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[177965_at]

Description ZK520.1 /REP_DB=WormBase Gene ID /WP=CE16726 /TR=O46018 /GB=CAB07299.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 38      4grp_total > 23      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 97.2
(3)
A 		11.1
(-3)
A 		24.5
(-2.1)
A 		94.9
(2.8)
A 		86
B 87.5
(3)
A 		16.6
(-3)
A 		29.2
(-1.9)
A 		64.9
(1.1)
A 		71
C 103.5
(2.6)
A 		35.5
(-1.9)
A 		18.6
(-3)
A 		109.8
(3)
P 		91
avr 96.1
(3) 		21.1
(-3) 		24.1
(-2.8) 		89.9
(2.5) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 45.3
(-2)
A 		78.4
(3)
A 		38.8
(-3)
A 		45.4
(-2)
A 		61
(0.4)
A 		63.2
(0.7)
A 		40
B 46.4
(1.9)
A 		20.8
(-1.6)
A 		24.3
(-1.2)
A 		10.8
(-3)
A 		46.1
(1.8)
A 		54.6
(3)
A 		44
C 116.1
(3)
A 		47.7
(-0.9)
A 		10.1
(-3)
A 		28.3
(-2)
A 		15.8
(-2.7)
A 		39.1
(-1.4)
A 		106
avr 69.3
(3) 		49
(0.3) 		24.4
(-3) 		28.2
(-2.5) 		41
(-0.8) 		52.3
(0.7) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 35.4
(3)
A 		2.4
(-3)
A 		6.4
(-2.3)
A 		13.9
(-0.9)
A 		33
B 27.9
(3)
A 		4.1
(-3)
A 		6.8
(-2.3)
A 		13.8
(-0.6)
A 		24
C 34.3
(3)
A 		10.2
(-2.2)
A 		6.5
(-3)
A 		18.5
(-0.4)
P 		28
avr 32.5
(3) 		5.6
(-3) 		6.6
(-2.8) 		15.4
(-0.8) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 8.4
(-3)
A 		27.9
(3)
A 		8.4
(-3)
A 		11.5
(-2)
A 		18.4
(0.1)
A 		15.4
(-0.8)
A 		20
B 16.1
(1.5)
A 		6.9
(-1.6)
A 		5.9
(-2)
A 		2.9
(-3)
A 		18.7
(2.4)
A 		20.4
(3)
A 		18
C 43
(3)
A 		14.6
(-1.2)
A 		2.6
(-3)
A 		5.7
(-2.5)
A 		5.1
(-2.6)
A 		13.1
(-1.4)
A 		40
avr 22.5
(3) 		16.5
(0.9) 		5.6
(-3) 		6.7
(-2.6) 		14.1
(0) 		16.3
(0.8) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177965_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7115
Sequence Type Exemplar sequence
Representative Public ID ZK520.1 NCBI
Target Description ZK520.1 /REP_DB=WormBase Gene ID /WP=CE16726 /TR=O46018 /GB=CAB07299.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067421(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067421 NCBI Caenorhabditis elegans ZK520.1 (ZK520.1) mRNA, complete cds. 10/11 None
GENEFINDER00000038081 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13675734:13678816:-1 11/11 None
SNAP00000038071 ENSEMBL cdna:SNAP chromosome:CEL140:III:13675769:13684457:-1 10/11 None
ZK520.1 ENSEMBL cdna:known chromosome:CEL140:III:13674433:13678869:-1 gene:ZK520.1 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13675729-13678748(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK520.1
Entrez Gene 176804 Entrez gene
SwissProt O46018 EMBL-EBI
WormBase CE33907 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.24727.1.S1_S_AT similar to WD repeat domain 19 cfa
DROSOPHILA_2:1628733_AT dm
DROSGENOME1:147501_AT dm
CHICKEN:GGAAFFX.8927.1.S1_S_AT similar to WD repeat membrane protein gga
