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[177932_at]

Description T20B3.4 /REP_DB=WormBase Gene ID /WP=CE20089 /TR=Q9XUN4 /GB=CAB04742.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 02      6grp_cgc > 59      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 9.6
(-3)
A 		10.1
(-3)
A 		65.7
(0.7)
A 		101.1
(3)
A 		92
B 48.9
(0.5)
A 		20.6
(-3)
A 		28.5
(-2)
A 		69.6
(3)
A 		49
C 47.8
(1.3)
A 		60.9
(2.7)
A 		8
(-3)
A 		64.1
(3)
A 		56
avr 35.4
(-2.4) 		30.5
(-3) 		34.1
(-2.5) 		78.3
(3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 4.8
(-2.6)
A 		5.1
(-2.6)
A 		9.6
(-1.8)
A 		8.4
(-2)
A 		2.6
(-3)
A 		37
(3)
A 		34
B 12.3
(-1.5)
A 		7.7
(-2.4)
A 		36.1
(3)
A 		4.4
(-3)
A 		25.6
(1)
A 		30.4
(1.9)
A 		32
C 42.7
(1.9)
A 		15.7
(-1.5)
A 		16.4
(-1.4)
A 		3.4
(-3)
A 		51.6
(3)
A 		34.3
(0.8)
A 		48
avr 19.9
(0.1) 		9.5
(-2.1) 		20.7
(0.2) 		5.4
(-3) 		26.6
(1.5) 		33.9
(3) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 3.5
(-2.5)
A 		2.2
(-3)
A 		17.2
(3)
A 		14.8
(2)
A 		15
B 15.6
(3)
A 		5.1
(-3)
A 		6.6
(-2.1)
A 		14.8
(2.5)
A 		11
C 15.8
(2.3)
A 		17.5
(3)
A 		2.8
(-3)
A 		10.8
(0.3)
A 		15
avr 11.6
(0.8) 		8.3
(-3) 		8.9
(-2.3) 		13.5
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 0.9
(-2.9)
A 		1.8
(-2.3)
A 		2.1
(-2)
A 		2.1
(-2)
A 		0.8
(-3)
A 		9
(3)
A 		8
B 4.3
(-1.2)
A 		2.6
(-2.2)
A 		8.8
(1.5)
A 		1.2
(-3)
A 		10.4
(2.5)
A 		11.3
(3)
A 		10
C 15.8
(2.7)
A 		4.8
(-1.5)
A 		4.2
(-1.7)
A 		0.7
(-3)
A 		16.7
(3)
A 		11.5
(1.1)
A 		16
avr 7
(0.7) 		3.1
(-1.8) 		5
(-0.6) 		1.3
(-3) 		9.3
(2.2) 		10.6
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177932_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13853
Sequence Type Exemplar sequence
Representative Public ID T20B3.4 NCBI
Target Description T20B3.4 /REP_DB=WormBase Gene ID /WP=CE20089 /TR=Q9XUN4 /GB=CAB04742.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074846(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074846 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-214) (srh-214) mRNA, complete cds. 11/11 None
SNAP00000028347 ENSEMBL cdna:SNAP chromosome:CEL140:V:16830816:16832336:-1 10/11 None
GENEFINDER00000028358 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16830816:16834602:-1 11/11 None
T20B3.4 ENSEMBL cdna:known chromosome:CEL140:V:16830816:16832336:-1 gene:T20B3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16830815-16832336(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srh-214
Ensembl T20B3.4
Entrez Gene 188621 Entrez gene
SwissProt Q9XUN4 EMBL-EBI
WormBase CE20089 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04742 Hypothetical protein T20B3.4 [Caenorhabditis elegans] ref|NP_507247.1| Serpentine Receptor, class H family member (srh-214) [Caenorhabditis elegans] 1.0E-164
blast CAB04741 Hypothetical protein T20B3.3 [Caenorhabditis elegans] ref|NP_507248.1| Serpentine Receptor, class H family member (srh-215) [Caenorhabditis elegans] 1.0E-164
blast CAB04742 Hypothetical protein T20B3.4 [Caenorhabditis elegans] ref|NP_507247.1| Serpentine Receptor, class H family member (srh-214) [Caenorhabditis elegans] 1.0E-162
blast CAB04742 Hypothetical protein T20B3.4 [Caenorhabditis elegans] ref|NP_507247.1| Serpentine Receptor, class H family member (srh-214) [Caenorhabditis elegans] 1.0E-124
blast CAB04743 Hypothetical protein T20B3.5 [Caenorhabditis elegans] ref|NP_507246.1| Serpentine Receptor, class H family member (srh-216) [Caenorhabditis elegans] 1.0E-119
blast CAB04743 Hypothetical protein T20B3.5 [Caenorhabditis elegans] ref|NP_507246.1| Serpentine Receptor, class H family member (srh-216) [Caenorhabditis elegans] 1.0E-103
blast CAB04741 Hypothetical protein T20B3.3 [Caenorhabditis elegans] ref|NP_507248.1| Serpentine Receptor, class H family member (srh-215) [Caenorhabditis elegans] 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 2.5E-125
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507247.1 7 20-42,55-77,97-119,140-159,193-215,242-264,279-298
T20B3.4 7 20-42,55-77,97-119,140-159,193-215,242-264,279-298
SNAP00000028347 7 20-42,55-77,97-119,140-159,193-215,244-266,281-300
GENEFINDER00000028358 13 20-42,63-85,100-119,140-159,193-215,244-266,276-298,311-333,343-363,384-403,437-459,486-508,523-542
Sequence
Target Sequence 
>C. ELEGANS:177932_AT
actccaatctacattctccattatatccttccaataatcttcctcccggcactggtcaac
attcccgaccagaaagccgggtacagaaatttgatggaccattttgaatgtgttccaccg
tatgtagatattaagaaagttttctatcttgcaattactaagagatattttctgggtgga
tgtgcttcatttatagttgcaatgtttgttgaggtatggttctttgcatttataacgaat
cggatgttgaagaagcaaatgacaaagacaatgtcacagaagactgtggacttgcatagg
aagtttcagagagccttcattctgcagctcctcatcccattcatcatcgtcttcttacca
atctgttatgtcgggttttcttgtttcgccgagtcagcgttccacaatcaggcattaaac
aacatcacaataattataatatcatctcatggattcttctcaacaattgccatgatagct
cttcatacaccataccgggagtatacacaacagttattcccgttttgtaaacgtctggac
ttttcgtcgtccaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTCCAATCTACATTCTCCATTATA 447 99 424 Antisense
TTATATCCTTCCAATAATCTTCCTC 499 679 444 Antisense
GGCACTGGTCAACATTCCCGACCAG 485 535 471 Antisense
CAGAGAGCCTTCATTCTGCAGCTCC 69 207 730 Antisense
TCATCGTCTTCTTACCAATCTGTTA 642 621 767 Antisense
ATCTGTTATGTCGGGTTTTCTTGTT 607 31 784 Antisense
GTTTTCTTGTTTCGCCGAGTCAGCG 273 449 798 Antisense
GTCAGCGTTCCACAATCAGGCATTA 91 465 816 Antisense
TGATAGCTCTTCATACACCATACCG 241 565 896 Antisense
ATACACAACAGTTATTCCCGTTTTG 26 23 926 Antisense
ACGTCTGGACTTTTCGTCGTCCAAT 511 93 954 Antisense
Sequence Source Affymetrix Proprietary Database