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[177822_s_at]

Description F16D3.2 /REP_DB=WormBase Gene ID /WP=CE17681 /TR=Q19491 /GB=CAB01499.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 48      4grp_highest_8_8 > 50      4grp_high_8_8 > 33      6grp_highest_nonorm > 83      6grp_total > 37      4grp_total > 47      6grp_highest_sub > Cu      6grp_cgc > 16      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 300
1 2 3 4 range
A 675.9
(3)
P 		449
(-1.7)
P 		386.5
(-2.9)
P 		383.3
(-3)
P 		293
B 668.9
(3)
P 		442.6
(-1.6)
P 		403.1
(-2.4)
P 		376.1
(-3)
P 		293
C 660.3
(3)
P 		420.8
(-3)
P 		441.7
(-2.5)
P 		458.4
(-2.1)
P 		240
avr 668.4
(3) 		437.5
(-2.3) 		410.4
(-2.9) 		405.9
(-3) 		263

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 296
1 2 3 4 5 6 range
A 563.5
(2.9)
P 		546.6
(2.5)
P 		369.2
(-1.3)
P 		477.1
(1)
P 		292.9
(-3)
P 		569
(3)
P 		276
B 513.4
(1.4)
P 		526.7
(1.7)
P 		337.2
(-3)
P 		420.1
(-0.9)
P 		528.1
(1.7)
P 		579.7
(3)
P 		243
C 588.9
(3)
P 		537.9
(1.1)
P 		491.2
(-0.5)
P 		423.7
(-3)
P 		486
(-0.7)
P 		472
(-1.2)
P 		165
avr 555.3
(3) 		537.1
(2.3) 		399.2
(-3) 		440.3
(-1.4) 		435.7
(-1.6) 		540.2
(2.4) 		156
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 246.1
(3)
P 		97.5
(-1.7)
P 		101
(-1.6)
P 		56.1
(-3)
P 		190
B 213.3
(3)
P 		109.3
(-1.7)
P 		93.4
(-2.4)
P 		80.1
(-3)
P 		133
C 218.6
(3)
P 		121
(-1.1)
P 		153.9
(0.3)
P 		77.3
(-3)
P 		141
avr 226
(3) 		109.3
(-1.5) 		116.1
(-1.3) 		71.2
(-3) 		155

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 138
1 2 3 4 5 6 range
A 103.9
(-1.8)
P 		194.4
(3)
P 		80.4
(-3)
P 		121
(-0.9)
P 		88.4
(-2.6)
P 		138.5
(0.1)
P 		114
B 178.7
(1.3)
P 		175.3
(1.2)
P 		81.9
(-3)
P 		111.3
(-1.7)
P 		214.6
(2.9)
P 		216.2
(3)
P 		134
C 218.3
(3)
P 		164.1
(0.6)
P 		125
(-1.2)
P 		85.2
(-3)
P 		157.2
(0.2)
P 		158
(0.3)
P 		133
avr 167
(2.2) 		177.9
(3) 		95.8
(-3) 		105.8
(-2.3) 		153.4
(1.2) 		170.9
(2.5) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177822_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.854
Sequence Type Exemplar sequence
Representative Public ID F16D3.2 NCBI
Target Description F16D3.2 /REP_DB=WormBase Gene ID /WP=CE17681 /TR=Q19491 /GB=CAB01499.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059868(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059868 NCBI Caenorhabditis elegans RNAi Spreading Defective (see also sid) family member (rsd-6) (rsd-6) mRNA, complete cds. 9/11 None
F16D3.2.2 ENSEMBL cdna:known chromosome:CEL140:I:8365941:8371980:-1 gene:F16D3.2 9/11 None
F16D3.2.1 ENSEMBL cdna:known chromosome:CEL140:I:8366379:8371980:-1 gene:F16D3.2 9/11 None
F16D3.2.3 ENSEMBL cdna:known chromosome:CEL140:I:8366379:8371983:-1 gene:F16D3.2 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000036926 6/11 Cross Hyb Matching Probes None
GENEFINDER00000036935 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8366531-8371897(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rsd-6
Ensembl F16D3.2
Entrez Gene 172621 Entrez gene
SwissProt Q19491 EMBL-EBI
WormBase CE32384 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01499 Hypothetical protein F16D3.2 [Caenorhabditis elegans] ref|NP_492269.2| RNAi Spreading Defective (see also sid) family member (rsd-6) [Caenorhabditis elegans] 0.0
blast CAE60463 Hypothetical protein CBG04074 [Caenorhabditis briggsae] 1.0E-45
Protein Domains
Database ID Description E-Value
Pfam IPR008191 EMBL-EBI Maternal tudor protein 2.9E-25
Sequence
Target Sequence 
>C. ELEGANS:177822_S_AT
gagacgcagctcatttttctgttcattctggacagtggattccgaaaagttgtaccaagt
tccgatttgagattaatgccgcaaaagtttgcaaaactaccaccatttgcaattccttgc
actctcgatgaatcagaggatgacaaggataatattgaatttggtggaaaggaatattta
tccaatggtcctggaaatctcagtgttacttgcagcggagccgcaacagcctctgtaagg
gtttttaatcatttaggacgggatcctcacatatccaataaagttattctctacatgcca
cggtggacccttgacgaatatcaaagacgtctttatctgaacttcaaatcatgtttctat
ctaaatgttgtcgtagaaacggaccgaaacactcatttcacaaatttctttacttttttg
ccgaaacgatgtttcaagacttcgagattttttttgttttatgcccgttttctactgttg
tggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGACGCAGCTCATTTTTCTGTTCA 126 395 1765 Antisense
AAACTACCACCATTTGCAATTCCTT 37 125 1858 Antisense
CAATTCCTTGCACTCTCGATGAATC 19 181 1874 Antisense
TCAGTGTTACTTGCAGCGGAGCCGC 428 623 1964 Antisense
AGCCGCAACAGCCTCTGTAAGGGTT 675 81 1983 Antisense
TAGGACGGGATCCTCACATATCCAA 211 653 2018 Antisense
TAAAGTTATTCTCTACATGCCACGG 293 641 2043 Antisense
CCACGGTGGACCCTTGACGAATATC 24 265 2062 Antisense
GACGTCTTTATCTGAACTTCAAATC 99 377 2090 Antisense
AACGGACCGAAACACTCATTTCACA 451 143 2142 Antisense
TATGCCCGTTTTCTACTGTTGTGGT 254 653 2224 Antisense
Sequence Source Affymetrix Proprietary Database