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[177785_s_at]

Description T04D3.5 /REP_DB=WormBase Gene ID /WP=CE13161 /TR=O02299 /GB=CAB03287.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 57      6grp_highest_nonorm > 92      6grp_total > 58      4grp_total > 53      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 228
1 2 3 4 range
A 267.3
(3)
P 		148.2
(-0.1)
P 		122
(-0.8)
M 		39.6
(-3)
A 		228
B 225.6
(3)
P 		198.8
(2.1)
P 		125
(-0.5)
P 		54
(-3)
A 		172
C 239.8
(3)
P 		148.5
(0.2)
P 		109.6
(-1)
A 		43.6
(-3)
A 		196
avr 244.2
(3) 		165.2
(0.6) 		118.9
(-0.8) 		45.7
(-3) 		199

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 380
1 2 3 4 5 6 range
A 340.2
(0.6)
P 		230.1
(-1.7)
P 		211.1
(-2.1)
P 		167.6
(-3)
P 		218.5
(-2)
P 		458.5
(3)
P 		291
B 398.6
(0.6)
P 		275.9
(-1.4)
P 		311.6
(-0.9)
P 		181.1
(-3)
P 		294
(-1.2)
P 		548
(3)
P 		367
C 430
(1)
P 		275.7
(-2)
P 		225.3
(-2.9)
P 		255.5
(-2.4)
P 		221.5
(-3)
P 		535.7
(3)
P 		314
avr 389.6
(0.6) 		260.6
(-1.9) 		249.3
(-2.1) 		201.4
(-3) 		244.7
(-2.2) 		514.1
(3) 		313
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 97.3
(3)
P 		32.2
(-1.3)
P 		31.9
(-1.3)
M 		5.8
(-3)
A 		92
B 71.9
(3)
P 		49.1
(0.7)
P 		29
(-1.3)
P 		11.5
(-3)
A 		60
C 79.4
(3)
P 		42.7
(-0.1)
P 		38.2
(-0.4)
A 		7.3
(-3)
A 		72
avr 82.9
(3) 		41.3
(-0.3) 		33
(-1) 		8.2
(-3) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 62.7
(-1.2)
P 		81.8
(0.4)
P 		46
(-2.7)
P 		42.5
(-3)
P 		65.9
(-1)
P 		111.6
(3)
P 		69
B 138.7
(0.5)
P 		91.8
(-1.3)
P 		75.7
(-1.9)
P 		48
(-3)
P 		119.5
(-0.3)
P 		204.3
(3)
P 		156
C 159.4
(2.1)
P 		84.1
(-1.5)
P 		57.4
(-2.7)
P 		51.4
(-3)
P 		71.7
(-2)
P 		179.3
(3)
P 		128
avr 120.3
(0.7) 		85.9
(-1) 		59.7
(-2.4) 		47.3
(-3) 		85.7
(-1) 		165.1
(3) 		118
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177785_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1837
Sequence Type Exemplar sequence
Representative Public ID T04D3.5 NCBI
Target Description T04D3.5 /REP_DB=WormBase Gene ID /WP=CE13161 /TR=O02299 /GB=CAB03287.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060944(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060944 NCBI Caenorhabditis elegans T04D3.5 (T04D3.5) mRNA, complete cds. 11/11 A 
SNAP00000016266 ENSEMBL cdna:SNAP chromosome:CEL140:I:13337433:13338876:-1 11/11 None
GENEFINDER00000016275 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13337433:13338876:-1 11/11 None
T04D3.5 ENSEMBL cdna:known chromosome:CEL140:I:13337354:13339051:-1 gene:T04D3.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13337363-13338807(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T04D3.5
Entrez Gene 173201 Entrez gene
SwissProt O02299 EMBL-EBI
WormBase CE13161 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03287 Hypothetical protein T04D3.5 [Caenorhabditis elegans] ref|NP_493345.1| T04D3.5 [Caenorhabditis elegans] 1.0E-174
blast CAE73024 Hypothetical protein CBG20391 [Caenorhabditis briggsae] 1.0E-61
Sequence
Target Sequence 
>C. ELEGANS:177785_S_AT
gaaaactgcatctcagcaagtcgtcattagctgcaagcaactgaaacctgcgaaactcga
tatgaaaacacttcgtcaagttatggagcacgagctcgccgagctgggagacgagaagct
tcaacgaatggtgtgctcattctgctacagagctcagccaactcatcggctctacacgat
tgaagacgtaatcaagtgccaggaatgctggcgtggacccttcccatctcgtacgatttt
cgacgcacttatcactgatgtcgcggagaagtgggctgagaaggtggcgaaacgtgtcga
gtgtctgtatggaaattgggatgagaagcttacaccgatggtccgtatgcttcagaataa
tgtgctcttgtcggaggatattgcagcgttgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAACTGCATCTCAGCAAGTCGTC 1 355 309 Antisense
CAAGTCGTCATTAGCTGCAAGCAAC 670 183 325 Antisense
AGTTATGGAGCACGAGCTCGCCGAG 461 61 387 Antisense
ATGGTGTGCTCATTCTGCTACAGAG 169 51 436 Antisense
TACAGAGCTCAGCCAACTCATCGGC 8 645 454 Antisense
CCAACTCATCGGCTCTACACGATTG 580 275 466 Antisense
CTTCCCATCTCGTACGATTTTCGAC 377 219 528 Antisense
GACGCACTTATCACTGATGTCGCGG 670 379 550 Antisense
GAAACGTGTCGAGTGTCTGTATGGA 632 357 597 Antisense
CACCGATGGTCCGTATGCTTCAGAA 359 201 641 Antisense
TGTCGGAGGATATTGCAGCGTTGCT 241 553 677 Antisense
Sequence Source Affymetrix Proprietary Database