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[177953_s_at]

Description K09A9.3 /REP_DB=WormBase Gene ID /WP=CE11976 /TR=Q93871 /GB=CAB01882.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 59      4grp_highest_8_8 > 49      4grp_high_8_8 > 42      6grp_highest_nonorm > 84      6grp_total > 49      4grp_total > 54      6grp_highest_sub > Cu      6grp_cgc > 08      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 524
1 2 3 4 range
A 883.7
(3)
P 		582.7
(-0.6)
P 		576.2
(-0.7)
P 		379.1
(-3)
P 		505
B 881.4
(3)
P 		588
(-0.4)
P 		512.4
(-1.2)
P 		359.5
(-3)
P 		522
C 822.5
(3)
P 		718
(1.5)
P 		603.7
(-0.2)
P 		416
(-3)
P 		407
avr 862.5
(3) 		629.6
(0.1) 		564.1
(-0.7) 		384.9
(-3) 		478

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 386
1 2 3 4 5 6 range
A 603.1
(1.5)
P 		520.2
(0.1)
P 		371.5
(-2.4)
P 		337.5
(-3)
P 		634.9
(2.1)
P 		689.7
(3)
P 		352
B 723.1
(3)
P 		556
(0.3)
P 		352.5
(-3)
P 		412.1
(-2)
P 		548.1
(0.2)
P 		612.4
(1.2)
P 		371
C 714.6
(3)
P 		526.4
(-0.3)
P 		376.9
(-3)
P 		378.6
(-3)
P 		622.4
(1.4)
P 		639.2
(1.7)
P 		338
avr 680.3
(3) 		534.2
(0.2) 		367
(-3) 		376.1
(-2.8) 		601.8
(1.5) 		647.1
(2.4) 		313
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 266
1 2 3 4 range
A 321.8
(3)
P 		126.5
(-1.4)
P 		150.6
(-0.9)
P 		55.5
(-3)
P 		266
B 281.1
(3)
P 		145.2
(-1)
P 		118.7
(-1.8)
P 		76.6
(-3)
P 		205
C 272.3
(3)
P 		206.4
(1)
P 		210.4
(1.2)
P 		70.1
(-3)
P 		202
avr 291.7
(3) 		159.4
(-0.5) 		159.9
(-0.5) 		67.4
(-3) 		224

