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[177878_at]

Description T20B3.6 /REP_DB=WormBase Gene ID /WP=CE20091 /TR=Q9XUP1 /GB=CAB04735.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 44      4grp_total > 0      6grp_cgc > 45      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 33.5
(-0.6)
A 		15.6
(-2.9)
A 		15.1
(-3)
A 		60.6
(3)
A 		46
B 8.1
(0.1)
A 		12.5
(3)
A 		5.2
(-1.8)
A 		3.3
(-3)
A 		9
C 6.3
(-2.3)
A 		8.6
(-1.8)
A 		28.1
(3)
A 		3.6
(-3)
A 		25
avr 16
(-0.8) 		12.2
(-3) 		16.1
(-0.7) 		22.5
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 43.7
(2.3)
A 		26.1
(-0.2)
A 		48.9
(3)
A 		6.6
(-3)
A 		6.5
(-3)
A 		37.4
(1.4)
A 		42
B 5.2
(-3)
A 		50.3
(3)
A 		46
(2.4)
A 		8.3
(-2.6)
A 		5.7
(-2.9)
A 		17.1
(-1.4)
A 		45
C 22.9
(3)
A 		8.1
(-1.3)
A 		9.3
(-1)
A 		3.8
(-2.6)
A 		19.1
(1.9)
A 		2.4
(-3)
A 		21
avr 23.9
(0.7) 		28.2
(1.6) 		34.7
(3) 		6.2
(-3) 		10.4
(-2.1) 		19
(-0.3) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 12.2
(3)
A 		3.4
(-3)
A 		3.9
(-2.7)
A 		8.9
(0.8)
A 		9
B 2.6
(1.8)
A 		3.1
(3)
A 		1.2
(-1.8)
A 		0.7
(-3)
A 		2
C 2.1
(-2)
A 		2.5
(-1.8)
A 		9.8
(3)
A 		0.6
(-3)
A 		9
avr 5.6
(3) 		3
(-3) 		5
(1.6) 		3.4
(-2.1) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 8.1
(1.3)
A 		9.3
(2.1)
A 		10.7
(3)
A 		1.7
(-3)
A 		2
(-2.8)
A 		9.1
(1.9)
A 		9
B 1.8
(-3)
A 		16.8
(3)
A 		11.2
(0.8)
A 		2.2
(-2.8)
A 		2.3
(-2.8)
A 		6.4
(-1.2)
A 		15
C 8.5
(3)
A 		2.5
(-1.7)
A 		2.4
(-1.8)
A 		0.8
(-3)
A 		6.2
(1.2)
A 		0.8
(-3)
A 		8
avr 6.1
(0.4) 		9.5
(3) 		8.1
(1.9) 		1.6
(-3) 		3.5
(-1.6) 		5.4
(-0.1) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177878_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14070
Sequence Type Exemplar sequence
Representative Public ID T20B3.6 NCBI
Target Description T20B3.6 /REP_DB=WormBase Gene ID /WP=CE20091 /TR=Q9XUP1 /GB=CAB04735.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074850
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
T20B3.7 ENSEMBL cdna:known chromosome:CEL140:V:16835897:16841652:1 gene:T20B3.7 A 
NM_074850 NCBI Caenorhabditis elegans Proline HYdroxylase family member (phy-3) (phy-3) mRNA, complete cds. None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16835741-16836033(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol phy-3
Ensembl T20B3.7
Entrez Gene 188624 Entrez gene
SwissProt Q9XUP0 EMBL-EBI
WormBase CE33834 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
19538 protein metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04736 Hypothetical protein T20B3.7 [Caenorhabditis elegans] ref|NP_507251.2| Proline HYdroxylase family member (phy-3) [Caenorhabditis elegans] 0.0
blast CAE56606 Hypothetical protein CBG24361 [Caenorhabditis briggsae] 8.0E-76
blast CAE61721 Hypothetical protein CBG05671 [Caenorhabditis briggsae] 2.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR005123 EMBL-EBI 2OG-Fe(II) oxygenase superfamily 1.2E-14
Sequence
Target Sequence 
>C. ELEGANS:177878_AT
tgctcagtacgtggaaatcgggataaaactgaagttgcattttctgccgtcgccgaaaac
catcatgatttctgtcactttccgtcaaattctgggtcttgtttctctactgtgtgccac
gtggctttacagccaatgcagacatatttggctgctcgccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCAGTACGTGGAAATCGGGATA 589 579 16 Antisense
GAAATCGGGATAAAACTGAAGTTGC 358 361 29 Antisense
CTGAAGTTGCATTTTCTGCCGTCGC 119 239 44 Antisense
CGTCGCCGAAAACCATCATGATTTC 96 247 63 Antisense
ATCATGATTTCTGTCACTTTCCGTC 602 29 77 Antisense
GTCACTTTCCGTCAAATTCTGGGTC 219 457 89 Antisense
CTTTCCGTCAAATTCTGGGTCTTGT 73 221 93 Antisense
TCAAATTCTGGGTCTTGTTTCTCTA 37 629 100 Antisense
TGTGCCACGTGGCTTTACAGCCAAT 124 557 128 Antisense
GGCTTTACAGCCAATGCAGACATAT 201 535 138 Antisense
GCAGACATATTTGGCTGCTCGCCCA 62 313 153 Antisense
Sequence Source Affymetrix Proprietary Database