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[177913_at]

Description T20B3.5 /REP_DB=WormBase Gene ID /WP=CE20090 /TR=Q9XUN3 /GB=CAB04743.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 34      6grp_total > 01      4grp_total > 07      6grp_cgc > 53      6grp_high1_sub > 02      6grp_cgc_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 38.5
(-0.9)
P 		20.8
(-3)
A 		71.6
(3)
M 		42.9
(-0.4)
A 		51
B 63
(3)
P 		30.1
(-3)
A 		42.6
(-0.7)
A 		49.5
(0.5)
A 		33
C 63.3
(-1.5)
P 		57
(-2.2)
A 		49.2
(-3)
A 		105.8
(3)
P 		57
avr 54.9
(0.8) 		36
(-3) 		54.5
(0.7) 		66.1
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 57.1
(0)
P 		50.6
(-2)
M 		49
(-2.5)
P 		47.4
(-3)
A 		66.7
(3)
M 		49.1
(-2.5)
P 		19
B 33.6
(-3)
P 		103.9
(3)
P 		39.3
(-2.5)
A 		43.9
(-2.1)
P 		58.1
(-0.9)
A 		53
(-1.3)
P 		70
C 62.6
(3)
P 		53.6
(1)
P 		43.1
(-1.3)
P 		55.2
(1.4)
P 		41.5
(-1.6)
P 		35.1
(-3)
A 		28
avr 51.1
(-1.3) 		69.4
(3) 		43.8
(-3) 		48.8
(-1.8) 		55.4
(-0.3) 		45.7
(-2.6) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 14
(1)
P 		4.5
(-3)
A 		18.7
(3)
M 		6.3
(-2.2)
A 		14
B 20.1
(3)
P 		7.4
(-3)
A 		9.9
(-1.8)
A 		10.5
(-1.5)
A 		13
C 21
(3)
P 		16.4
(-3)
A 		17.1
(-2.1)
A 		17.8
(-1.2)
P 		5
avr 18.4
(3) 		9.4
(-3) 		15.2
(0.9) 		11.5
(-1.6) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 10.5
(-3)
P 		18
(1.7)
M 		10.7
(-2.9)
P 		12
(-2.1)
A 		20.1
(3)
M 		12
(-2.1)
P 		10
B 11.7
(-2.5)
P 		34.6
(3)
P 		9.5
(-3)
A 		11.6
(-2.5)
P 		23.6
(0.4)
A 		19.8
(-0.5)
P 		25
C 23.2
(3)
P 		16.4
(-0.3)
P 		11
(-3)
P 		11.1
(-3)
P 		13.4
(-1.8)
P 		11.8
(-2.6)
A 		12
avr 15.1
(-0.8) 		23
(3) 		10.4
(-3) 		11.6
(-2.4) 		19
(1.1) 		14.5
(-1) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177913_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13747
Sequence Type Exemplar sequence
Representative Public ID T20B3.5 NCBI
Target Description T20B3.5 /REP_DB=WormBase Gene ID /WP=CE20090 /TR=Q9XUN3 /GB=CAB04743.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074845(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074845 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-216) (srh-216) mRNA, complete cds. 11/11 None
SNAP00000028346 ENSEMBL cdna:SNAP chromosome:CEL140:V:16827871:16830109:-1 11/11 None
T20B3.5 ENSEMBL cdna:known chromosome:CEL140:V:16827871:16830109:-1 gene:T20B3.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000028361 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16827870-16830109(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srh-216
Ensembl T20B3.5
Entrez Gene 188622 Entrez gene
SwissProt Q9XUN3 EMBL-EBI
WormBase CE20090 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04743 Hypothetical protein T20B3.5 [Caenorhabditis elegans] ref|NP_507246.1| Serpentine Receptor, class H family member (srh-216) [Caenorhabditis elegans] 1.0E-167
blast CAB54325 Hypothetical protein Y113G7B.2 [Caenorhabditis elegans] ref|NP_507894.1| Serpentine Receptor, class H family member (srh-217) [Caenorhabditis elegans] 1.0E-123
blast CAB04741 Hypothetical protein T20B3.3 [Caenorhabditis elegans] ref|NP_507248.1| Serpentine Receptor, class H family member (srh-215) [Caenorhabditis elegans] 1.0E-118
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507246.1 7 20-42,63-85,100-119,132-154,193-215,242-264,274-296
T20B3.5 7 20-42,63-85,100-119,132-154,193-215,242-264,274-296
SNAP00000028346 7 20-42,63-85,100-119,132-154,193-215,242-264,274-296
Sequence
Target Sequence 
>C. ELEGANS:177913_AT
caaacttcccgatcagcagacaggcaaagctaactttctgctcaaatatggatgtgttcc
accttacgtggacctcgaacgggttttttatttaataattacaaaacgatattttctaat
cacttgtgctgtatttatatgcaccatgtttgctgaggtgtggttttttgcgttggtaac
ggatcgattgttgaagaagcagatgacgaaaactatgtctcagaagacatttgatctaca
caaaaagtttcagagagcttttatactgcagcttctcatcccattcatcattgtatttct
acccatatcttacatcggggtcacttgcatttctgaatatcacaatcaatttttcaataa
cttgacattaattataatctcatctcacggattcttctcaacaattgcaatgattgcact
acatgctccatatcgggaatttacacagaaaatattcccatgtttaaaacggttcagctc
ttcggcaaccagttcaacagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAACTTCCCGATCAGCAGACAGGC 237 191 480 Antisense
ATGTGTTCCACCTTACGTGGACCTC 5 49 531 Antisense
TGAGGTGTGGTTTTTTGCGTTGGTA 647 563 633 Antisense
GCTTTTATACTGCAGCTTCTCATCC 115 305 736 Antisense
CATCCCATTCATCATTGTATTTCTA 676 211 756 Antisense
TATTTCTACCCATATCTTACATCGG 690 661 773 Antisense
ATCGGGGTCACTTGCATTTCTGAAT 9 35 793 Antisense
TAATCTCATCTCACGGATTCTTCTC 649 635 854 Antisense
GATTGCACTACATGCTCCATATCGG 487 433 891 Antisense
TTTAAAACGGTTCAGCTCTTCGGCA 108 667 942 Antisense
GCTCTTCGGCAACCAGTTCAACAGC 62 301 956 Antisense
Sequence Source Affymetrix Proprietary Database