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[177809_s_at]

Description F25H5.5 /REP_DB=WormBase Gene ID /WP=CE15901 /TR=O17838 /GB=CAB02986.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 68      4grp_highest_8_8 > 45      4grp_high_8_8 > 10      6grp_highest_nonorm > 82      6grp_total > 69      4grp_total > 22      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 212
1 2 3 4 range
A 383.5
(3)
P 		233.5
(-1.4)
P 		178.6
(-3)
P 		343.8
(1.8)
P 		205
B 310.1
(-1.3)
P 		276.9
(-3)
P 		306.4
(-1.4)
P 		390.9
(3)
P 		114
C 378.5
(3)
P 		244.8
(-3)
P 		277
(-1.6)
P 		335.2
(1.1)
P 		134
avr 357.4
(3) 		251.7
(-3) 		254
(-2.9) 		356.6
(3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 248
1 2 3 4 5 6 range
A 360.9
(0.5)
P 		226.9
(-3)
P 		317.8
(-0.7)
P 		284.1
(-1.5)
P 		459.5
(3)
P 		305.7
(-1)
P 		233
B 358.5
(1)
P 		323
(-0.5)
P 		261.9
(-3)
P 		318.7
(-0.7)
P 		278.3
(-2.3)
P 		407
(3)
P 		145
C 232.7
(-2.3)
P 		348.5
(1.3)
P 		280.2
(-0.8)
P 		227.6
(-2.5)
P 		211.8
(-3)
P 		401.4
(3)
P 		190
avr 317.4
(-0.4) 		299.5
(-1.6) 		286.6
(-2.4) 		276.8
(-3) 		316.5
(-0.5) 		371.4
(3) 		95
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 139.6
(3)
P 		50.7
(-2.7)
P 		46.7
(-3)
P 		50.3
(-2.8)
P 		93
B 98.9
(3)
P 		68.4
(-3)
P 		71
(-2.5)
P 		83.3
(-0.1)
P 		31
C 125.3
(3)
P 		70.4
(-1.8)
P 		96.5
(0.5)
P 		56.5
(-3)
P 		69
avr 121.3
(3) 		63.2
(-3) 		71.4
(-2.2) 		63.4
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 66.5
(-3)
P 		80.7
(-1.8)
P 		69.2
(-2.8)
P 		72.1
(-2.5)
P 		138.7
(3)
P 		74.4
(-2.3)
P 		72
B 124.7
(1.2)
P 		107.5
(-0)
P 		63.6
(-3)
P 		84.4
(-1.6)
P 		113.1
(0.4)
P 		151.8
(3)
P 		88
C 86.3
(-0.3)
P 		106.3
(1.1)
P 		71.3
(-1.3)
P 		45.8
(-3)
P 		68.5
(-1.5)
P 		134.4
(3)
P 		89
avr 92.5
(-0.1) 		98.2
(0.5) 		68
(-2.9) 		67.4
(-3) 		106.8
(1.5) 		120.2
(3) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177809_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.495
Sequence Type Exemplar sequence
Representative Public ID F25H5.5 NCBI
Target Description F25H5.5 /REP_DB=WormBase Gene ID /WP=CE15901 /TR=O17838 /GB=CAB02986.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060055(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060055 NCBI Caenorhabditis elegans F25H5.5 (F25H5.5) mRNA, complete cds. 11/11 None
SNAP00000024318 ENSEMBL cdna:SNAP chromosome:CEL140:I:9168210:9171385:-1 11/11 None
GENEFINDER00000024328 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9168210:9171385:-1 11/11 None
F25H5.5 ENSEMBL cdna:known chromosome:CEL140:I:9168104:9171349:-1 gene:F25H5.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9168140-9171235(-) 94.72 100.0
Public Domain and Genome References
Gene Title
Ensembl F25H5.5
Entrez Gene 172742 Entrez gene
WormBase CE15901 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.6235.1.S1_S_AT similar to claspin cfa
DROSOPHILA_2:1633138_AT dm
CHICKEN:GGA.11364.1.S1_S_AT claspin homolog (Xenopus laevis) gga
CHICKEN:GGAAFFX.1568.1.S1_AT claspin homolog (Xenopus laevis) gga
CHICKEN:GGAAFFX.25465.1.S1_S_AT claspin homolog (Xenopus laevis) gga
