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[177846_at]

Description E04D5.4 /REP_DB=WormBase Gene ID /WP=CE03125 /TR=Q19054 /GB=CAA91281.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 44      4grp_total > 33      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 8
(-3)
A 		84.7
(3)
A 		68.8
(1.8)
A 		61.7
(1.2)
A 		77
B 38.4
(-3)
A 		73.3
(3)
A 		54.5
(-0.2)
A 		64.6
(1.5)
A 		35
C 24.6
(-3)
A 		34.5
(-2.3)
A 		63.4
(-0.3)
A 		110
(3)
P 		85
avr 23.7
(-3) 		64.2
(1.4) 		62.2
(1.2) 		78.8
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 84.5
(-1.3)
A 		79.3
(-1.7)
A 		142.2
(3)
P 		64.9
(-2.8)
M 		64.9
(-2.8)
A 		62.3
(-3)
A 		80
B 56
(-0.1)
A 		59.9
(0.2)
A 		94.3
(3)
P 		19.5
(-3)
A 		63.9
(0.6)
P 		66.4
(0.8)
A 		75
C 63
(-1.3)
A 		97.3
(3)
A 		49.1
(-3)
A 		73.7
(0.1)
A 		52.7
(-2.6)
A 		62.5
(-1.3)
P 		48
avr 67.8
(-0.9) 		78.8
(0.7) 		95.2
(3) 		52.7
(-3) 		60.5
(-1.9) 		63.7
(-1.4) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 2.9
(-3)
A 		18.4
(3)
A 		18
(2.8)
A 		9
(-0.6)
A 		16
B 12.2
(-3)
A 		18.1
(3)
A 		12.6
(-2.6)
A 		13.8
(-1.4)
A 		6
C 8.2
(-3)
A 		9.9
(-2.3)
A 		22.1
(3)
A 		18.6
(1.5)
P 		14
avr 7.8
(-3) 		15.5
(1.7) 		17.6
(3) 		13.8
(0.7) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 15.6
(-2.8)
A 		28.2
(1.9)
A 		31
(3)
P 		16.5
(-2.5)
M 		19.6
(-1.3)
A 		15.2
(-3)
A 		16
B 19.5
(1.1)
A 		19.9
(1.2)
A 		22.9
(2.1)
P 		5.2
(-3)
A 		26
(3)
P 		24.7
(2.6)
A 		21
C 23.3
(0.8)
A 		29.7
(3)
A 		12.5
(-3)
A 		14.8
(-2.2)
A 		17.1
(-1.4)
A 		20.9
(-0.1)
P 		17
avr 19.5
(0.2) 		25.9
(3) 		22.1
(1.3) 		12.2
(-3) 		20.9
(0.8) 		20.3
(0.5) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177846_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3892
Sequence Type Exemplar sequence
Representative Public ID E04D5.4 NCBI
Target Description E04D5.4 /REP_DB=WormBase Gene ID /WP=CE03125 /TR=Q19054 /GB=CAA91281.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063842(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063842 NCBI Caenorhabditis elegans E04D5.4 (E04D5.4) mRNA, complete cds. 10/11 None
GENEFINDER00000023772 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10440196:10441865:1 11/11 None
E04D5.4 ENSEMBL cdna:known chromosome:CEL140:II:10440196:10442354:1 gene:E04D5.4 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10440192-10441866(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl E04D5.4
Entrez Gene 184038 Entrez gene
SwissProt Q19054 EMBL-EBI
WormBase CE37893 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAI46589 Hypothetical protein E04D5.4 [Caenorhabditis elegans] emb|CAA91281.3| Hypothetical protein E04D5.4 [Caenorhabditis elegans] ref|NP_496243.2| E04D5.4 [Caenorhabditis elegans] 1.0E-92
blast CAI46589 Hypothetical protein E04D5.4 [Caenorhabditis elegans] emb|CAA91281.3| Hypothetical protein E04D5.4 [Caenorhabditis elegans] ref|NP_496243.2| E04D5.4 [Caenorhabditis elegans] 4.0E-71
blast CAA88486 Hypothetical protein ZK673.1 [Caenorhabditis elegans] ref|NP_496244.1| ZK673.1 [Caenorhabditis elegans] sp|Q09662|YS51_CAEEL Hypothetical protein ZK673.1 in chromosome II precursor 1.0E-39
blast CAE59682 Hypothetical protein CBG03105 [Caenorhabditis briggsae] 5.0E-39
blast CAA88486 Hypothetical protein ZK673.1 [Caenorhabditis elegans] ref|NP_496244.1| ZK673.1 [Caenorhabditis elegans] sp|Q09662|YS51_CAEEL Hypothetical protein ZK673.1 in chromosome II precursor 7.0E-24
blast CAE59682 Hypothetical protein CBG03105 [Caenorhabditis briggsae] 1.0E-22
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.3E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 6.0E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.8E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 6.0E-15
Sequence
Target Sequence 
>C. ELEGANS:177846_AT
acagttcacattattcttctcgtcacattttccgtttctcacgttgttactaccgctgta
acgaaggctactggcgagactactgtcaggggagcaggtcaggatttgggtgatgtttct
agctcgttcttctatgaaacaacaacagcgtcaacttgtgcagatgatccgaacacagat
tgcacccaatatacctttttgtgctcaaacgccaagtatactccactactccagcaattc
tgtgcaaagacctgtgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGTTCACATTATTCTTCTCGTCA 81 109 31 Antisense
TCCGTTTCTCACGTTGTTACTACCG 88 595 61 Antisense
TACTACCGCTGTAACGAAGGCTACT 611 635 78 Antisense
GAAGGCTACTGGCGAGACTACTGTC 273 333 93 Antisense
GGCGAGACTACTGTCAGGGGAGCAG 291 501 103 Antisense
AGGGGAGCAGGTCAGGATTTGGGTG 155 55 118 Antisense
TTGGGTGATGTTTCTAGCTCGTTCT 160 707 136 Antisense
CTAGCTCGTTCTTCTATGAAACAAC 462 225 149 Antisense
ATGAAACAACAACAGCGTCAACTTG 417 23 164 Antisense
ACTACTCCAGCAATTCTGTGCAAAG 564 101 255 Antisense
GCAATTCTGTGCAAAGACCTGTGGA 124 323 264 Antisense
Sequence Source Affymetrix Proprietary Database