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[177837_at]

Description M04D8.4 /REP_DB=WormBase Gene ID /WP=CE00622 /TR=Q21509 /GB=CAA83612.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 08      6grp_total > 08      4grp_total > 34      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 110
1 2 3 4 range
A 55.3
(-3)
A 		80.8
(0.1)
A 		81.2
(0.1)
A 		104.7
(3)
A 		49
B 60
(-0.4)
A 		83.9
(3)
P 		41.2
(-3)
A 		48.5
(-2)
A 		43
C 51
(-3)
P 		150.9
(3)
P 		88.4
(-0.8)
P 		83.9
(-1)
A 		100
avr 55.4
(-3) 		105.2
(3) 		70.3
(-1.2) 		79
(-0.2) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 85.6
(3)
P 		51.3
(-3)
A 		65
(-0.6)
P 		62.9
(-1)
M 		68.8
(0.1)
P 		64
(-0.8)
P 		34
B 65.3
(2.9)
P 		65.8
(3)
P 		44.5
(-3)
P 		47.8
(-2.1)
P 		55.5
(0.1)
A 		49.8
(-1.5)
P 		21
C 97.5
(3)
P 		68.8
(0.5)
P 		59.8
(-0.3)
A 		91.6
(2.5)
P 		29.9
(-3)
A 		55.4
(-0.7)
P 		68
avr 82.8
(3) 		62
(-1) 		56.4
(-2) 		67.4
(0.1) 		51.4
(-3) 		56.4
(-2) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 20.2
(2)
A 		17.5
(-0.8)
A 		21.2
(3)
A 		15.3
(-3)
A 		6
B 19.1
(2.1)
A 		20.7
(3)
P 		9.5
(-3)
A 		10.3
(-2.6)
A 		11
C 16.9
(-2.4)
P 		43.4
(3)
P 		30.8
(0.4)
P 		14.2
(-3)
A 		29
avr 18.7
(-0.7) 		27.2
(3) 		20.5
(0.1) 		13.3
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 15.8
(-1.5)
P 		18.2
(0.6)
A 		14.2
(-3)
P 		15.9
(-1.5)
M 		20.8
(3)
P 		15.6
(-1.7)
P 		7
B 22.7
(3)
P 		21.9
(2.6)
P 		10.8
(-3)
P 		12.7
(-2)
P 		22.6
(2.9)
A 		18.6
(0.9)
P 		12
C 36.1
(3)
P 		21
(-0.4)
P 		15.2
(-1.8)
A 		18.4
(-1)
P 		9.7
(-3)
A 		18.5
(-1)
P 		26
avr 24.9
(3) 		20.4
(0.7) 		13.4
(-3) 		15.7
(-1.8) 		17.7
(-0.8) 		17.6
(-0.8) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177837_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8066
Sequence Type Exemplar sequence
Representative Public ID M04D8.4 NCBI
Target Description M04D8.4 /REP_DB=WormBase Gene ID /WP=CE00622 /TR=Q21509 /GB=CAA83612.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066824(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066824 NCBI Caenorhabditis elegans M04D8.4 (M04D8.4) mRNA, complete cds. 11/11 None
GENEFINDER00000022950 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10029883:10031015:-1 11/11 None
M04D8.4 ENSEMBL cdna:known chromosome:CEL140:III:10029883:10031015:-1 gene:M04D8.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000022943 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10029878-10031011(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M04D8.4
Entrez Gene 187443 Entrez gene
SwissProt Q21509 EMBL-EBI
WormBase CE00622 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83612 Hypothetical protein M04D8.4 [Caenorhabditis elegans] ref|NP_499225.1| M04D8.4 [Caenorhabditis elegans] 1.0E-123
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499225.1 2 89-108,141-163
M04D8.4 2 89-108,141-163
GENEFINDER00000022950 2 89-108,141-163
Sequence
Target Sequence 
>C. ELEGANS:177837_AT
attcctcaataacatctgggtccgattgatgaactacatccaggtgaattatttttggtt
gggcctcaacgaaaactcccttttcgcggagcatttctcaacagctgtactatccgctat
taatgcatcagacatctccttgatcgtcactgattcgtttacctactttaactttgtaca
aaaagatattcctgtctctgatagagtcaccgataaaattgtcatactgtgctacatcgc
tgttccagtttacatatattttcgagctgttcacattttttgtcactataaaataattcg
agacgttcatcaccgtaccattctcgggacgaatggaacacaaattcttgtcaaaagtct
catcggaagccgagtttacatcgccatcttcgggtccttcttcataaattctgtatttgg
gtttttgattggaaacccatggaaagggattgctgcttctctactacactttggtggcaa
tgtatttctggtaaaaaaccaatgtgaaatcgcacaacttgcaatcgaaatgaaacgtcg
tatgcagccgcacaaggaaccatcaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCCTCAATAACATCTGGGTCCGA 471 7 60 Antisense
CTCAACAGCTGTACTATCCGCTATT 96 229 156 Antisense
TCAGACATCTCCTTGATCGTCACTG 662 623 187 Antisense
ATCGTCACTGATTCGTTTACCTACT 549 35 202 Antisense
TACTGTGCTACATCGCTGTTCCAGT 297 647 284 Antisense
GAGCTGTTCACATTTTTTGTCACTA 540 387 323 Antisense
ATTCGAGACGTTCATCACCGTACCA 706 7 355 Antisense
CATCTTCGGGTCCTTCTTCATAAAT 388 213 444 Antisense
GGAAAGGGATTGCTGCTTCTCTACT 241 527 500 Antisense
TGCTTCTCTACTACACTTTGGTGGC 523 577 513 Antisense
TGCAGCCGCACAAGGAACCATCAGA 20 573 602 Antisense
Sequence Source Affymetrix Proprietary Database