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 189
1 2 3 4 5 6 range
A 111.2
(-1.4)
P 		185
(2.6)
P 		80.9
(-3)
P 		85.6
(-2.7)
P 		191.6
(3)
P 		167.9
(1.7)
P 		111
B 251.6
(3)
P 		185.1
(0.6)
P 		85.6
(-3)
P 		109.1
(-2.2)
P 		222.7
(2)
P 		228.4
(2.2)
P 		166
C 264.9
(3)
P 		160.6
(-0.3)
P 		95.9
(-2.4)
P 		76.1
(-3)
P 		201.4
(1)
P 		214
(1.4)
P 		189
avr 209.2
(3) 		176.9
(1.4) 		87.5
(-3) 		90.3
(-2.9) 		205.2
(2.8) 		203.4
(2.7) 		122
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177953_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17454
Sequence Type Exemplar sequence
Representative Public ID K09A9.3 NCBI
Target Description K09A9.3 /REP_DB=WormBase Gene ID /WP=CE11976 /TR=Q93871 /GB=CAB01882.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078170(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078170 NCBI Caenorhabditis elegans Equilibrative Nucleoside Transporter family member (ent-2) (ent-2) mRNA, complete cds. 11/11 None
SNAP00000037623 ENSEMBL cdna:SNAP chromosome:CEL140:X:15600991:15603242:1 11/11 None
GENEFINDER00000037627 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:15600991:15603417:1 11/11 None
K09A9.3.2 ENSEMBL cdna:known chromosome:CEL140:X:15600983:15603242:1 gene:K09A9.3 11/11 None
K09A9.3.1 ENSEMBL cdna:known chromosome:CEL140:X:15600985:15603681:1 gene:K09A9.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:15600990-15603242(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ent-2
Ensembl K09A9.3
Entrez Gene 181648 Entrez gene
SwissProt Q93871 EMBL-EBI
WormBase CE11976 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5337 nucleoside transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01882 Hypothetical protein K09A9.3 [Caenorhabditis elegans] ref|NP_510571.1| Equilibrative Nucleoside Transporter family member (ent-2) [Caenorhabditis elegans] 0.0
blast CAA92642 Hypothetical protein ZK809.4 [Caenorhabditis elegans] ref|NP_502204.2| Equilibrative Nucleoside Transporter family member (ent-1) [Caenorhabditis elegans] gb|AAM46663.1| equilibrative nucleoside transporter 1 [Caenorhabditis elegans] 0.0
blast CAE63332 Hypothetical protein CBG07732 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002259 EMBL-EBI Delayed-early response protein/equilibrative nucleoside transporter 1.0E-126
Pfam IPR002259 EMBL-EBI Delayed-early response protein/equilibrative nucleoside transporter 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510571.1 11 34-56,91-113,120-142,147-169,182-204,219-238,278-300,320-342,355-372,387-409,422-444
K09A9.3.2 11 34-56,91-113,120-142,147-169,182-204,219-238,278-300,320-342,355-372,387-409,422-444
K09A9.3.1 11 34-56,91-113,120-142,147-169,182-204,219-238,278-300,320-342,355-372,387-409,422-444
SNAP00000037623 10 34-56,122-144,159-181,201-223,233-255,295-317,337-359,372-389,404-426,439-461
GENEFINDER00000037627 12 34-56,91-113,120-142,147-169,182-204,219-238,278-300,320-342,355-372,387-409,422-444,459-476
Sequence
Target Sequence 
>C. ELEGANS:177953_S_AT
ctaccagttttacagcacacgtgccgaaagacaacgcgccaagaatgaggaagcagctga
caacgaaggaaagatggcaaactacattgcgaccttcaaagaggctttcccacaacttat
taatgtatttttggtgtttttcgtcacgctttcaatcttccctggagtcatgatgtacgt
taaggatgagaagaaaggaggaacctacgatttcccattgccacagaactacttcatgga
tgttaccacattccttcaattcaacgtgtttgccttcatcggatcaatcgtcgccgggag
aaagcaatggcccgccccgaacaagctctggatcccagtctacctccgtctcctctacat
cccattcttcatcttctgcaactacctccctgagacccgcacactcccggtcttcttcga
atccacgtggcttttcatcattgtggccgcatcgatgagcttcggaagtggatacttctc
aggactctccatgatgtacacttcaaagactgtcgatccatcaaaggctcaggtggctgg
aatgatggctggatttttcctcatttcaggaatcgtctcaggtctcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTACCAGTTTTACAGCACACGTGCC 293 225 741 Antisense
TCAAAGAGGCTTTCCCACAACTTAT 542 627 836 Antisense
GTTTTTCGTCACGCTTTCAATCTTC 300 449 876 Antisense
TCTTCCCTGGAGTCATGATGTACGT 682 617 896 Antisense
TCAACGTGTTTGCCTTCATCGGATC 285 631 1001 Antisense
TCATCGGATCAATCGTCGCCGGGAG 304 621 1016 Antisense
GTGGCTTTTCATCATTGTGGCCGCA 623 491 1167 Antisense
TGGCCGCATCGATGAGCTTCGGAAG 217 545 1184 Antisense
AAGTGGATACTTCTCAGGACTCTCC 578 159 1206 Antisense
AGGACTCTCCATGATGTACACTTCA 572 55 1221 Antisense
TTTCAGGAATCGTCTCAGGTCTCAT 519 671 1304 Antisense
Sequence Source Affymetrix Proprietary Database