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[177864_at]

Description E01G4.2 /REP_DB=WormBase Gene ID /WP=CE09109 /TR=O17739 /GB=CAB05714.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 38      4grp_total > 37      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 52.2
(3)
A 		40.4
(0.9)
A 		18.6
(-3)
A 		24.7
(-1.9)
A 		34
B 47.8
(0.6)
A 		17.3
(-3)
A 		46.4
(0.5)
A 		67.8
(3)
A 		51
C 58.8
(0.6)
A 		88.9
(3)
A 		20.8
(-2.4)
A 		13.3
(-3)
A 		76
avr 52.9
(3) 		48.9
(2) 		28.6
(-3) 		35.3
(-1.3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 194
1 2 3 4 5 6 range
A 41.2
(-0.8)
A 		74.9
(1.3)
A 		5.2
(-3)
A 		13.8
(-2.5)
A 		9.4
(-2.7)
A 		101.6
(3)
P 		96
B 15.1
(-2.6)
A 		51.3
(1.4)
A 		11.2
(-3)
A 		20.2
(-2)
A 		18.5
(-2.2)
A 		66.4
(3)
A 		55
C 199.3
(3)
P 		17.9
(-3)
A 		64.3
(-1.5)
A 		23.4
(-2.8)
A 		56.5
(-1.7)
A 		32.8
(-2.5)
A 		181
avr 85.2
(3) 		48
(-0.4) 		26.9
(-2.3) 		19.1
(-3) 		28.1
(-2.2) 		66.9
(1.3) 		66
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 19
(3)
A 		8.8
(-1)
A 		4.9
(-2.5)
A 		3.6
(-3)
A 		15
B 15.2
(3)
A 		4.3
(-3)
A 		10.8
(0.6)
A 		14.5
(2.6)
A 		11
C 19.5
(1.4)
A 		25.6
(3)
A 		7.3
(-1.7)
A 		2.2
(-3)
A 		23
avr 17.9
(3) 		12.9
(0.3) 		7.7
(-2.5) 		6.8
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 7.6
(-1.5)
A 		26.6
(3)
A 		1.1
(-3)
A 		3.5
(-2.4)
A 		2.8
(-2.6)
A 		24.7
(2.6)
P 		26
B 5.3
(-2.3)
A 		17.1
(0.9)
A 		2.7
(-3)
A 		5.4
(-2.3)
A 		7.5
(-1.7)
A 		24.8
(3)
A 		22
C 73.9
(3)
P 		5.5
(-2.9)
A 		16.4
(-2)
A 		4.7
(-3)
A 		18.3
(-1.8)
A 		11
(-2.5)
A 		69
avr 28.9
(3) 		16.4
(-0.1) 		6.7
(-2.5) 		4.5
(-3) 		9.5
(-1.8) 		20.2
(0.9) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177864_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3314
Sequence Type Exemplar sequence
Representative Public ID E01G4.2 NCBI
Target Description E01G4.2 /REP_DB=WormBase Gene ID /WP=CE09109 /TR=O17739 /GB=CAB05714.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064431(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064431 NCBI Caenorhabditis elegans E01G4.2 (E01G4.2) mRNA, complete cds. 11/11 None
SNAP00000016566 ENSEMBL cdna:SNAP chromosome:CEL140:II:13449365:13460538:-1 11/11 None
GENEFINDER00000016579 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:13449365:13452225:-1 11/11 None
E01G4.2 ENSEMBL cdna:known chromosome:CEL140:II:13449269:13456865:-1 gene:E01G4.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000001442 1/11 Cross Hyb Matching Probes None
GENEFINDER00000001474 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13449361-13456862(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl E01G4.2
Entrez Gene 174987 Entrez gene
SwissProt O17739 EMBL-EBI
WormBase CE09109 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05714 Hypothetical protein E01G4.2 [Caenorhabditis elegans] ref|NP_496832.1| E01G4.2 [Caenorhabditis elegans] 0.0
blast CAB05714 Hypothetical protein E01G4.2 [Caenorhabditis elegans] ref|NP_496832.1| E01G4.2 [Caenorhabditis elegans] 1.0E-157
blast CAE73346 Hypothetical protein CBG20778 [Caenorhabditis briggsae] 1.0E-136
blast CAE73346 Hypothetical protein CBG20778 [Caenorhabditis briggsae] 1.0E-112
blast CAB05714 Hypothetical protein E01G4.2 [Caenorhabditis elegans] ref|NP_496832.1| E01G4.2 [Caenorhabditis elegans] 2.0E-87
blast CAE73346 Hypothetical protein CBG20778 [Caenorhabditis briggsae] 2.0E-58
blast CAB05716 Hypothetical protein E01G4.4 [Caenorhabditis elegans] ref|NP_496833.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-27) [Caenorhabditis elegans] 3.0E-25
Sequence
Target Sequence 
>C. ELEGANS:177864_AT
gaagccgcttcgacaagatcggtgcagcgttggccgaccaaatcgaagaaattctgaaat
tccagccaaaagacgagtatatgaagtgtctgctgagagcattcaaggtcgccggcgccg
agttggacacttcagaagatctgaagcttcgtgccgatcatatattgacgattatgggag
gctatgcgaaaggatcgccgcttctcggagattcagtgaaagctcagatcttttcactta
tcgaatgccgtactcgcggctgggatcgtgcggctgctcggggacctgtgagaagttcgg
cgggagccggcggcggcggcggatgtgtggctgatcgtggggagcacgactcctcgttcg
acgactcgactgacaatgatctgacggaagaggagagacagttcttggagagccatttgg
agcaagttgagagccggagaggtgctgaggaggattatgatgagcaggagatgatgaagg
agtttgggaaatttgtcaaggaggaattgcagcaggctgagctaaaatccacgtctgaaa
tgctccaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCCGCTTCGACAAGATCGGTGC 241 345 692 Antisense
TGCAGCGTTGGCCGACCAAATCGAA 291 577 714 Antisense
TGCTGAGAGCATTCAAGGTCGCCGG 277 581 782 Antisense
GCGCCGAGTTGGACACTTCAGAAGA 470 287 806 Antisense
ATCTGAAGCTTCGTGCCGATCATAT 317 33 830 Antisense
TCGCCGCTTCTCGGAGATTCAGTGA 456 599 886 Antisense
CACTTATCGAATGCCGTACTCGCGG 166 195 926 Antisense
GCTGCTCGGGGACCTGTGAGAAGTT 678 295 964 Antisense
CGACGACTCGACTGACAATGATCTG 677 251 1050 Antisense
GCAGGCTGAGCTAAAATCCACGTCT 274 311 1203 Antisense
AAATCCACGTCTGAAATGCTCCAAA 306 119 1216 Antisense
Sequence Source Affymetrix Proprietary Database