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[177826_at]

Description F44A6.4 /REP_DB=WormBase Gene ID /WP=CE03325 /TR=Q20386 /GB=CAA90724.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 71      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 53      6grp_total > 71      4grp_total > 49      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 459
1 2 3 4 range
A 799.4
(-2.5)
P 		870.1
(-0.7)
P 		1017.7
(3)
P 		780.1
(-3)
P 		238
B 720.5
(-3)
P 		1087.9
(2.8)
P 		1099.2
(3)
P 		817.8
(-1.5)
P 		379
C 872.9
(-1.8)
P 		1179.6
(3)
P 		1005.5
(0.3)
P 		798.1
(-3)
P 		382
avr 797.6
(-3) 		1045.9
(3) 		1040.8
(2.9) 		798.7
(-3) 		248

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1150
1 2 3 4 5 6 range
A 760
(-0.7)
P 		878.8
(0.3)
P 		1211.4
(3)
P 		1205.6
(3)
P 		818.7
(-0.2)
P 		480.6
(-3)
P 		731
B 570.6
(-3)
P 		830.5
(-0.7)
P 		1257.2
(3)
P 		952.6
(0.3)
P 		972.1
(0.5)
P 		592.1
(-2.8)
P 		687
C 559.1
(-3)
P 		735.4
(-2)
P 		734.8
(-2)
P 		1630.3
(3)
P 		984.6
(-0.6)
P 		709.6
(-2.2)
P 		1071
avr 629.9
(-2.7) 		814.9
(-1) 		1067.8
(1.3) 		1262.8
(3) 		925.1
(-0) 		594.1
(-3) 		669
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 236
1 2 3 4 range
A 291.1
(3)
P 		188.9
(-0.5)
P 		266.1
(2.2)
P 		114.2
(-3)
P 		177
B 229.8
(0.5)
P 		268.7
(3)
P 		254.7
(2.1)
P 		174.3
(-3)
P 		94
C 289
(1.3)
P 		339.1
(2.7)
P 		350.4
(3)
P 		134.6
(-3)
P 		216
avr 270
(2.2) 		265.6
(2) 		290.4
(3) 		141
(-3) 		149

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 278
1 2 3 4 5 6 range
A 140.1
(-2.3)
P 		312.5
(3)
P 		263.8
(1.5)
P 		305.8
(2.8)
P 		247
(1)
P 		117
(-3)
P 		196
B 198.6
(-3)
P 		276.4
(-0.6)
P 		305.3
(0.3)
P 		252.3
(-1.4)
P 		394.9
(3)
P 		220.8
(-2.3)
P 		196
C 207.2
(-2.1)
P 		224.4
(-1.4)
P 		187
(-3)
P 		327.9
(3)
P 		318.6
(2.6)
P 		237.5
(-0.8)
P 		141
avr 182
(-3) 		271.1
(0.9) 		252
(0) 		295.3
(1.9) 		320.2
(3) 		191.8
(-2.6) 		138
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177826_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16652
Sequence Type Exemplar sequence
Representative Public ID F44A6.4 NCBI
Target Description F44A6.4 /REP_DB=WormBase Gene ID /WP=CE03325 /TR=Q20386 /GB=CAA90724.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077315(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077315 NCBI Caenorhabditis elegans F44A6.4 (F44A6.4) mRNA, complete cds. 11/11 None
SNAP00000009274 ENSEMBL cdna:SNAP chromosome:CEL140:X:10214489:10215407:-1 11/11 None
GENEFINDER00000009279 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:10214489:10217946:-1 10/11 None
F44A6.4 ENSEMBL cdna:known chromosome:CEL140:X:10214489:10215407:-1 gene:F44A6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10214488-10215407(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F44A6.4
Entrez Gene 185724 Entrez gene
SwissProt Q20386 EMBL-EBI
WormBase CE37373 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90724 Hypothetical protein F44A6.4 [Caenorhabditis elegans] ref|NP_509716.2| F44A6.4 [Caenorhabditis elegans] 3.0E-86
blast CAA90724 Hypothetical protein F44A6.4 [Caenorhabditis elegans] ref|NP_509716.2| F44A6.4 [Caenorhabditis elegans] 2.0E-85
blast CAA90724 Hypothetical protein F44A6.4 [Caenorhabditis elegans] ref|NP_509716.2| F44A6.4 [Caenorhabditis elegans] 2.0E-74
blast CAA90739 Hypothetical protein F44A6.5 [Caenorhabditis elegans] emb|CAA90725.1| Hypothetical protein F44A6.5 [Caenorhabditis elegans] ref|NP_509717.1| F44A6.5 [Caenorhabditis elegans] 6.0E-72
blast CAE70546 Hypothetical protein CBG17187 [Caenorhabditis briggsae] 3.0E-19
Sequence
Target Sequence 
>C. ELEGANS:177826_AT
tgccaagacaccggcatgctcatgggctcgatcgttttcaggagcacctgcggctcatgt
ctgccagtgacttgtacaactttataggacaatttttactggctccagaagaggtcgttt
ggcacgcacaaggaaacttgaaaccaaattgtttcaacgacactgtcactgctcatttgt
atacttttccatacaacattctgacaaactgtatttttgcgtgtgaagattttattaata
aacaaagaaaagaatctgccgagaatcttaaacgttctcaacactttgaaaatgataaca
tgagaccagccgcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCCAAGACACCGGCATGCTCATGG 174 587 14 Antisense
CTCATGGGCTCGATCGTTTTCAGGA 333 225 32 Antisense
TTTTCAGGAGCACCTGCGGCTCATG 710 671 48 Antisense
GCTCATGTCTGCCAGTGACTTGTAC 538 301 66 Antisense
AGGACAATTTTTACTGGCTCCAGAA 161 55 99 Antisense
AGAAGAGGTCGTTTGGCACGCACAA 360 73 120 Antisense
CAACGACACTGTCACTGCTCATTTG 557 187 168 Antisense
ACTGCTCATTTGTATACTTTTCCAT 208 99 181 Antisense
ACTTTTCCATACAACATTCTGACAA 263 103 196 Antisense
GAATCTTAAACGTTCTCAACACTTT 635 321 276 Antisense
ATGATAACATGAGACCAGCCGCAAA 284 45 305 Antisense
Sequence Source Affymetrix Proprietary Database