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[177974_s_at]

Description C06A1.4 /REP_DB=WormBase Gene ID /WP=CE02980 /TR=Q17685 /GB=CAA90054.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 60      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 113
1 2 3 4 range
A 90.5
(3)
A 		78.3
(-3)
A 		90.6
(3)
A 		88.3
(1.9)
A 		12
B 88.5
(1.2)
A 		116.3
(3)
A 		112
(2.7)
A 		24.5
(-3)
A 		92
C 117.5
(1.7)
A 		113.3
(1.4)
A 		137.7
(3)
A 		45
(-3)
A 		93
avr 98.8
(1.6) 		102.6
(1.9) 		113.4
(3) 		52.6
(-3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 678
1 2 3 4 5 6 range
A 28.1
(-3)
A 		90.5
(-2.4)
A 		69.9
(-2.6)
A 		197.4
(-1.5)
A 		475
(1)
A 		697.8
(3)
P 		670
B 59.5
(-2.6)
A 		19.6
(-3)
A 		86.4
(-2.3)
A 		195
(-1.3)
A 		432.3
(1.1)
A 		624
(3)
P 		604
C 80.3
(-2.8)
A 		61.1
(-3)
A 		148.9
(-2)
A 		113.2
(-2.4)
A 		534.3
(2.6)
P 		569.8
(3)
P 		509
avr 56
(-3) 		57.1
(-3) 		101.7
(-2.5) 		168.5
(-1.8) 		480.5
(1.4) 		630.5
(3) 		575
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 33
(3)
A 		17
(-1.8)
A 		23.7
(0.2)
A 		12.9
(-3)
A 		20
B 28.2
(2.9)
A 		28.7
(3)
A 		26
(2.3)
A 		5.2
(-3)
A 		24
C 38.9
(1.6)
A 		32.6
(0.7)
A 		48
(3)
A 		7.6
(-3)
A 		40
avr 33.4
(3) 		26.1
(1.2) 		32.6
(2.8) 		8.6
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 228
1 2 3 4 5 6 range
A 5.2
(-3)
A 		32.2
(-2)
A 		15.2
(-2.6)
A 		50.1
(-1.4)
A 		143.3
(2)
A 		169.9
(3)
P 		165
B 20.7
(-2.6)
A 		6.5
(-3)
A 		21
(-2.6)
A 		51.6
(-1.8)
A 		175.6
(1.5)
A 		232.7
(3)
P 		226
C 29.8
(-2.6)
A 		18.6
(-3)
A 		37.9
(-2.3)
A 		22.8
(-2.9)
A 		172.9
(2.4)
P 		190.7
(3)
P 		172
avr 18.6
(-3) 		19.1
(-3) 		24.7
(-2.8) 		41.5
(-2.2) 		163.9
(1.9) 		197.8
(3) 		179
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177974_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3476
Sequence Type Exemplar sequence
Representative Public ID C06A1.4 NCBI
Target Description C06A1.4 /REP_DB=WormBase Gene ID /WP=CE02980 /TR=Q17685 /GB=CAA90054.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064350(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064350 NCBI Caenorhabditis elegans F58G1.1 (F58G1.1) mRNA, complete cds. 11/11 A 
SNAP00000003685 ENSEMBL cdna:SNAP chromosome:CEL140:II:12919474:12922829:1 11/11 None
GENEFINDER00000003700 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12919474:12922829:1 11/11 None
SNAP00000011345 ENSEMBL cdna:SNAP chromosome:CEL140:II:10574753:10578668:-1 11/11 None
GENEFINDER00000011347 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10574753:10578104:-1 11/11 None
C06A1.4 ENSEMBL cdna:pseudogene chromosome:CEL140:II:10574753:10578104:-1 gene:C06A1.4 11/11 A 
F58G1.1 ENSEMBL cdna:known chromosome:CEL140:II:12919474:12923072:1 gene:F58G1.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12919470-12922826(+) 92.16 92.16
chrII:10574749-10578101(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C06A1.4
F58G1.1
Entrez Gene 174932 Entrez gene
SwissProt O62275 EMBL-EBI
WormBase CE17921 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04524 Hypothetical protein F58G1.1 [Caenorhabditis elegans] ref|NP_496751.1| F58G1.1 [Caenorhabditis elegans] 0.0
blast T18974 hypothetical protein C06A1.4 - Caenorhabditis elegans 0.0
blast AAF60414 Paz/piwi domain-containing protein 2 [Caenorhabditis elegans] ref|NP_491535.1| PAZ/PIWI domain-containing family member (ppw-2) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 8.5E-54
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 8.5E-54
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 3.4E-50
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 3.4E-50
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:177974_S_AT
gaaatcatggatcaatcatggcctatgagattcctcttgttcgtgttgccatgagaggtt
tctcacctgaaattcaactggtctacatcgtggtgtcaaaggatcacagcttcagattct
tcaagccggatgtgacaagtctagctgcccgtccacaagcgacttccagcaccgcaagca
gacattctgcaatgccagccgctccaaaagcatgggatctgaatattgctcccggtatta
tggtcgattccatcgtgacaaatccggcctgcaagcaatttttcctgaattcccacatca
ctctgcaaggaactgcgaaaactccattgtacacagttctcgccgatgacgccaaagtca
gcatgacggcattggaggatatcacgtacaagctgtgccatcttcaccagattgttggac
ttccgacgtcgcttccaacttcattgtatgtcgccaacgagtacgccaagagaggacgca
atctgtggaacgaggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATCATGGATCAATCATGGCCTA 391 363 2216 Antisense
GGTGTCAAAGGATCACAGCTTCAGA 566 499 2307 Antisense
GGTCGATTCCATCGTGACAAATCCG 275 503 2457 Antisense
AATTCCCACATCACTCTGCAAGGAA 695 177 2503 Antisense
TTCTCGCCGATGACGCCAAAGTCAG 594 693 2552 Antisense
GCCGATGACGCCAAAGTCAGCATGA 670 283 2557 Antisense
GAGGATATCACGTACAAGCTGTGCC 228 403 2590 Antisense
CACGTACAAGCTGTGCCATCTTCAC 535 197 2598 Antisense
TCGCCAACGAGTACGCCAAGAGAGG 409 599 2666 Antisense
GAGTACGCCAAGAGAGGACGCAATC 169 395 2674 Antisense
GAGGACGCAATCTGTGGAACGAGGC 117 405 2687 Antisense
Sequence Source Affymetrix Proprietary Database