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[177823_at]

Description F18H3.1 /REP_DB=WormBase Gene ID /WP=CE02191 /TR=Q19577 /GB=CAA90443.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 13      4grp_highest_8_8 > 39      4grp_high_8_8 > 04      6grp_highest_nonorm > 60      6grp_total > 14      4grp_total > 04      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 607
1 2 3 4 range
A 458.7
(-1.3)
P 		338.1
(-2.6)
P 		298.6
(-3)
P 		877.5
(3)
P 		579
B 485.5
(-0.5)
P 		358.9
(-2.5)
P 		329.1
(-3)
P 		697.5
(3)
P 		368
C 518
(-1)
P 		328.9
(-3)
P 		332.3
(-3)
P 		906
(3)
P 		577
avr 487.4
(-1) 		342
(-2.7) 		320
(-3) 		827
(3) 		507

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 283
1 2 3 4 5 6 range
A 451.2
(3)
P 		436.7
(2.6)
P 		378.3
(0.7)
P 		308.7
(-1.4)
P 		295.2
(-1.8)
P 		257.2
(-3)
P 		194
B 441.2
(1)
P 		536
(3)
P 		433.4
(0.8)
P 		364.3
(-0.6)
P 		342.3
(-1.1)
P 		252.6
(-3)
P 		283
C 484.4
(3)
P 		416.4
(1.1)
P 		345.8
(-0.9)
P 		421.8
(1.2)
P 		270.1
(-3)
P 		363.1
(-0.4)
P 		214
avr 458.9
(2.9) 		463
(3) 		385.8
(0.3) 		364.9
(-0.4) 		302.5
(-2.6) 		291
(-3) 		172
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 167
(3)
P 		73.4
(-3)
P 		78.1
(-2.7)
P 		128.4
(0.5)
P 		94
B 154.8
(3)
P 		88.6
(-2.1)
P 		76.3
(-3)
P 		148.6
(2.5)
P 		79
C 171.5
(3)
P 		94.5
(-3)
P 		115.8
(-1.3)
P 		152.8
(1.5)
P 		77
avr 164.4
(3) 		85.5
(-3) 		90.1
(-2.7) 		143.3
(1.4) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 117
1 2 3 4 5 6 range
A 83.2
(-1.7)
P 		155.3
(3)
P 		82.4
(-1.7)
P 		78.3
(-2)
P 		89.1
(-1.3)
P 		62.6
(-3)
P 		93
B 153.5
(1.2)
P 		178.4
(3)
P 		105.2
(-2.2)
P 		96.5
(-2.8)
P 		139.1
(0.2)
P 		94.2
(-3)
P 		84
C 179.5
(3)
P 		127
(-0.3)
P 		88
(-2.8)
P 		84.8
(-3)
P 		87.4
(-2.8)
P 		121.5
(-0.7)
P 		95
avr 138.7
(1.7) 		153.6
(3) 		91.9
(-2.5) 		86.5
(-3) 		105.2
(-1.3) 		92.8
(-2.4) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177823_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17596
Sequence Type Exemplar sequence
Representative Public ID F18H3.1 NCBI
Target Description F18H3.1 /REP_DB=WormBase Gene ID /WP=CE02191 /TR=Q19577 /GB=CAA90443.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077857(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077857 NCBI Caenorhabditis elegans F18H3.1 (F18H3.1) mRNA, complete cds. 11/11 None
SNAP00000001957 ENSEMBL cdna:SNAP chromosome:CEL140:X:13519582:13521319:1 11/11 None
GENEFINDER00000001961 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:13519582:13521319:1 11/11 None
F18H3.1 ENSEMBL cdna:known chromosome:CEL140:X:13519582:13521319:1 gene:F18H3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13519323-13521319(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F18H3.1
Entrez Gene 184659 Entrez gene
SwissProt Q19577 EMBL-EBI
WormBase CE37505 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1639731_AT dm
DROSGENOME1:150112_AT dm
CHICKEN:GGAAFFX.13130.1.S1_AT similar to RIKEN cDNA 6330409N04 gga
CHICKEN:GGAAFFX.13130.1.S1_S_AT similar to RIKEN cDNA 6330409N04 gga
HG-U133A:214668_AT chromosome 13 open reading frame 1 hs
HG-U133A_2:214668_AT chromosome 13 open reading frame 1 hs
