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[177881_at]

Description F35E2.8 /REP_DB=WormBase Gene ID /WP=CE17777 /TR=O62228 /GB=CAB04286.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 54      4grp_total > 17      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 14.6
(-2.9)
A 		13.4
(-3)
A 		24.5
(-2)
A 		80.2
(3)
A 		67
B 32.7
(-1.9)
A 		56.3
(3)
P 		27.6
(-3)
A 		52.5
(2.2)
A 		29
C 23.7
(-3)
A 		25.2
(-2.5)
A 		36.8
(1.5)
A 		41.1
(3)
M 		17
avr 23.7
(-3) 		31.6
(-1.6) 		29.6
(-2) 		57.9
(3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 15.7
(-2.3)
A 		23.6
(-1.1)
A 		49.4
(3)
P 		37.6
(1.1)
M 		11.4
(-3)
A 		19
(-1.8)
A 		38
B 10.1
(-1.6)
A 		19
(0.3)
A 		20.9
(0.7)
A 		3.4
(-3)
A 		8.9
(-1.8)
A 		31.8
(3)
P 		28
C 32
(0.6)
A 		30.4
(-0.2)
A 		34.9
(2.1)
A 		36.7
(3)
A 		35.6
(2.4)
A 		24.9
(-3)
A 		12
avr 19.3
(-2.7) 		24.3
(-0.9) 		35.1
(3) 		25.9
(-0.3) 		18.6
(-3) 		25.2
(-0.6) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 5.3
(-1.4)
A 		2.9
(-3)
A 		6.4
(-0.6)
A 		11.7
(3)
A 		9
B 10.4
(0.2)
A 		13.9
(3)
P 		6.4
(-3)
A 		11.2
(0.8)
A 		8
C 7.9
(-2)
A 		7.3
(-2.6)
A 		12.8
(3)
A 		6.9
(-3)
M 		6
avr 7.9
(-3) 		8
(-2.7) 		8.5
(-1.2) 		9.9
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 2.9
(-3)
A 		8.4
(1.2)
A 		10.8
(3)
P 		9.5
(2)
M 		3.4
(-2.6)
A 		4.6
(-1.7)
A 		8
B 3.5
(-1.6)
A 		6.3
(-0)
A 		5.1
(-0.7)
A 		0.9
(-3)
A 		3.6
(-1.5)
A 		11.8
(3)
P 		11
C 11.8
(3)
A 		9.3
(-0.4)
A 		8.9
(-1)
A 		7.4
(-3)
A 		11.5
(2.6)
A 		8.3
(-1.8)
A 		4
avr 6.1
(-2.5) 		8
(2.3) 		8.3
(3) 		5.9
(-3) 		6.2
(-2.3) 		8.2
(2.8) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177881_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2418
Sequence Type Exemplar sequence
Representative Public ID F35E2.8 NCBI
Target Description F35E2.8 /REP_DB=WormBase Gene ID /WP=CE17777 /TR=O62228 /GB=CAB04286.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060585(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060585 NCBI Caenorhabditis elegans F35E2.8 (F35E2.8) mRNA, complete cds. 11/11 None
GENEFINDER00000041140 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11727033:11727912:1 9/11 None
F35E2.8 ENSEMBL cdna:known chromosome:CEL140:I:11727033:11727912:1 gene:F35E2.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000041132 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11726963-11727843(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35E2.8
Entrez Gene 185290 Entrez gene
SwissProt O62228 EMBL-EBI
WormBase CE17777 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04286 Hypothetical protein F35E2.8 [Caenorhabditis elegans] ref|NP_492986.1| F35E2.8 [Caenorhabditis elegans] 1.0E-130
blast CAB04286 Hypothetical protein F35E2.8 [Caenorhabditis elegans] ref|NP_492986.1| F35E2.8 [Caenorhabditis elegans] 1.0E-112
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.7E-5
Sequence
Target Sequence 
>C. ELEGANS:177881_AT
tgagaatttgtgagcaccctgcagccattgcagtgtgcataaattccaaaacatctcagt
cgagattcgaattgaggcacaaccttgatctagcatttctcagaataaagtttttcctag
acattttcaatgtgacaacagctggactgttgttctgtaacccaacggagaagagtatcc
gagacactttaagcctccttgtcccaattaacccaaagtttatcagcttatcgatttctg
aaatggaatccatgtctgaaagtttattcgagtacttgttaagtatcaaggcctgtcggt
tcattgttaaattgaatagttttcacgaattaacaccaagcaattcaaatttgcttctag
attgtgtttatgtgaagctaagaaatctagctttcccagatcagaaaatgattgaagtac
tcaaaacctggaaactaggatgtaatctcaagtcgctgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAGAATTTGTGAGCACCCTGCAGC 499 569 164 Antisense
GCACCCTGCAGCCATTGCAGTGTGC 590 315 177 Antisense
AAATTCCAAAACATCTCAGTCGAGA 87 115 204 Antisense
GCACAACCTTGATCTAGCATTTCTC 345 317 240 Antisense
AAGTTTTTCCTAGACATTTTCAATG 80 1 271 Antisense
GCTGGACTGTTGTTCTGTAACCCAA 92 295 304 Antisense
ATCCGAGACACTTTAAGCCTCCTTG 706 37 340 Antisense
GTTAAGTATCAAGGCCTGTCGGTTC 252 355 441 Antisense
AAGGCCTGTCGGTTCATTGTTAAAT 190 161 451 Antisense
GAAATCTAGCTTTCCCAGATCAGAA 21 363 545 Antisense
TAGGATGTAATCTCAAGTCGCTGGA 496 651 599 Antisense
Sequence Source Affymetrix Proprietary Database