CHICKEN:GGAAFFX.8927.2.S1_S_AT similar to WD repeat membrane protein gga
HG-U133_PLUS_2:220917_S_AT WD repeat domain 19 hs
HG-U133A:220917_S_AT WD repeat domain 19 hs
HG-U133A_2:220917_S_AT WD repeat domain 19 hs
HU35KSUBA:RC_AA430208_AT WD repeat domain 19 hs
U133_X3P:G13386465_3P_A_AT WD repeat domain 19 hs
U133_X3P:HS2.288821.2.A1_3P_X_AT WD repeat domain 19 hs
U133_X3P:HS2.288821.2.A1_3P_AT WD repeat domain 19 hs
U133_X3P:HS.288821.2.S1_3P_AT WD repeat domain 19 hs
U133_X3P:232163_3P_AT WD repeat domain 19 hs
U133_X3P:1555314_3P_AT WD repeat domain 19 hs
HG-U133_PLUS_2:1555314_AT WD repeat domain 19 hs
HG-U133_PLUS_2:232163_AT WD repeat domain 19 hs
HG-U133B:232163_AT WD repeat domain 19 hs
HG-U95B:56146_AT WD repeat domain 19 hs
MG-U74BV2:112005_AT WD repeat domain 19 mm
MOE430B:1455669_AT WD repeat domain 19 mm
MOUSE430_2:1455669_AT WD repeat domain 19 mm
MOE430B:1459034_AT WD repeat domain 19 mm
MOUSE430_2:1459034_AT WD repeat domain 19 mm
RG-U34C:RC_AI112384_AT similar to WD repeat membrane protein (predicted) rn
RAE230A:1390219_AT similar to WD repeat membrane protein (predicted) rn
RAT230_2:1390219_AT similar to WD repeat membrane protein (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAB07299 Hypothetical protein ZK520.1 [Caenorhabditis elegans] emb|CAD90189.1| Hypothetical protein ZK520.1 [Caenorhabditis elegans] ref|NP_499822.2| ZK520.1 [Caenorhabditis elegans] 0.0
blast CAE71375 Hypothetical protein CBG18279 [Caenorhabditis briggsae] 0.0
blast CAE71377 Hypothetical protein CBG18281 [Caenorhabditis briggsae] 0.0
blast XP_684320 PREDICTED: similar to WD repeat domain 19 [Danio rerio] 1.0E-168
Sequence
Target Sequence 
>C. ELEGANS:177965_AT
ggaactcgccgaatattacaacagagtcaatgatatgttcatggcagcgaaattctacac
acaagccggccaatacaataatgcaataaatcttctgttcaaaaatggagatgatgaaaa
ctgtgtggctcttgcagtcgactgtgggattaaatcaaaggataagacactgaataataa
gcttgtgaagtttctactcggggaagatggaaacgtgaaagatcccgcccaactgttcag
gctttacgttgggcttggaaggacaaaagacgctgctcaaactgctgtagttgttgcaca
aatacatcaggctaagggaaactatcgaatcgccagagatctcttgttccagatgcatca
gcagttgcgggagaagatgatgagaattccgttggatatgaacaaaagtttgatggcgat
acactcttatattatagtgaaagcgttgatcaatcggaaggagactttgttggctgccag
gctgcttattagaacttgtggagagatacagaggttcccgacgcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAACTCGCCGAATATTACAACAGA 204 529 1014 Antisense
TTCTACACACAAGCCGGCCAATACA 50 691 1066 Antisense
TGGCTCTTGCAGTCGACTGTGGGAT 279 545 1139 Antisense
TGGAAACGTGAAAGATCCCGCCCAA 469 547 1221 Antisense
CCGCCCAACTGTTCAGGCTTTACGT 655 261 1238 Antisense
AAGACGCTGCTCAAACTGCTGTAGT 32 155 1280 Antisense
GAATCGCCAGAGATCTCTTGTTCCA 695 329 1340 Antisense
GTTCCAGATGCATCAGCAGTTGCGG 157 441 1359 Antisense
GGAAGGAGACTTTGTTGGCTGCCAG 82 531 1469 Antisense
GCTGCCAGGCTGCTTATTAGAACTT 20 299 1486 Antisense
AGAGATACAGAGGTTCCCGACGCGT 89 69 1515 Antisense
Sequence Source Affymetrix Proprietary Database