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[177832_at]

Description K02E2.8 /REP_DB=WormBase Gene ID /WP=CE21016 /TR=Q9NAQ1 /GB=CAB76737.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 11      4grp_total > 49      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 360
1 2 3 4 range
A 11.3
(-3)
A 		371.3
(3)
P 		275.3
(1.4)
P 		77.3
(-1.9)
A 		360
B 15.8
(-3)
A 		303.5
(3)
P 		275.3
(2.4)
P 		55.1
(-2.2)
A 		288
C 56.1
(-3)
A 		334.1
(3)
P 		286.9
(2)
P 		69.9
(-2.7)
A 		278
avr 27.7
(-3) 		336.3
(3) 		279.2
(1.9) 		67.4
(-2.2) 		309

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 212
1 2 3 4 5 6 range
A 71.7
(-1.2)
A 		189.7
(3)
A 		91.1
(-0.5)
A 		105.7
(0)
A 		38.1
(-2.4)
A 		21.5
(-3)
A 		168
B 55
(-2.3)
A 		215.4
(3)
A 		82.3
(-1.4)
A 		64.2
(-2)
A 		44.5
(-2.7)
A 		34.5
(-3)
A 		181
C 3.6
(-3)
A 		179
(3)
A 		75
(-0.6)
A 		18.7
(-2.5)
A 		9.2
(-2.8)
A 		40
(-1.8)
A 		175
avr 43.4
(-2.5) 		194.7
(3) 		82.8
(-1.1) 		62.9
(-1.8) 		30.6
(-3) 		32
(-2.9) 		164
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 4.1
(-3)
A 		80.6
(3)
P 		72
(2.3)
P 		11.3
(-2.4)
A 		77
B 5
(-3)
A 		75
(3)
P 		63.8
(2)
P 		11.7
(-2.4)
A 		70
C 18.6
(-2.5)
A 		96.1
(2.7)
P 		100
(3)
P 		11.8
(-3)
A 		88
avr 9.2
(-3) 		83.9
(3) 		78.6
(2.6) 		11.6
(-2.8) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 13.2
(-2.2)
A 		67.5
(3)
A 		19.8
(-1.6)
A 		26.8
(-0.9)
A 		11.5
(-2.4)
A 		5.2
(-3)
A 		62
B 19.1
(-2.4)
A 		71.7
(3)
A 		20
(-2.3)
A 		17
(-2.6)
A 		18.1
(-2.5)
A 		12.9
(-3)
A 		59
C 1.3
(-3)
A 		54.6
(3)
A 		19.1
(-1)
A 		3.8
(-2.7)
A 		3
(-2.8)
A 		13.4
(-1.6)
A 		53
avr 11.2
(-2.9) 		64.6
(3) 		19.6
(-2) 		15.9
(-2.4) 		10.9
(-3) 		10.5
(-3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177832_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13812
Sequence Type Exemplar sequence
Representative Public ID K02E2.8 NCBI
Target Description K02E2.8 /REP_DB=WormBase Gene ID /WP=CE21016 /TR=Q9NAQ1 /GB=CAB76737.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075523(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075523 NCBI Caenorhabditis elegans K02E2.8 (K02E2.8) mRNA, complete cds. 11/11 None
GENEFINDER00000027383 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20363857:20366338:-1 9/11 None
K02E2.8 ENSEMBL cdna:known chromosome:CEL140:V:20363857:20364983:-1 gene:K02E2.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000027375 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20363856-20364983(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K02E2.8
Entrez Gene 186883 Entrez gene
SwissProt Q9NAQ1 EMBL-EBI
WormBase CE35574 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04548 Hypothetical protein K02E2.3 [Caenorhabditis elegans] ref|NP_507925.1| K02E2.3 [Caenorhabditis elegans] 1.0E-73
blast CAB76737 Hypothetical protein K02E2.8 [Caenorhabditis elegans] ref|NP_507924.2| K02E2.8 [Caenorhabditis elegans] 7.0E-71
blast CAB76737 Hypothetical protein K02E2.8 [Caenorhabditis elegans] ref|NP_507924.2| K02E2.8 [Caenorhabditis elegans] 3.0E-57
blast CAB04999 Hypothetical protein ZK228.8 [Caenorhabditis elegans] emb|CAA16412.1| Hypothetical protein ZK228.8 [Caenorhabditis elegans] ref|NP_507619.1| Serpentine Receptor, class H family member (srh-178) [Caenorhabditis elegans] 8.0E-36
blast AAK68160 Hypothetical protein C04G6.2 [Caenorhabditis elegans] ref|NP_494941.1| C04G6.2 [Caenorhabditis elegans] 3.0E-14
blast T29596 hypothetical protein C04G6.2 - Caenorhabditis elegans 3.0E-14
Protein Domains
Database ID Description E-Value
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 1.0E-8
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.2E-74
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 1.0E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000027383 3 68-90,110-132,145-167
Sequence
Target Sequence 
>C. ELEGANS:177832_AT
gaaacttgcagcggcttgcattccgacgcaatttgttactccactcaacccaggcaccat
aactactaccacgacaatttcggctccgactacaaccgtacctattactactacaaatcc
tgtatctaccactactacgactacaactacaaccacagaagccccaacaactacgacaac
tgtggtgcctatcccttgcaacatttgcacctcaccctacaatgcaggatgtcaaggaat
gggagtaccaagtcctaccgactggtgtgcgattagtggcagttttccaataatgttcag
acgatatccggaggaaacaacagcagacgctcaatgcgtctgccctgcaggcaccgccgc
ctacctactgacaattgtcggagagcttcctgcaaacttcggaggaactggaacgttcag
tgcggattgctgggacacgggaaatttcaacacacctggagtatggacttactacttttt
tgcttatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACTTGCAGCGGCTTGCATTCCG 470 355 36 Antisense
ATTCCGACGCAATTTGTTACTCCAC 351 7 55 Antisense
TCAACCCAGGCACCATAACTACTAC 595 631 80 Antisense
GACAATTTCGGCTCCGACTACAACC 445 363 108 Antisense
GACTACAACCGTACCTATTACTACT 139 359 123 Antisense
CAAATCCTGTATCTACCACTACTAC 464 193 149 Antisense
ACTACGACAACTGTGGTGCCTATCC 86 101 205 Antisense
TGCCTATCCCTTGCAACATTTGCAC 564 585 221 Antisense
AGTCCTACCGACTGGTGTGCGATTA 36 61 286 Antisense
TCGGAGAGCTTCCTGCAAACTTCGG 40 607 413 Antisense
GGACTTACTACTTTTTTGCTTATGG 336 523 500 Antisense
Sequence Source Affymetrix Proprietary Database