdna:known chromosome:CEL140:V:13259694:13261611:-1 gene:C34D1.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000025084 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13259813-13261611(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C34D1.4
Entrez Gene 179807 Entrez gene
SwissProt Q18433 EMBL-EBI
WormBase CE23568 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01488 Hypothetical protein C34D1.4 [Caenorhabditis elegans] ref|NP_506291.1| C34D1.4 [Caenorhabditis elegans] 1.0E-110
blast CAB01488 Hypothetical protein C34D1.4 [Caenorhabditis elegans] ref|NP_506291.1| C34D1.4 [Caenorhabditis elegans] 4.0E-98
blast CAE64643 Hypothetical protein CBG09407 [Caenorhabditis briggsae] 3.0E-93
blast CAE64643 Hypothetical protein CBG09407 [Caenorhabditis briggsae] 8.0E-89
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506291.1 4 21-40,44-61,68-90,130-152
C34D1.4 4 21-40,44-61,68-90,130-152
SNAP00000025076 3 10-27,32-54,94-116
Sequence
Target Sequence 
>C. ELEGANS:177976_AT
cttctacatatgtgtttttcccctcgtgatcttgctcaagcttacctcgctgatgttatc
gttctccttttggctgcttttggtactgcgcgtaatctacccattctcatctttcccagt
gttatagtcaagttcatatgtttctcactttgtgctggtgtagccatgttgtctctaagc
gagacgagtatcaatatgaaaacaaaagaagcacggatggagttcagacgacaaagaaat
tatcctgaagttgtaaatattgcatttgaagatcatccgacagtttgcatttggattcac
gcaatggctgtaattgttgcattagatttgaatatcggattgaaagctttttatagctct
ttcgattgcatgaagaaaccagttgtcgaagaggagaaacctccatcatatacagtttgc
atgacaaattcctcacaaactaccttaccaccaacttacgaagaagcactggaagctcga
aagaaaatgcaagctcaagcttttccaacaagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCTACATATGTGTTTTTCCCCTC 432 215 112 Antisense
AGCACGGATGGAGTTCAGACGACAA 391 77 321 Antisense
GAAGATCATCCGACAGTTTGCATTT 684 337 379 Antisense
ATTTGGATTCACGCAATGGCTGTAA 271 17 400 Antisense
TTTTATAGCTCTTTCGATTGCATGA 698 673 460 Antisense
GATTGCATGAAGAAACCAGTTGTCG 157 435 475 Antisense
GGAGAAACCTCCATCATATACAGTT 22 519 504 Antisense
TGACAAATTCCTCACAAACTACCTT 623 565 533 Antisense
CTTACCACCAACTTACGAAGAAGCA 496 219 555 Antisense
GAAGAAGCACTGGAAGCTCGAAAGA 631 339 571 Antisense
CAAGCTCAAGCTTTTCCAACAAGGA 312 185 601 Antisense
Sequence Source Affymetrix Proprietary Database