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[177972_at]

Description ZK520.3 /REP_DB=WormBase Gene ID /WP=CE16728 /TR=O46020 /GB=CAB07301.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 26      4grp_total > 37      6grp_total_sub > Cup      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 99.1
(3)
A 		47.5
(-0.7)
A 		15.4
(-3)
A 		50.7
(-0.5)
A 		84
B 128.7
(3)
P 		71.4
(0)
A 		12.3
(-3)
A 		48.9
(-1.1)
A 		116
C 98.6
(1.9)
A 		104.8
(3)
A 		69.8
(-3)
A 		87.9
(0.1)
A 		35
avr 108.8
(3) 		74.6
(0.3) 		32.5
(-3) 		62.5
(-0.6) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 79
(2.7)
A 		81.9
(3)
A 		24.9
(-3)
A 		38.3
(-1.6)
A 		37.9
(-1.6)
A 		61.1
(0.8)
A 		57
B 97.9
(3)
A 		66.6
(0.8)
A 		14.2
(-2.8)
A 		59.4
(0.3)
A 		18.5
(-2.5)
A 		10.8
(-3)
A 		87
C 65.3
(1.5)
A 		85
(3)
A 		61.5
(1.2)
A 		6.7
(-3)
A 		17.4
(-2.2)
A 		21.6
(-1.9)
A 		78
avr 80.7
(3) 		77.8
(2.7) 		33.5
(-2) 		34.8
(-1.9) 		24.6
(-3) 		31.2
(-2.3) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 36.1
(3)
A 		10.3
(-1.8)
A 		4
(-3)
A 		7.4
(-2.4)
A 		32
B 41.1
(3)
P 		17.6
(-0.7)
A 		2.8
(-3)
A 		10.4
(-1.8)
A 		38
C 32.6
(3)
A 		30.1
(2.2)
A 		24.3
(0.2)
A 		14.8
(-3)
A 		18
avr 36.6
(3) 		19.3
(-1) 		10.4
(-3) 		10.9
(-2.9) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 14.6
(-0.7)
A 		29.1
(3)
A 		5.4
(-3)
A 		9.7
(-1.9)
A 		11.4
(-1.5)
A 		14.9
(-0.6)
A 		24
B 34.1
(3)
A 		22.2
(0.7)
A 		3.5
(-3)
A 		15.7
(-0.6)
A 		7.5
(-2.2)
A 		4
(-2.9)
A 		31
C 24.2
(2.6)
A 		25.9
(3)
A 		15.7
(0.5)
A 		1.3
(-3)
A 		5.6
(-2)
A 		7.2
(-1.6)
A 		25
avr 24.3
(2.5) 		25.7
(3) 		8.2
(-3) 		8.9
(-2.8) 		8.2
(-3) 		8.7
(-2.8) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177972_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7320
Sequence Type Exemplar sequence
Representative Public ID ZK520.3 NCBI
Target Description ZK520.3 /REP_DB=WormBase Gene ID /WP=CE16728 /TR=O46020 /GB=CAB07301.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067423(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067423 NCBI Caenorhabditis elegans ZK520.3 (ZK520.3) mRNA, complete cds. 11/11 None
SNAP00000038071 ENSEMBL cdna:SNAP chromosome:CEL140:III:13675769:13684457:-1 11/11 None
GENEFINDER00000038077 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13681940:13685961:-1 11/11 None
ZK520.3 ENSEMBL cdna:known chromosome:CEL140:III:13681936:13685961:-1 gene:ZK520.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13681931-13685957(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK520.3
Entrez Gene 191342 Entrez gene
SwissProt O46020 EMBL-EBI
WormBase CE16728 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07301 Hypothetical protein ZK520.3 [Caenorhabditis elegans] ref|NP_499824.1| ZK520.3 [Caenorhabditis elegans] 0.0
blast CAE71377 Hypothetical protein CBG18281 [Caenorhabditis briggsae] 0.0
blast CAB07299 Hypothetical protein ZK520.1 [Caenorhabditis elegans] emb|CAD90189.1| Hypothetical protein ZK520.1 [Caenorhabditis elegans] ref|NP_499822.2| ZK520.1 [Caenorhabditis elegans] 0.0
blast CAE71375 Hypothetical protein CBG18279 [Caenorhabditis briggsae] 0.0
blast XP_684320 PREDICTED: similar to WD repeat domain 19 [Danio rerio] 1.0E-114
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.24
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.2
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0012
Sequence
Target Sequence 
>C. ELEGANS:177972_AT
acacctgcgcagatgcaagctgcccaacagctgcagtccactcaatcggctgcagagaaa
ccgaccatagttgccgcggagcccatcaatcgcgtcgagtacctctccacggtcacaaac
atccagctaaactacatgtacgctgctgtcaactttgggagcaggctccgcctgcatcga
atccgaaattccgaggataacgtatcaattgagtttcccgaagcgaaccgcaacgcgacg
ctctactcttacgctttgaccgaaaattttctgatttttacgacgagctacaaaggagca
atatgggagacatttacaatcgacaaaaacacctttgccgtatttgactctcagaatatt
tacgtgtttcttctgagcaaacagcatattcaaggagaatctgtgatctacgttagcgcg
acacgcctacctcacgcctatgttcccttgtcactgaataagggaatagtaacatgtctc
atgtcaaatggaaaactctcttcggtacttttggactctcata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACACCTGCGCAGATGCAAGCTGCCC 566 109 1315 Antisense
TGCAGTCCACTCAATCGGCTGCAGA 533 577 1346 Antisense
GAAACCGACCATAGTTGCCGCGGAG 697 357 1371 Antisense
TCCACGGTCACAAACATCCAGCTAA 221 589 1420 Antisense
CATGTACGCTGCTGTCAACTTTGGG 30 211 1449 Antisense
TCCGCCTGCATCGAATCCGAAATTC 91 597 1481 Antisense
TCTACTCTTACGCTTTGACCGAAAA 121 611 1556 Antisense
CCTTTGCCGTATTTGACTCTCAGAA 268 269 1646 Antisense
TGTGATCTACGTTAGCGCGACACGC 384 553 1716 Antisense
CCTATGTTCCCTTGTCACTGAATAA 381 269 1751 Antisense
TCTTCGGTACTTTTGGACTCTCATA 652 615 1813 Antisense
Sequence Source Affymetrix Proprietary Database