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[177833_s_at]

Description F19B2.5 /REP_DB=WormBase Gene ID /WP=CE20697 /TR=Q9XXT0 /GB=CAA16269.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 58      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 73      6grp_total > 69      4grp_total > 60      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 355
1 2 3 4 range
A 482.8
(0.5)
P 		407.5
(-1.5)
P 		581.3
(3)
P 		349.5
(-3)
P 		232
B 406.5
(0.7)
P 		395.1
(0.5)
P 		500
(3)
P 		252.4
(-3)
P 		248
C 471.3
(0.4)
P 		431.6
(-0.3)
P 		607.8
(3)
P 		288.6
(-3)
P 		319
avr 453.5
(0.5) 		411.4
(-0.4) 		563
(3) 		296.8
(-3) 		266

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 336
1 2 3 4 5 6 range
A 475.5
(-1.2)
P 		524.1
(0.3)
P 		560.5
(1.3)
P 		439.5
(-2.2)
P 		413.4
(-3)
P 		617.5
(3)
P 		204
B 537.8
(1)
P 		517.6
(0.5)
P 		506.9
(0.2)
P 		391.3
(-3)
P 		536
(1)
P 		609.6
(3)
P 		218
C 605.3
(1.3)
P 		530.5
(-0.1)
P 		442.5
(-1.6)
P 		365.6
(-3)
P 		625.6
(1.6)
P 		701.4
(3)
P 		336
avr 539.5
(0.5) 		524.1
(0.1) 		503.3
(-0.4) 		398.8
(-3) 		525
(0.1) 		642.8
(3) 		244
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 163
1 2 3 4 range
A 175.8
(3)
P 		88.5
(-1.2)
P 		152
(1.9)
P 		51.2
(-3)
P 		125
B 129.6
(3)
P 		97.6
(0.5)
P 		115.9
(1.9)
P 		53.8
(-3)
P 		76
C 156
(0.9)
P 		124.1
(-0.2)
P 		211.8
(3)
P 		48.7
(-3)
P 		163
avr 153.8
(2.7) 		103.4
(-0.1) 		159.9
(3) 		51.2
(-3) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 161
1 2 3 4 5 6 range
A 87.7
(-3)
P 		186.4
(3)
P 		122.1
(-0.9)
P 		111.5
(-1.6)
P 		124.8
(-0.7)
P 		150.3
(0.8)
P 		99
B 187.1
(1.1)
P 		172.3
(0.3)
P 		123.1
(-2.1)
P 		103.6
(-3)
P 		217.8
(2.5)
P 		227.3
(3)
P 		124
C 224.3
(2.6)
P 		161.9
(0.3)
P 		112.6
(-1.5)
P 		73.5
(-3)
P 		202.4
(1.8)
P 		234.8
(3)
P 		161
avr 166.4
(0.9) 		173.5
(1.3) 		119.3
(-1.7) 		96.2
(-3) 		181.7
(1.8) 		204.1
(3) 		108
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177833_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14628
Sequence Type Exemplar sequence
Representative Public ID F19B2.5 NCBI
Target Description F19B2.5 /REP_DB=WormBase Gene ID /WP=CE20697 /TR=Q9XXT0 /GB=CAA16269.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075485(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075485 NCBI Caenorhabditis elegans F19B2.5 (F19B2.5) mRNA, complete cds. 11/11 None
GENEFINDER00000019296 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20160820:20161352:-1 11/11 None
F19B2.5 ENSEMBL cdna:known chromosome:CEL140:V:20160645:20161352:-1 gene:F19B2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20160819-20161352(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F19B2.5
Entrez Gene 180319 Entrez gene
SwissProt Q9XXT0 EMBL-EBI
WormBase CE20697 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA16269 Hypothetical protein F19B2.5 [Caenorhabditis elegans] ref|NP_507886.1| F19B2.5 [Caenorhabditis elegans] 2.0E-78
blast CAB03923 Hypothetical protein C25F9.5 [Caenorhabditis elegans] ref|NP_507777.1| C25F9.5 [Caenorhabditis elegans] 9.0E-15
blast CAB07268 Hypothetical protein M04C3.1a [Caenorhabditis elegans] emb|CAB03924.1| Hypothetical protein M04C3.1a [Caenorhabditis elegans] ref|NP_507782.1| M04C3.1a [Caenorhabditis elegans] 5.0E-14
Sequence
Target Sequence 
>C. ELEGANS:177833_S_AT
taaacgatgcgggagaacttgccaaacacagcgtcgttgttacgacttttgatacgatca
aaactctgatgaactttcaaactctgcagaaaatatcattcgaaagaataattatttatg
atgcgtatccgatcgataatacggacacacaaagatacaaagctttgcgacaacttgacg
ccacctaccgctgggttctaacggggaatcttcttcaacaaaatttggcaaacttcctca
aacttggcgagtatggggacgatcaactctgggacactcaaatttgtcccattctgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAACGATGCGGGAGAACTTGCCAA 299 641 23 Antisense
AACTTGCCAAACACAGCGTCGTTGT 552 137 38 Antisense
GTTGTTACGACTTTTGATACGATCA 511 435 58 Antisense
TGATGAACTTTCAAACTCTGCAGAA 215 567 89 Antisense
ATTTATGATGCGTATCCGATCGATA 504 15 136 Antisense
TTTGCGACAACTTGACGCCACCTAC 708 661 186 Antisense
GCTGGGTTCTAACGGGGAATCTTCT 3 293 212 Antisense
TGGCAAACTTCCTCAAACTTGGCGA 684 523 248 Antisense
GTATGGGGACGATCAACTCTGGGAC 343 437 273 Antisense
GATCAACTCTGGGACACTCAAATTT 666 421 283 Antisense
ACACTCAAATTTGTCCCATTCTGGA 701 109 296 Antisense
Sequence Source Affymetrix Proprietary Database