HG-U133_PLUS_2:219621_AT claspin homolog (Xenopus laevis) hs
HG-U133A:219621_AT claspin homolog (Xenopus laevis) hs
HG-FOCUS:219621_AT claspin homolog (Xenopus laevis) hs
HG-U133A_2:219621_AT claspin homolog (Xenopus laevis) hs
HU35KSUBA:HUM_ALU_AT claspin homolog (Xenopus laevis) hs
HU35KSUBB:HUM_ALU_AT claspin homolog (Xenopus laevis) hs
HU35KSUBC:HUM_ALU_AT claspin homolog (Xenopus laevis) hs
HU35KSUBD:HUM_ALU_AT claspin homolog (Xenopus laevis) hs
HUGENEFL:HUM_ALU_AT claspin homolog (Xenopus laevis) hs
HC-G110:HUM_ALU_AT claspin homolog (Xenopus laevis) hs
U133_X3P:G11545824_3P_AT claspin homolog (Xenopus laevis) hs
U133_X3P:HS2.175613.1.S1_3P_AT claspin homolog (Xenopus laevis) hs
HG-U133_PLUS_2:1553120_AT claspin homolog (Xenopus laevis) hs
HG-U95D:72139_AT claspin homolog (Xenopus laevis) hs
HG-U95D:92123_AT claspin homolog (Xenopus laevis) hs
MG-U74BV2:111511_AT claspin homolog (Xenopus laevis) mm
MG-U74CV2:137209_F_AT claspin homolog (Xenopus laevis) mm
MOE430B:1456280_AT claspin homolog (Xenopus laevis) mm
MOUSE430_2:1456280_AT claspin homolog (Xenopus laevis) mm
MOE430B:1441984_AT claspin homolog (Xenopus laevis) mm
MOUSE430_2:1441984_AT claspin homolog (Xenopus laevis) mm
MU19KSUBB:TC31315_AT claspin homolog (Xenopus laevis) mm
RAE230B:1391077_AT Claspin homolog (Xenopus laevis) (predicted) rn
RAT230_2:1391077_AT Claspin homolog (Xenopus laevis) (predicted) rn
RG-U34B:RC_AA851257_AT Claspin homolog (Xenopus laevis) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAB02986 Hypothetical protein F25H5.5 [Caenorhabditis elegans] ref|NP_492456.1| F25H5.5 [Caenorhabditis elegans] 0.0
blast CAE70383 Hypothetical protein CBG16944 [Caenorhabditis briggsae] 1.0E-135
blast CAE70383 Hypothetical protein CBG16944 [Caenorhabditis briggsae] 1.0E-130
blast CAE70383 Hypothetical protein CBG16944 [Caenorhabditis briggsae] 1.0E-125
blast AAG24516 Claspin [Xenopus laevis] sp|Q9DF50|CLSPN_XENLA Claspin (xClaspin) 5.0E-12
Sequence
Target Sequence 
>C. ELEGANS:177809_S_AT
aacttcaagaccgtctacttgccgacggagatctcggtggagttgagacgaatcgtcaat
tccgtttcaagttacgagaagaagttcaaattcaaatgggagatgatgtagatggaaatg
atggtgatggagaagatgatgaaccagaagaagatgaacaaaaaaagaaggaacgagccg
aaatgatcaagttcaaatatgaacatgcggaagaattgatgttgctcgatgagccgaaac
aaggcgatgatatgtttgctcgcgccggaaagttattcaagaaaactgaaaaaactgtaa
tcgaattctccgaagaaacaatgttcaaaccgagactcagcaagccatctctactgtcaa
aaaccactcttgctgtcacatttcaagaagttctgaatggacctggaggtgcagcaaaac
agatgtatgtgcaaagctttgagaagccaactcctctctcaactggacatata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTTCAAGACCGTCTACTTGCCGA 479 139 1610 Antisense
ACTTGCCGACGGAGATCTCGGTGGA 544 101 1626 Antisense
ATCGTCAATTCCGTTTCAAGTTACG 605 33 1661 Antisense
GATGTTGCTCGATGAGCCGAAACAA 296 409 1827 Antisense
GGCGATGATATGTTTGCTCGCGCCG 312 541 1852 Antisense
TTTGCTCGCGCCGGAAAGTTATTCA 643 647 1864 Antisense
AAAACTGTAATCGAATTCTCCGAAG 496 129 1900 Antisense
TCAGCAAGCCATCTCTACTGTCAAA 283 623 1946 Antisense
TACTGTCAAAAACCACTCTTGCTGT 473 647 1961 Antisense
AGCTTTGAGAAGCCAACTCCTCTCT 376 79 2044 Antisense
AACTCCTCTCTCAACTGGACATATA 495 141 2058 Antisense
Sequence Source Affymetrix Proprietary Database