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[177853_at]

Description T22C8.1 /REP_DB=WormBase Gene ID /WP=CE13956 /TR=Q22674 /GB=CAA88873.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 37      4grp_total > 38      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 21.3
(3)
A 		9
(-1.2)
A 		15.4
(1)
A 		3.6
(-3)
A 		18
B 58.2
(3)
A 		35
(0)
A 		11.4
(-3)
A 		16.7
(-2.3)
A 		47
C 34.2
(0.8)
A 		24.7
(-0.4)
A 		2.7
(-3)
A 		52.8
(3)
A 		50
avr 37.9
(3) 		22.9
(-0.2) 		9.8
(-3) 		24.4
(0.1) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 29.9
(3)
A 		16.6
(0.1)
A 		2.8
(-2.9)
A 		2.5
(-3)
A 		17.2
(0.2)
A 		17.1
(0.2)
A 		27
B 5
(-0.3)
A 		6.3
(0.9)
A 		3.4
(-1.6)
A 		1.8
(-3)
A 		8.8
(3)
A 		3.5
(-1.5)
A 		7
C 4.3
(-2.1)
A 		14.7
(1.1)
A 		20.9
(3)
A 		3.4
(-2.4)
A 		1.4
(-3)
A 		15.1
(1.2)
A 		20
avr 13.1
(3) 		12.5
(2.7) 		9
(0.7) 		2.6
(-3) 		9.1
(0.7) 		11.9
(2.3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 7.8
(3)
A 		1.9
(-1.8)
A 		4
(-0.1)
A 		0.5
(-3)
A 		7
B 18.6
(3)
A 		8.7
(-0.7)
A 		2.6
(-3)
A 		3.6
(-2.6)
A 		16
C 11.3
(3)
A 		7.1
(0.6)
A 		1
(-3)
A 		8.9
(1.6)
A 		10
avr 12.6
(3) 		5.9
(-1) 		2.5
(-3) 		4.3
(-1.9) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 5.5
(2.5)
A 		5.9
(3)
A 		0.6
(-3)
A 		0.6
(-3)
A 		5.2
(2.2)
A 		4.2
(1.1)
A 		5
B 1.7
(-0.7)
A 		2.1
(0.1)
A 		0.8
(-2.4)
A 		0.5
(-3)
A 		3.6
(3)
A 		1.3
(-1.5)
A 		3
C 1.6
(-1.6)
A 		4.5
(2)
A 		5.3
(3)
A 		0.7
(-2.8)
A 		0.5
(-3)
A 		5.1
(2.8)
A 		5
avr 2.9
(0.8) 		4.2
(3) 		2.2
(-0.3) 		0.6
(-3) 		3.1
(1.2) 		3.5
(1.8) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177853_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3646
Sequence Type Exemplar sequence
Representative Public ID T22C8.1 NCBI
Target Description T22C8.1 /REP_DB=WormBase Gene ID /WP=CE13956 /TR=Q22674 /GB=CAA88873.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063428(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063428 NCBI Caenorhabditis elegans T22C8.1 (T22C8.1) mRNA, complete cds. 9/11 None
GENEFINDER00000014918 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8613346:8614348:1 9/11 None
T22C8.1 ENSEMBL cdna:known chromosome:CEL140:II:8613192:8614348:1 gene:T22C8.1 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000014907 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8613188-8614345(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T22C8.1
Entrez Gene 188730 Entrez gene
SwissProt Q22674 EMBL-EBI
WormBase CE35023 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA88873 Hypothetical protein T22C8.1 [Caenorhabditis elegans] ref|NP_495829.2| T22C8.1 [Caenorhabditis elegans] 1.0E-153
blast CAA88873 Hypothetical protein T22C8.1 [Caenorhabditis elegans] ref|NP_495829.2| T22C8.1 [Caenorhabditis elegans] 1.0E-132
blast CAE57558 Hypothetical protein CBG00536 [Caenorhabditis briggsae] 1.0E-128
blast CAE57558 Hypothetical protein CBG00536 [Caenorhabditis briggsae] 1.0E-110
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495829.2 5 28-50,57-79,105-127,148-170,180-202
T22C8.1 5 28-50,57-79,105-127,148-170,180-202
GENEFINDER00000014918 4 7-40,70-92,113-135,145-167
Sequence
Target Sequence 
>C. ELEGANS:177853_AT
gcttctaggcaccaatattctgacaaagtttttcacattatcatcaacttgtcttccaat
agcaatgctcggaatgtatctcgtgatttttatatttattgtcaagaagaggcaaattgc
ggataatacgaatcaaaaaaagaccgacagagacacgtcattagtggtacaagcattgat
tattacaattgcattagaggtaatactgaataattcagtcattatatcactagttagaca
cttcctcacccttttaaaaattgcatggctctttgtacaaaatcggttactagagttgca
ttgcgtcaacataactgatatcctgacacctttgttcaaaaacgctgatctttccattca
atggatttggacaattttctgttattgtacaacaatatcaaatcaacttgtaaatcctct
gttctttttgactgttaacaagatggtccgaagtgtagcaaagcacattttcgacaagaa
cattagctcgttcttatcagatacaagttccacaaataaagttccagacaggaggagaat
tcaagcaaacacaaaaaagctttgctgtggatgctgtgcgtggttgttcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTTCTAGGCACCAATATTCTGACA 36 307 297 Antisense
CACATTATCATCAACTTGTCTTCCA 204 197 330 Antisense
CTTGTCTTCCAATAGCAATGCTCGG 5 219 344 Antisense
AATGCTCGGAATGTATCTCGTGATT 72 167 360 Antisense
TATCACTAGTTAGACACTTCCTCAC 94 661 521 Antisense
GGTTACTAGAGTTGCATTGCGTCAA 307 507 581 Antisense
ACTGATATCCTGACACCTTTGTTCA 55 99 610 Antisense
AAACGCTGATCTTTCCATTCAATGG 197 127 636 Antisense
AATCCTCTGTTCTTTTTGACTGTTA 17 169 709 Antisense
ACATTAGCTCGTTCTTATCAGATAC 454 105 776 Antisense
GTGGATGCTGTGCGTGGTTGTTCAA 9 491 863 Antisense
Sequence Source Affymetrix Proprietary Database