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[177821_at]

Description F44E5.3 /REP_DB=WormBase Gene ID /WP=CE18678 /TR=Q9XU95 /GB=CAB05511.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 76      4grp_total > 10      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 1.8
(-3)
A 		3.1
(-1.4)
A 		2.4
(-2.3)
A 		6.8
(3)
A 		5
B 2.2
(-3)
A 		4.7
(0.9)
A 		2.9
(-1.9)
A 		6
(3)
A 		4
C 3.6
(-2.9)
A 		3.7
(-2.9)
A 		2.9
(-3)
A 		63.6
(3)
A 		61
avr 2.5
(-3) 		3.8
(-2.7) 		2.7
(-2.9) 		25.5
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 2.6
(-2.7)
A 		0.9
(-3)
A 		3.7
(-2.5)
A 		5.8
(-2.1)
A 		2.8
(-2.6)
A 		32.9
(3)
A 		32
B 2
(-2.7)
A 		1.9
(-2.8)
A 		3.9
(-0.7)
A 		7.5
(3)
A 		1.7
(-3)
A 		4.9
(0.3)
A 		6
C 2.5
(-3)
A 		6.2
(-1.9)
A 		2.8
(-2.9)
A 		20.1
(2.4)
A 		3.4
(-2.7)
A 		21.9
(3)
A 		19
avr 2.4
(-3) 		3
(-2.8) 		3.5
(-2.6) 		11.1
(-0) 		2.6
(-2.9) 		19.9
(3) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 0.6
(-3)
A 		0.7
(-1.5)
A 		0.6
(-3)
A 		1
(3)
A 		0
B 0.7
(-3)
A 		1.2
(2)
A 		0.7
(-3)
A 		1.3
(3)
A 		1
C 1.2
(-2.9)
A 		1.1
(-2.9)
A 		1
(-3)
A 		10.7
(3)
A 		10
avr 0.8
(-3) 		1
(-2.7) 		0.8
(-3) 		4.3
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 0.5
(-2.8)
A 		0.3
(-3)
A 		0.8
(-2.6)
A 		1.5
(-2.1)
A 		0.8
(-2.6)
A 		8
(3)
A 		8
B 0.7
(-2.6)
A 		0.6
(-3)
A 		0.9
(-1.7)
A 		2
(3)
A 		0.7
(-2.6)
A 		1.8
(2.1)
A 		1
C 0.9
(-2.8)
A 		1.9
(-1.9)
A 		0.7
(-3)
A 		4
(0)
A 		1.1
(-2.6)
A 		7.3
(3)
A 		7
avr 0.7
(-3) 		0.9
(-2.8) 		0.8
(-2.9) 		2.5
(-0.8) 		0.9
(-2.8) 		5.7
(3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177821_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3864
Sequence Type Exemplar sequence
Representative Public ID F44E5.3 NCBI
Target Description F44E5.3 /REP_DB=WormBase Gene ID /WP=CE18678 /TR=Q9XU95 /GB=CAB05511.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064110(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064110 NCBI Caenorhabditis elegans F44E5.3 (F44E5.3) mRNA, complete cds. 11/11 None
GENEFINDER00000031922 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11762071:11765567:-1 11/11 None
F44E5.3 ENSEMBL cdna:known chromosome:CEL140:II:11762071:11765567:-1 gene:F44E5.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000031912 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11762067-11765564(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F44E5.3
Entrez Gene 185736 Entrez gene
SwissProt Q9XU95 EMBL-EBI
WormBase CE18678 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05511 Hypothetical protein F44E5.3 [Caenorhabditis elegans] ref|NP_496511.1| F44E5.3 [Caenorhabditis elegans] 0.0
blast AAK20066 Hypothetical protein F47D12.5 [Caenorhabditis elegans] ref|NP_498378.1| F47D12.5 [Caenorhabditis elegans] sp|Q09562|YR45_CAEEL Hypothetical protein F47D12.5 in chromosome III 2.0E-26
blast CAB05510 Hypothetical protein F44E5.2 [Caenorhabditis elegans] ref|NP_496512.1| F44E5.2 [Caenorhabditis elegans] 8.0E-26
Sequence
Target Sequence 
>C. ELEGANS:177821_AT
cgtactacactcactcatgacatgcttatcgaactttgtaagaaacatccaaacattgaa
caaattggagtgattgttaatgcactacaattggaaattctgaaaacaatggaagaagtt
atgatgaatagtgaaggctatatcttctatcttcaaaacagcattttctctccaaatttt
aatgatttattaaaactgattgctgaaaagaaaatagaaattcgaagatcaatggtgtct
ttgctagtatcttgtttcctactttatatggattttattaaacacgaattaacagaagac
gcatcacaattcattaaccacattccgcaagttaataaagatttagcaatgaaagcatac
cacaaagaagtgctgatagtcaacatttgtcaagctattgaagactatgattccacgact
gattgcgatgaatataaggtcctcaaaaaaacactgctgagttgtgagatggatgaggca
agagactgtatcaaatggacactggataaaggagctatgccgacgtttactattg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTACTACACTCACTCATGACATGC 460 247 802 Antisense
CTCATGACATGCTTATCGAACTTTG 97 229 815 Antisense
AGGCTATATCTTCTATCTTCAAAAC 665 51 936 Antisense
TCAAAACAGCATTTTCTCTCCAAAT 663 629 954 Antisense
GTGTCTTTGCTAGTATCTTGTTTCC 149 487 1036 Antisense
GTATCTTGTTTCCTACTTTATATGG 518 453 1048 Antisense
GACGCATCACAATTCATTAACCACA 243 381 1099 Antisense
AATTCATTAACCACATTCCGCAAGT 293 177 1109 Antisense
GACTATGATTCCACGACTGATTGCG 536 371 1204 Antisense
TAAGGTCCTCAAAAAAACACTGCTG 458 637 1236 Antisense
GGAGCTATGCCGACGTTTACTATTG 339 521 1312 Antisense
Sequence Source Affymetrix Proprietary Database