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[177816_at]

Description F49H6.3 /REP_DB=WormBase Gene ID /WP=CE20830 /TR=Q9XUZ7 /GB=CAB04447.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 47      4grp_total > 21      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 50.8
(-1.7)
P 		77.9
(2.1)
A 		42
(-3)
A 		84.2
(3)
A 		42
B 96.4
(3)
P 		59.8
(-1.4)
P 		68
(-0.4)
P 		46.3
(-3)
A 		50
C 69.6
(-0.7)
P 		52.9
(-2.4)
A 		46.5
(-3)
P 		105.8
(3)
A 		59
avr 72.3
(1.5) 		63.5
(-0.5) 		52.2
(-3) 		78.8
(3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 104.6
(3)
P 		65.1
(-3)
P 		65.7
(-2.9)
A 		102.9
(2.7)
P 		70.6
(-2.2)
A 		81.9
(-0.4)
P 		40
B 86.9
(0)
P 		80.5
(-0.4)
M 		67.2
(-1.1)
A 		36
(-3)
A 		43.3
(-2.6)
A 		136.9
(3)
P 		101
C 83.9
(1.1)
P 		78.9
(0.5)
P 		53.1
(-2.4)
A 		51.2
(-2.6)
M 		47.7
(-3)
P 		100.6
(3)
P 		53
avr 91.8
(1.3) 		74.8
(-0.6) 		62
(-2.1) 		63.4
(-1.9) 		53.9
(-3) 		106.5
(3) 		53
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 18.5
(3)
P 		16.9
(1.7)
A 		11
(-3)
A 		12.3
(-2)
A 		8
B 30.7
(3)
P 		14.8
(-1.6)
P 		15.7
(-1.3)
P 		9.9
(-3)
A 		21
C 23
(3)
P 		15.2
(-3)
A 		16.2
(-2.2)
P 		17.8
(-1)
A 		8
avr 24.1
(3) 		15.6
(-1.7) 		14.3
(-2.4) 		13.3
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 19.3
(-0.5)
P 		23.2
(1.5)
P 		14.3
(-3)
A 		26.1
(3)
P 		21.3
(0.6)
A 		19.9
(-0.2)
P 		12
B 30.3
(0)
P 		26.8
(-0.5)
M 		16.3
(-2)
A 		9.5
(-3)
A 		17.6
(-1.8)
A 		51.1
(3)
P 		42
C 31.1
(2.3)
P 		24.1
(0.5)
P 		13.5
(-2.2)
A 		10.3
(-3)
M 		15.4
(-1.7)
P 		33.7
(3)
P 		23
avr 26.9
(0.6) 		24.7
(-0) 		14.7
(-3) 		15.3
(-2.8) 		18.1
(-2) 		34.9
(3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177816_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12675
Sequence Type Exemplar sequence
Representative Public ID F49H6.3 NCBI
Target Description F49H6.3 /REP_DB=WormBase Gene ID /WP=CE20830 /TR=Q9XUZ7 /GB=CAB04447.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074906(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074906 NCBI Caenorhabditis elegans F49H6.3 (F49H6.3) mRNA, complete cds. 11/11 None
SNAP00000000003 ENSEMBL cdna:SNAP chromosome:CEL140:V:17016986:17017904:1 10/11 None
GENEFINDER00000000016 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17016986:17022378:1 11/11 None
F49H6.3 ENSEMBL cdna:known chromosome:CEL140:V:17016986:17018279:1 gene:F49H6.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075006 1/11 Cross Hyb Matching Probes A 
SNAP00000030297 1/11 Cross Hyb Matching Probes None
GENEFINDER00000030304 1/11 Cross Hyb Matching Probes None
T27C5.8 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17016985-17018279(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49H6.3
Entrez Gene 186071 Entrez gene
SwissProt Q9XUZ7 EMBL-EBI
WormBase CE20830 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04439 Hypothetical protein F49H6.4 [Caenorhabditis elegans] ref|NP_507308.1| Serpentine Receptor, class H family member (srh-97) [Caenorhabditis elegans] 0.0
blast CAC35812 Hypothetical protein F21H7.11 [Caenorhabditis elegans] ref|NP_507100.2| Serpentine Receptor, class H family member (srh-95) [Caenorhabditis elegans] 1.0E-131
blast CAB04447 Hypothetical protein F49H6.3 [Caenorhabditis elegans] ref|NP_507307.1| F49H6.3 [Caenorhabditis elegans] 2.0E-98
blast CAB04447 Hypothetical protein F49H6.3 [Caenorhabditis elegans] ref|NP_507307.1| F49H6.3 [Caenorhabditis elegans] 2.0E-89
blast CAB04447 Hypothetical protein F49H6.3 [Caenorhabditis elegans] ref|NP_507307.1| F49H6.3 [Caenorhabditis elegans] 4.0E-89
blast CAB04869 Hypothetical protein T27C5.8 [Caenorhabditis elegans] ref|NP_507407.1| T27C5.8 [Caenorhabditis elegans] 5.0E-77
blast CAA20971 Hypothetical protein F49H6.13 [Caenorhabditis elegans] emb|CAB04448.1| Hypothetical protein F49H6.13 [Caenorhabditis elegans] ref|NP_507304.1| F49H6.13 [Caenorhabditis elegans] 5.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR009545 EMBL-EBI Protein of unknown function DUF1164 2.2E-122
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507307.1 4 7-29,49-71,91-113,128-150
F49H6.3 4 7-29,49-71,91-113,128-150
SNAP00000000003 4 7-29,49-71,91-113,128-150
GENEFINDER00000000016 10 7-29,49-71,91-113,128-150,374-391,395-417,438-460,506-528,548-570,580-602
Sequence
Target Sequence 
>C. ELEGANS:177816_AT
caagcgtctgttctttactgtggtcaccattgccgcaatggcctgcaacttttttggggt
cctctcgaatgcttgggtgacagattggagtggaagcatcggactttttccactttgggt
tgtcaccattggatggtatgtcgctgcaagtttggtgatggaagtctccctcttttgtac
cgtcttagtcctcattcagctgatagtcacatcaaataacatcagaaggaatggttactc
tcctacgcagagaggaaaatttatttcaattgctgtcctgtcactgttcatcactatttt
aacagttgttggcgttactttaattggagtcaatttgccgcatatgaacaggaagtataa
tgataatgctacacttggatactctgcatggatcagtgtagccggtgctgtgttctacct
cgttgtcgctggattatcgaatagctacgcacgtgtagagtgcggttcttctaagttgca
cgataa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGCGTCTGTTCTTTACTGTGGTC 319 185 24 Antisense
TTACTGTGGTCACCATTGCCGCAAT 656 681 38 Antisense
CTTTTTTGGGGTCCTCTCGAATGCT 680 221 72 Antisense
GGTCCTCTCGAATGCTTGGGTGACA 319 501 81 Antisense
GAAGGAATGGTTACTCTCCTACGCA 451 333 248 Antisense
GGAGTCAATTTGCCGCATATGAACA 455 517 349 Antisense
ATAATGCTACACTTGGATACTCTGC 5 27 386 Antisense
CAGTGTAGCCGGTGCTGTGTTCTAC 510 205 417 Antisense
CCTCGTTGTCGCTGGATTATCGAAT 481 267 441 Antisense
GATTATCGAATAGCTACGCACGTGT 278 429 455 Antisense
GCGGTTCTTCTAAGTTGCACGATAA 148 293 485 Antisense
Sequence Source Affymetrix Proprietary Database