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[177825_at]

Description F58E6.4 /REP_DB=WormBase Gene ID /WP=CE06019 /TR=Q20980 /GB=CAA94774.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 62      4grp_total > 56      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 2.4
(-3)
A 		14.9
(1.5)
A 		19.1
(3)
A 		4.4
(-2.3)
A 		17
B 3.5
(-3)
A 		5.1
(-2.4)
A 		18.6
(3)
A 		4.7
(-2.5)
A 		15
C 5.2
(-3)
A 		11.9
(-2.4)
A 		69.2
(3)
A 		5.9
(-2.9)
A 		64
avr 3.7
(-3) 		10.6
(-1.7) 		35.6
(3) 		5
(-2.8) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1829
1 2 3 4 5 6 range
A 13.4
(-3)
A 		361
(-1.9)
P 		1837.8
(3)
P 		992.7
(0.2)
P 		345.4
(-1.9)
P 		85.5
(-2.8)
A 		1824
B 16.4
(-3)
A 		389.7
(-1.6)
P 		1567.7
(3)
P 		682.7
(-0.4)
P 		430.6
(-1.4)
P 		63.1
(-2.8)
A 		1551
C 8.6
(-3)
A 		434
(-1.3)
P 		1492.2
(3)
P 		764
(0.1)
P 		432.2
(-1.3)
P 		64.8
(-2.8)
A 		1484
avr 12.8
(-3) 		394.9
(-1.6) 		1632.6
(3) 		813.1
(-0) 		402.7
(-1.6) 		71.1
(-2.8) 		1620
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 0.9
(-2.6)
A 		3.2
(0.5)
A 		5
(3)
A 		0.6
(-3)
A 		4
B 1.1
(-2.8)
A 		1.3
(-2.5)
A 		4.3
(3)
A 		1
(-3)
A 		3
C 1.7
(-2.8)
A 		3.4
(-2.4)
A 		24.1
(3)
A 		1
(-3)
A 		23
avr 1.2
(-2.8) 		2.6
(-2) 		11.1
(3) 		0.9
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 398
1 2 3 4 5 6 range
A 2.5
(-3)
A 		128.4
(-1.1)
P 		400.2
(3)
P 		251.8
(0.8)
P 		104.2
(-1.5)
P 		20.8
(-2.7)
A 		398
B 5.7
(-3)
A 		129.7
(-1)
P 		380.7
(3)
P 		180.8
(-0.2)
P 		175
(-0.3)
P 		23.5
(-2.7)
A 		375
C 3.2
(-3)
A 		132.4
(-0.9)
P 		379.8
(3)
P 		153.6
(-0.6)
P 		139.9
(-0.8)
P 		21.7
(-2.7)
A 		377
avr 3.8
(-3) 		130.2
(-1) 		386.9
(3) 		195.4
(0) 		139.7
(-0.9) 		22
(-2.7) 		383
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177825_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11430
Sequence Type Exemplar sequence
Representative Public ID F58E6.4 NCBI
Target Description F58E6.4 /REP_DB=WormBase Gene ID /WP=CE06019 /TR=Q20980 /GB=CAA94774.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073113(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073113 NCBI Caenorhabditis elegans F58E6.4 (F58E6.4) mRNA, complete cds. 11/11 None
SNAP00000040366 ENSEMBL cdna:SNAP chromosome:CEL140:V:9752553:9752990:-1 11/11 None
GENEFINDER00000040371 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9752553:9752951:-1 11/11 None
F58E6.4 ENSEMBL cdna:known chromosome:CEL140:V:9752553:9752990:-1 gene:F58E6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9752552-9752990(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58E6.4
Entrez Gene 186524 Entrez gene
SwissProt Q20980 EMBL-EBI
WormBase CE06019 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94774 Hypothetical protein F58E6.4 [Caenorhabditis elegans] ref|NP_505514.1| F58E6.4 [Caenorhabditis elegans] 8.0E-73
blast CAA94774 Hypothetical protein F58E6.4 [Caenorhabditis elegans] ref|NP_505514.1| F58E6.4 [Caenorhabditis elegans] 2.0E-65
blast CAE70269 Hypothetical protein CBG16777 [Caenorhabditis briggsae] 9.0E-13
blast CAE70269 Hypothetical protein CBG16777 [Caenorhabditis briggsae] 1.0E-12
blast CAE65273 Hypothetical protein CBG10169 [Caenorhabditis briggsae] 2.0E-12
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505514.1 1 15-37
F58E6.4 1 15-37
SNAP00000040366 1 15-37
GENEFINDER00000040371 1 4-26
Sequence
Target Sequence 
>C. ELEGANS:177825_AT
atgcaacctattttcatttcaatacttcttggcatttgcttatcaccgatatacacattg
gagtgttattattacaatgaacgttctggtggaatttctacaacccacggtaacgccttc
tgtaccgctgtcttcgatcttgacgtggaagtagctagttttggaggagtgagccattct
cgagctgcaaagagcaaatccaattggagtttttcggaggggcaagattgtcaggtggat
gacacaaatgataatcaattctatttttgtgtttgctttgaaaataactgcaacttcccc
ctcagcattactgagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCAACCTATTTTCATTTCAATAC 239 43 52 Antisense
ACTTCTTGGCATTTGCTTATCACCG 606 103 75 Antisense
TTATCACCGATATACACATTGGAGT 149 679 91 Antisense
TGGTGGAATTTCTACAACCCACGGT 192 551 138 Antisense
GACGTGGAAGTAGCTAGTTTTGGAG 534 279 193 Antisense
TGGAGGAGTGAGCCATTCTCGAGCT 284 547 213 Antisense
GAGCCATTCTCGAGCTGCAAAGAGC 483 385 222 Antisense
GGAGTTTTTCGGAGGGGCAAGATTG 319 517 257 Antisense
ATTCTATTTTTGTGTTTGCTTTGAA 611 9 309 Antisense
TTGAAAATAACTGCAACTTCCCCCT 439 703 329 Antisense
ACTTCCCCCTCAGCATTACTGAGTT 133 103 344 Antisense
Sequence Source Affymetrix Proprietary Database