HG-U133_PLUS_2:214668_AT chromosome 13 open reading frame 1 hs
HU35KSUBA:H79230_AT chromosome 13 open reading frame 1 hs
HU35KSUBC:RC_N30354_AT chromosome 13 open reading frame 1 hs
HU35KSUBC:RC_AA398567_AT chromosome 13 open reading frame 1 hs
HU35KSUBD:RC_H79138_S_AT chromosome 13 open reading frame 1 hs
U133_X3P:HS.44235.1.S1_3P_AT chromosome 13 open reading frame 1 hs
U133_X3P:HS.44235.0.A1_3P_AT chromosome 13 open reading frame 1 hs
HG-U133_PLUS_2:230151_AT chromosome 13 open reading frame 1 hs
HG-U133B:230151_AT chromosome 13 open reading frame 1 hs
HG-U95AV2:36017_AT chromosome 13 open reading frame 1 hs
HG-U95B:47945_AT chromosome 13 open reading frame 1 hs
HG-U95E:71462_AT chromosome 13 open reading frame 1 hs
HG-U95E:71465_G_AT chromosome 13 open reading frame 1 hs
MG-U74BV2:107461_AT RIKEN cDNA 6330409N04 gene mm
MOE430A:1429723_AT RIKEN cDNA 6330409N04 gene mm
MOUSE430A_2:1429723_AT RIKEN cDNA 6330409N04 gene mm
MOUSE430_2:1429723_AT RIKEN cDNA 6330409N04 gene mm
MG-U74AV2:161055_R_AT RIKEN cDNA 6330409N04 gene, mRNA (cDNA clone MGC:47174 IMAGE:5344409) mm
MU19KSUBC:TC35218_AT RIKEN cDNA 6330409N04 gene, mRNA (cDNA clone MGC:47174 IMAGE:5344409) mm
RG-U34B:RC_AA848948_AT similar to RIKEN cDNA 6330409N04 (predicted) rn
RAE230A:1374515_AT similar to RIKEN cDNA 6330409N04 (predicted) rn
RAT230_2:1374515_AT similar to RIKEN cDNA 6330409N04 (predicted) rn
RG-U34C:RC_AI105451_AT Similar to RIKEN cDNA 6330409N04 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAA90443 Hypothetical protein F18H3.1 [Caenorhabditis elegans] ref|NP_510258.2| F18H3.1 [Caenorhabditis elegans] 1.0E-116
blast CAE63133 Hypothetical protein CBG07432 [Caenorhabditis briggsae] 3.0E-56
blast XP_782386 PREDICTED: similar to CLLL6 protein [Strongylocentrotus purpuratus] 2.0E-42
Protein Domains
Database ID Description E-Value
Pfam IPR003877 EMBL-EBI SPla/RYanodine receptor SPRY 9.5E-25
Sequence
Target Sequence 
>C. ELEGANS:177823_AT
atatccgtggttggttttcatgtttggcaccgtgcttcgactgtgtggaaggctccagtt
ttgacaatgcctcgtatccacgatttaacgcagtctaccagccaacagtacgactagatg
tggggagaatgggtaaaaatgtagttattctcaaagaaggagaacgaatttgcggtattg
gaggagcgattgcaactgttccaattgtgcaaaacaaagcatatttccaagtgacagttc
aacaaaccggaacatggggaataggcttaggacaaaagcaatcaccattcgacaaagtcc
catgcactgaaaagttttggggaatcagagataatggagacattgcaaaggaaaatgagg
tcgtggcaaaaatgacaaaaactgtggaggaaggagatgttgtgggtgttgcatatgacc
acgtggagctcaagttctatgtaaacggaaaagcagtggaagatgtgataactggagttc
gaggcccggtttaccctatggtgtatgttgacgactcagcaattctcgatctcaaattca
aaaacttcacggaagctccaccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATATCCGTGGTTGGTTTTCATGTTT 153 21 86 Antisense
CATGTTTGGCACCGTGCTTCGACTG 95 209 104 Antisense
AGTTTTGACAATGCCTCGTATCCAC 315 63 142 Antisense
ATCCACGATTTAACGCAGTCTACCA 5 41 161 Antisense
GTCTACCAGCCAACAGTACGACTAG 681 467 178 Antisense
GGAGGAGCGATTGCAACTGTTCCAA 652 515 265 Antisense
GCAATCACCATTCGACAAAGTCCCA 194 321 363 Antisense
GGTGTTGCATATGACCACGTGGAGC 84 501 490 Antisense
GCCCGGTTTACCCTATGGTGTATGT 114 287 569 Antisense
GACGACTCAGCAATTCTCGATCTCA 655 379 595 Antisense
AAAAACTTCACGGAAGCTCCACCAG 307 129 625 Antisense
Sequence Source Affymetrix Proprietary Database