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[177771_at]

Description R08H2.8 /REP_DB=WormBase Gene ID /WP=CE12608 /TR=O17995 /GB=CAB04638.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 90      4grp_total > 58      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 31.9
(-0.6)
A 		51.7
(1.1)
A 		73.5
(3)
A 		3.4
(-3)
A 		70
B 45.7
(-1.5)
A 		44.1
(-3)
A 		47.2
(-0.1)
A 		50.5
(3)
A 		6
C 47.2
(3)
A 		43.8
(0.6)
A 		38.6
(-3)
A 		42.2
(-0.5)
A 		9
avr 41.6
(-0.3) 		46.5
(1.1) 		53.1
(3) 		32
(-3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 44
(0.7)
A 		55.9
(3)
A 		30.8
(-2)
A 		25.5
(-3)
A 		32.9
(-1.5)
A 		40.5
(-0)
A 		30
B 45.2
(1.6)
A 		34.4
(0.4)
A 		4.7
(-3)
A 		7.1
(-2.7)
A 		57.7
(3)
A 		33.5
(0.3)
A 		53
C 28.6
(-1.4)
A 		5.2
(-3)
A 		69.9
(1.4)
A 		93.1
(3)
P 		21.8
(-1.9)
A 		43.3
(-0.4)
A 		88
avr 39.3
(1.5) 		31.8
(-3) 		35.1
(-1) 		41.9
(3) 		37.5
(0.4) 		39.1
(1.3) 		10
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 11.6
(0.6)
A 		11.2
(0.4)
A 		19.2
(3)
A 		0.5
(-3)
A 		19
B 14.6
(3)
A 		10.9
(-2.8)
A 		10.9
(-2.8)
A 		10.8
(-3)
A 		4
C 15.6
(3)
A 		12.6
(0.9)
A 		13.5
(1.5)
A 		7.1
(-3)
A 		9
avr 13.9
(2.6) 		11.6
(0.9) 		14.5
(3) 		6.1
(-3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 8.1
(-2.3)
A 		19.9
(3)
A 		6.7
(-2.9)
A 		6.5
(-3)
A 		9.9
(-1.5)
A 		9.9
(-1.5)
A 		13
B 15.7
(0.9)
A 		11.5
(-0.2)
A 		1.1
(-3)
A 		1.9
(-2.8)
A 		23.5
(3)
A 		12.5
(0.1)
A 		22
C 10.6
(0.2)
A 		1.6
(-3)
A 		17.8
(2.7)
A 		18.7
(3)
P 		7
(-1.1)
A 		14.5
(1.5)
A 		17
avr 11.5
(0.6) 		11
(0) 		8.5
(-3) 		9
(-2.4) 		13.5
(3) 		12.3
(1.6) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177771_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13075
Sequence Type Exemplar sequence
Representative Public ID R08H2.8 NCBI
Target Description R08H2.8 /REP_DB=WormBase Gene ID /WP=CE12608 /TR=O17995 /GB=CAB04638.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074413(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074413 NCBI Caenorhabditis elegans R08H2.8 (R08H2.8) mRNA, complete cds. 11/11 None
SNAP00000012522 ENSEMBL cdna:SNAP chromosome:CEL140:V:15373453:15374830:-1 11/11 None
GENEFINDER00000012534 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15369745:15377387:-1 11/11 None
R08H2.8 ENSEMBL cdna:known chromosome:CEL140:V:15373453:15374677:-1 gene:R08H2.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15373452-15374677(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R08H2.8
Entrez Gene 187715 Entrez gene
SwissProt O17995 EMBL-EBI
WormBase CE12608 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04638 Hypothetical protein R08H2.8 [Caenorhabditis elegans] ref|NP_506814.1| R08H2.8 [Caenorhabditis elegans] 0.0
blast CAB04634 Hypothetical protein R08H2.9 [Caenorhabditis elegans] ref|NP_506813.1| R08H2.9 [Caenorhabditis elegans] 1.0E-133
blast CAB03505 Hypothetical protein ZK1037.6 [Caenorhabditis elegans] ref|NP_506799.1| ZK1037.6 [Caenorhabditis elegans] 1.0E-59
blast CAB03524 Hypothetical protein K10D11.5 [Caenorhabditis elegans] ref|NP_502490.1| K10D11.5 [Caenorhabditis elegans] 6.0E-49
blast CAE56628 Hypothetical protein CBG24384 [Caenorhabditis briggsae] 7.0E-48
Protein Domains
Database ID Description E-Value
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 1.7E-12
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.2E-21
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 4.9E-30
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 5.0E-5
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 6.3E-15
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 1.1E-14
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000012534 3 988-1010,1031-1053,1063-1085
Sequence
Target Sequence 
>C. ELEGANS:177771_AT
tagctgctacattattcactccaaattgcaacacctacttacctcaaaaattcactggtc
ccgtcaaaacatttgtgttgactgaaggaacagcattactgaacatgtcaacttattata
atgataattcaaacaccgctttaattggtagaaagggatatgttgtctccaacttctatg
ggacacctagcaatcaacaggacgcatttatggttatagacgcacgaaatgtttcatatg
atgttaaattccggattgaaattaagtttgctgatttaaatgacaccgctcaattgagca
tccagggatggaaaaatagaaaaatagtctctaatacaacctacactgcttcaaatcgtc
catttatcaatttgatctatgaagtttatggtgacagtatgggagtttattatagctcgt
atggatctactacgaaaggtcttcttatggaatatgaagttgtcaaatccactgataccg
cctctaatattttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAGCTGCTACATTATTCACTCCAAA 101 653 584 Antisense
CACTCCAAATTGCAACACCTACTTA 484 195 600 Antisense
GCAACACCTACTTACCTCAAAAATT 460 319 611 Antisense
AAATTCACTGGTCCCGTCAAAACAT 513 115 631 Antisense
GGATATGTTGTCTCCAACTTCTATG 264 511 739 Antisense
AACTTCTATGGGACACCTAGCAATC 409 139 754 Antisense
ATGACACCGCTCAATTGAGCATCCA 97 45 863 Antisense
ACAACCTACACTGCTTCAAATCGTC 88 113 919 Antisense
ATGGATCTACTACGAAAGGTCTTCT 32 51 1004 Antisense
AGTTGTCAAATCCACTGATACCGCC 400 63 1041 Antisense
ACTGATACCGCCTCTAATATTTTAT 613 97 1054 Antisense
Sequence Source Affymetrix Proprietary Database