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[177774_at]

Description F31C3.2 /REP_DB=WormBase Gene ID /WP=CE17731 /TR=O62195 /GB=CAB04268.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 68      4grp_highest_8_8 > 44      4grp_high_8_8 > 08      6grp_highest_nonorm > 84      6grp_total > 59      4grp_total > 31      6grp_highest_sub > Da      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 432
1 2 3 4 range
A 760.7
(3)
P 		443.6
(-3)
P 		615.2
(0.2)
P 		614.9
(0.2)
P 		317
B 756.3
(3)
P 		429.1
(-3)
P 		430.8
(-3)
P 		559.8
(-0.6)
P 		327
C 786.6
(3)
P 		354.7
(-3)
P 		448.5
(-1.7)
P 		651.5
(1.1)
P 		432
avr 767.9
(3) 		409.1
(-3) 		498.2
(-1.5) 		608.7
(0.3) 		359

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 302
1 2 3 4 5 6 range
A 528.2
(-2.3)
P 		555.8
(-1.1)
P 		512.6
(-3)
P 		553.4
(-1.2)
P 		599.7
(0.9)
P 		646.3
(3)
P 		134
B 699
(3)
P 		539.5
(-2.3)
P 		533.2
(-2.5)
P 		519.2
(-3)
P 		567.6
(-1.4)
P 		675.5
(2.2)
P 		180
C 514.4
(-1.8)
P 		607.7
(0)
P 		456.2
(-3)
P 		611.5
(0.1)
P 		534.5
(-1.4)
P 		758.2
(3)
P 		302
avr 580.5
(-0.5) 		567.7
(-0.9) 		500.7
(-3) 		561.4
(-1.1) 		567.3
(-0.9) 		693.3
(3) 		193
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 187
1 2 3 4 range
A 277
(3)
P 		96.3
(-2.8)
P 		160.8
(-0.7)
P 		90
(-3)
P 		187
B 241.2
(3)
P 		106
(-2.7)
P 		99.8
(-3)
P 		119.3
(-2.2)
P 		141
C 260.4
(3)
P 		102
(-3)
P 		156.3
(-0.9)
P 		109.9
(-2.7)
P 		158
avr 259.5
(3) 		101.4
(-3) 		139
(-1.6) 		106.4
(-2.8) 		158

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 156
1 2 3 4 5 6 range
A 97.4
(-3)
P 		197.7
(3)
P 		111.6
(-2.2)
P 		140.4
(-0.4)
P 		181
(2)
P 		157.3
(0.6)
P 		100
B 243.2
(2.6)
P 		179.6
(-0.5)
P 		129.5
(-3)
P 		137.5
(-2.6)
P 		230.6
(2)
P 		251.9
(3)
P 		122
C 190.6
(0.2)
P 		185.4
(0)
P 		116.1
(-3)
P 		123
(-2.7)
P 		173
(-0.5)
P 		253.8
(3)
P 		138
avr 177.1
(0.4) 		187.6
(1) 		119.1
(-3) 		133.6
(-2.1) 		194.9
(1.5) 		221
(3) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177774_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.908
Sequence Type Exemplar sequence
Representative Public ID F31C3.2 NCBI
Target Description F31C3.2 /REP_DB=WormBase Gene ID /WP=CE17731 /TR=O62195 /GB=CAB04268.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026262(11), NM_001026263(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026262 NCBI Caenorhabditis elegans F31C3.2a (F31C3.2) mRNA, complete cds. 11/11 None
NM_001026263 NCBI Caenorhabditis elegans F31C3.2b (F31C3.2) mRNA, complete cds. 11/11 None
SNAP00000023484 ENSEMBL cdna:SNAP chromosome:CEL140:I:15046889:15048519:1 11/11 None
GENEFINDER00000023497 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:15046889:15048519:1 11/11 None
F31C3.2a ENSEMBL cdna:known chromosome:CEL140:I:15044491:15048605:1 gene:F31C3.2 11/11 None
F31C3.2b ENSEMBL cdna:known chromosome:CEL140:I:15044495:15048519:1 gene:F31C3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:15044425-15048450(+) 100.0 100.0
Public Domain and Genome References
Ensembl F31C3.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07197 Hypothetical protein F31C3.2a [Caenorhabditis elegans] emb|CAB04268.1| Hypothetical protein F31C3.2a [Caenorhabditis elegans] ref|NP_001021433.1| F31C3.2a [Caenorhabditis elegans] 0.0
blast CAE54895 Hypothetical protein F31C3.2b [Caenorhabditis elegans] emb|CAE54892.1| Hypothetical protein F31C3.2b [Caenorhabditis elegans] ref|NP_001021434.1| F31C3.2b [Caenorhabditis elegans] 0.0
blast CAE72551 Hypothetical protein CBG19735 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002058 EMBL-EBI PAP/25A-associated domain 1.6E-20
Pfam IPR002058 EMBL-EBI PAP/25A-associated domain 1.6E-20
Pfam IPR002058 EMBL-EBI PAP/25A-associated domain 1.6E-20
Sequence
Target Sequence 
>C. ELEGANS:177774_AT
gaagcactgggcgatggttactggaatcaataacgcacaggacggatttctgaatagtta
cacgacgattcttctcgttgtgcactatcttcaatgcggtgtcactccagcagtcattcc
gaacttgcaatacttgttcccgcacaagtttgatcgaaaacttccgcttaatgagcttct
tctgtttggcgatattgcggacaagttgcccacaagtcctcccaacacgtggtcgcttgg
agagttgctcatcggattctttcagtactataatgaattcgatttcactaatttcggatt
ttcaatccgctcgggtcaagtcattcctcgggaaaatctcccccgtgatctcatcaacag
tccgattgtcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCACTGGGCGATGGTTACTGGA 239 341 1899 Antisense
TACACGACGATTCTTCTCGTTGTGC 245 643 1957 Antisense
TCTTCAATGCGGTGTCACTCCAGCA 551 615 1986 Antisense
TCCAGCAGTCATTCCGAACTTGCAA 94 591 2004 Antisense
GAACTTGCAATACTTGTTCCCGCAC 605 349 2019 Antisense
ATGAGCTTCTTCTGTTTGGCGATAT 532 45 2069 Antisense
GCGGACAAGTTGCCCACAAGTCCTC 534 293 2095 Antisense
CACGTGGTCGCTTGGAGAGTTGCTC 382 199 2124 Antisense
GATTTTCAATCCGCTCGGGTCAAGT 236 425 2195 Antisense
TCCTCGGGAAAATCTCCCCCGTGAT 526 265 2223 Antisense
GATCTCATCAACAGTCCGATTGTCG 306 419 2245 Antisense
Sequence Source Affymetrix Proprietary Database