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[177844_at]

Description F44G4.6 /REP_DB=WormBase Gene ID /WP=CE02233 /TR=Q20416 /GB=CAA90122.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 13      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 40      6grp_total > 14      4grp_total > 38      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 539
1 2 3 4 range
A 670.1
(3)
P 		367.2
(-2.1)
P 		315.1
(-3)
P 		470.8
(-0.4)
P 		355
B 851.2
(3)
P 		521.1
(-0.8)
P 		335.5
(-3)
P 		348.2
(-2.9)
P 		516
C 854.1
(3)
P 		504.7
(-1.1)
P 		345.4
(-3)
P 		452
(-1.7)
P 		509
avr 791.8
(3) 		464.3
(-1.3) 		332
(-3) 		423.7
(-1.8) 		460

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1065
1 2 3 4 5 6 range
A 1209.5
(3)
P 		795.6
(0.6)
P 		713.4
(0.1)
P 		389.9
(-1.7)
P 		304.5
(-2.2)
P 		169.1
(-3)
P 		1040
B 1146.8
(3)
P 		1083.8
(2.6)
P 		751.1
(0.6)
P 		566.2
(-0.5)
P 		272.4
(-2.2)
P 		144.9
(-3)
P 		1002
C 862.8
(2.3)
P 		954
(3)
P 		513.8
(-0.3)
P 		545.4
(-0.1)
P 		230.2
(-2.5)
P 		161
(-3)
P 		793
avr 1073
(3) 		944.5
(2.2) 		659.4
(0.3) 		500.5
(-0.8) 		269
(-2.3) 		158.3
(-3) 		915
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 244
(3)
P 		79.7
(-2.6)
P 		82.4
(-2.5)
P 		68.9
(-3)
P 		175
B 271.5
(3)
P 		128.7
(-1.3)
P 		77.7
(-2.9)
P 		74.2
(-3)
P 		197
C 282.7
(3)
P 		145.1
(-1)
P 		120.4
(-1.7)
P 		76.2
(-3)
P 		207
avr 266.1
(3) 		117.8
(-1.6) 		93.5
(-2.4) 		73.1
(-3) 		193

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 358
1 2 3 4 5 6 range
A 223
(1.5)
P 		282.9
(3)
P 		155.4
(-0.2)
P 		98.9
(-1.6)
P 		91.9
(-1.7)
P 		41.2
(-3)
P 		242
B 399
(3)
P 		360.8
(2.3)
P 		182.4
(-0.8)
P 		150
(-1.3)
P 		110.7
(-2)
P 		54
(-3)
P 		345
C 319.8
(3)
P 		291.1
(2.4)
P 		130.8
(-1.3)
P 		109.7
(-1.7)
P 		74.5
(-2.5)
P 		53.9
(-3)
P 		266
avr 313.9
(3) 		311.6
(2.9) 		156.2
(-0.6) 		119.5
(-1.4) 		92.4
(-2) 		49.7
(-3) 		264
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177844_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3483
Sequence Type Exemplar sequence
Representative Public ID F44G4.6 NCBI
Target Description F44G4.6 /REP_DB=WormBase Gene ID /WP=CE02233 /TR=Q20416 /GB=CAA90122.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063522(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063522 NCBI Caenorhabditis elegans F44G4.6 (F44G4.6) mRNA, complete cds. 11/11 None
F44G4.6 ENSEMBL cdna:known chromosome:CEL140:II:9000915:9001697:-1 gene:F44G4.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000000993 5/11 Cross Hyb Matching Probes None
GENEFINDER00000001003 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9000911-9001907(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F44G4.6
Entrez Gene 185762 Entrez gene
SwissProt Q20416 EMBL-EBI
WormBase CE28558 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
5840 ribosome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3735 structural constituent of ribosome inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90122 Hypothetical protein F44G4.6 [Caenorhabditis elegans] ref|NP_495923.1| F44G4.6 [Caenorhabditis elegans] 1.0E-98
blast CAE59785 Hypothetical protein CBG03237 [Caenorhabditis briggsae] 2.0E-52
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495923.1 4 12-34,38-55,62-84,131-150
F44G4.6 4 12-34,38-55,62-84,131-150
Sequence
Target Sequence 
>C. ELEGANS:177844_AT
gaagatgcctttccattattcactttctctgtggcttcttcatacttatgtctggagttg
gctcaaactatttgtcccatgatccattttcaatgagttttgcttttatctacattttgg
tatctcttatatgttgtgtgacaactagaaactacgatccactttgtctccgaatgtgcg
catttttatcatttgtgatgacagttgcatcagcctatctcgtctatcaacatgctagtc
agacagaacaagtttgcctacgcaaatgcgttcgaactgaggactactttcacacacaac
tagcaatcatttgtgggatacaaggtttcaaattaccaatgggaagtccgataagttgtc
aacgatttatctcatttttagttttaaactgcgtcatctctattgtctattcgtgtctgt
gcttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGATGCCTTTCCATTATTCACTT 163 339 101 Antisense
ATTATTCACTTTCTCTGTGGCTTCT 595 13 115 Antisense
TCTGTGGCTTCTTCATACTTATGTC 58 609 128 Antisense
AACTATTTGTCCCATGATCCATTTT 686 139 166 Antisense
TAGAAACTACGATCCACTTTGTCTC 251 651 246 Antisense
TCCACTTTGTCTCCGAATGTGCGCA 353 589 258 Antisense
GATGACAGTTGCATCAGCCTATCTC 91 413 297 Antisense
AGCCTATCTCGTCTATCAACATGCT 663 83 312 Antisense
AGGACTACTTTCACACACAACTAGC 604 47 380 Antisense
AAACTGCGTCATCTCTATTGTCTAT 432 127 486 Antisense
TATTGTCTATTCGTGTCTGTGCTTG 186 665 501 Antisense
Sequence Source Affymetrix Proprietary Database