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[177877_at]

Description F35E2.5 /REP_DB=WormBase Gene ID /WP=CE17774 /TR=O62223 /GB=CAB04281.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 42      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 4.8
(-3)
A 		12.9
(3)
A 		10.2
(1)
A 		12.4
(2.6)
A 		8
B 5.1
(-2.5)
A 		15.2
(3)
A 		7.8
(-1)
A 		4.2
(-3)
A 		11
C 6.9
(-1.1)
A 		8.9
(2.5)
A 		5.8
(-3)
A 		9.2
(3)
A 		3
avr 5.6
(-3) 		12.3
(3) 		7.9
(-0.9) 		8.6
(-0.3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 7.7
(-2.9)
A 		4.3
(-3)
A 		31.6
(-1.9)
A 		10.3
(-2.7)
A 		14.9
(-2.6)
A 		147
(3)
A 		143
B 6.3
(-2.8)
A 		5.4
(-2.9)
A 		4.8
(-2.9)
A 		3.9
(-3)
A 		7.1
(-2.7)
A 		66.6
(3)
A 		63
C 38.4
(-0.9)
A 		8
(-2.8)
A 		18.8
(-2.1)
A 		5.4
(-3)
A 		13.4
(-2.5)
A 		99.5
(3)
A 		94
avr 17.5
(-2.3) 		5.9
(-3) 		18.4
(-2.2) 		6.5
(-3) 		11.8
(-2.6) 		104.4
(3) 		99
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 1.7
(-3)
A 		2.8
(3)
A 		2.7
(2.5)
A 		1.8
(-2.5)
A 		1
B 1.6
(-1.6)
A 		3.8
(3)
A 		1.8
(-1.1)
A 		0.9
(-3)
A 		3
C 2.3
(1.4)
A 		2.6
(3)
A 		2
(-0.3)
A 		1.5
(-3)
A 		1
avr 1.9
(-1.2) 		3.1
(3) 		2.2
(-0.2) 		1.4
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 1.4
(-3)
A 		1.5
(-3)
A 		6.9
(-2)
A 		2.6
(-2.8)
A 		4.5
(-2.5)
A 		35.8
(3)
A 		34
B 2.2
(-2.7)
A 		1.8
(-2.8)
A 		1.2
(-2.9)
A 		1
(-3)
A 		2.9
(-2.5)
A 		24.8
(3)
A 		24
C 14.2
(-0.6)
A 		2.5
(-2.7)
A 		4.8
(-2.3)
A 		1.1
(-3)
A 		4.3
(-2.4)
A 		33.3
(3)
A 		32
avr 5.9
(-2.1) 		1.9
(-2.9) 		4.3
(-2.5) 		1.6
(-3) 		3.9
(-2.5) 		31.3
(3) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177877_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.843
Sequence Type Exemplar sequence
Representative Public ID F35E2.5 NCBI
Target Description F35E2.5 /REP_DB=WormBase Gene ID /WP=CE17774 /TR=O62223 /GB=CAB04281.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060591(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060591 NCBI Caenorhabditis elegans F35E2.5 (F35E2.5) mRNA, complete cds. 11/11 None
F35E2.5 ENSEMBL cdna:known chromosome:CEL140:I:11749737:11752073:-1 gene:F35E2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11749667-11752004(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35E2.5
Entrez Gene 185288 Entrez gene
SwissProt O62223 EMBL-EBI
WormBase CE17774 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04281 Hypothetical protein F35E2.5 [Caenorhabditis elegans] ref|NP_492992.1| F35E2.5 [Caenorhabditis elegans] 0.0
blast CAB04116 Hypothetical protein F15H9.1 [Caenorhabditis elegans] ref|NP_493073.1| F15H9.1 [Caenorhabditis elegans] 2.0E-20
blast CAE67998 Hypothetical protein CBG13608 [Caenorhabditis briggsae] 2.0E-20
Protein Domains
Database ID Description E-Value
Pfam IPR005514 EMBL-EBI Protein of unknown function DUF316 2.3E-90
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492992.1 1 5-22
F35E2.5 1 5-22
Sequence
Target Sequence 
>C. ELEGANS:177877_AT
gtcatccactcagacttcaacacatcctagtcagctcagtgttaaagccactcaacttgt
ttccactgctgagccatcaacggataccttccagtctactaaaccaattgcatctacttc
attgcctacggatattccaagtagtctggtgtctgagaactctacaacctctgcagcgac
aactctcagtgagaattctacggtgatgttcattgaccagccgagtagcatatcttctga
gaatatccaacctccagaagtttcaccagtgatccctcaacgtaccgtatatctgtcttc
aacacctatgccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCATCCACTCAGACTTCAACACAT 371 467 1296 Antisense
TAAAGCCACTCAACTTGTTTCCACT 253 641 1338 Antisense
TTTCCACTGCTGAGCCATCAACGGA 359 669 1355 Antisense
GGATACCTTCCAGTCTACTAAACCA 4 495 1377 Antisense
TTGCATCTACTTCATTGCCTACGGA 76 701 1403 Antisense
TGTCTGAGAACTCTACAACCTCTGC 610 557 1445 Antisense
CTCTGCAGCGACAACTCTCAGTGAG 43 233 1464 Antisense
ATGTTCATTGACCAGCCGAGTAGCA 424 45 1501 Antisense
TATCCAACCTCCAGAAGTTTCACCA 166 657 1539 Antisense
GATCCCTCAACGTACCGTATATCTG 289 417 1566 Antisense
TATCTGTCTTCAACACCTATGCCAG 437 657 1585 Antisense
Sequence Source Affymetrix Proprietary Database