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[177770_s_at]

Description F32H2.3 /REP_DB=WormBase Gene ID /WP=CE09878 /TR=P91870 /GB=CAB04243.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 87      4grp_highest_8_8 > 16      4grp_high_8_8 > 60      6grp_highest_nonorm > 94      6grp_total > 78      4grp_total > 59      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 314.4
(3)
P 		283.1
(-0.9)
P 		304
(1.7)
P 		266.1
(-3)
P 		48
B 275.9
(-1.4)
P 		347.4
(3)
P 		278.5
(-1.2)
P 		249.7
(-3)
P 		98
C 295.8
(0.5)
P 		286
(-0)
P 		342.9
(3)
P 		230.1
(-3)
P 		113
avr 295.4
(1.7) 		305.5
(2.7) 		308.5
(3) 		248.6
(-3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 628
1 2 3 4 5 6 range
A 478
(-1.3)
P 		314
(-2.9)
P 		306.3
(-3)
P 		364.1
(-2.4)
P 		551.3
(-0.6)
P 		923.8
(3)
P 		618
B 326.3
(-2.6)
P 		296.2
(-3)
P 		309.1
(-2.8)
P 		412.4
(-1.6)
P 		455.6
(-1.1)
P 		804.2
(3)
P 		508
C 330.7
(-3)
P 		338.6
(-2.9)
P 		390.1
(-2.4)
P 		414.6
(-2.1)
P 		533
(-0.8)
P 		894.5
(3)
P 		564
avr 378.3
(-2.3) 		316.3
(-3) 		335.2
(-2.8) 		397
(-2.1) 		513.3
(-0.9) 		874.2
(3) 		558
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 114.5
(3)
P 		61.5
(-1.2)
P 		79.5
(0.2)
P 		39
(-3)
P 		76
B 88
(3)
P 		85.8
(2.6)
P 		64.5
(-1.1)
P 		53.2
(-3)
P 		35
C 97.9
(1.4)
P 		82.2
(0.2)
P 		119.5
(3)
P 		38.8
(-3)
P 		81
avr 100.1
(3) 		76.5
(0.5) 		87.8
(1.7) 		43.7
(-3) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 233
1 2 3 4 5 6 range
A 88.1
(-2.2)
P 		111.7
(-1.3)
P 		66.7
(-3)
P 		92.4
(-2)
P 		166.4
(0.8)
P 		224.9
(3)
P 		158
B 113.5
(-2)
P 		98.6
(-2.4)
P 		75.1
(-3)
P 		109.2
(-2.1)
P 		185.1
(-0.1)
P 		299.9
(3)
P 		225
C 122.6
(-1.9)
P 		103.3
(-2.4)
P 		99.3
(-2.6)
P 		83.4
(-3)
P 		172.5
(-0.5)
P 		299.5
(3)
P 		216
avr 108.1
(-2.1) 		104.5
(-2.3) 		80.4
(-3) 		95
(-2.5) 		174.7
(-0.1) 		274.8
(3) 		194
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177770_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2320
Sequence Type Exemplar sequence
Representative Public ID F32H2.3 NCBI
Target Description F32H2.3 /REP_DB=WormBase Gene ID /WP=CE09878 /TR=P91870 /GB=CAB04243.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060013(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060013 NCBI Caenorhabditis elegans SPindle Defective family member (spd-2) (spd-2) mRNA, complete cds. 11/11 None
SNAP00000013729 ENSEMBL cdna:SNAP chromosome:CEL140:I:8975219:8978195:-1 11/11 None
GENEFINDER00000013773 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8975219:8978195:-1 11/11 None
F32H2.3.1 ENSEMBL cdna:known chromosome:CEL140:I:8975107:8978205:-1 gene:F32H2.3 11/11 None
F32H2.3.2 ENSEMBL cdna:known chromosome:CEL140:I:8975109:8978252:-1 gene:F32H2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8975149-8978126(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spd-2
Ensembl F32H2.3
Entrez Gene 172712 Entrez gene
SwissProt P91870 EMBL-EBI
WormBase CE09878 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5814 centriole inferred from direct assay QuickGO AmiGO
242 pericentriolar material inferred from direct assay QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04243 Hypothetical protein F32H2.3 [Caenorhabditis elegans] ref|NP_492414.1| SPindle Defective family member (spd-2) [Caenorhabditis elegans] gb|AAQ17186.1| SPD-2 [Caenorhabditis elegans] sp|P91870|SPD2_CAEEL Spindle-defective protein 0.0
blast Q61DP2 Spindle-defective protein 2 emb|CAE67005.1| Hypothetical protein CBG12404 [Caenorhabditis briggsae] 1.0E-161
Sequence
Target Sequence 
>C. ELEGANS:177770_S_AT
gtgttcgactcagcgatgcgacagcttccagacggatgtgtagctttctttcctgcaccc
ggtttcattgtaaagaaaaaatcggataagcgtgttgacattcgcatcgactcatcttat
attgatcttcacgatgaaaacaattttcgtacatcgtcttcgctttcgacagcctcaaca
acatcctcattccagcgacgtattcttcctggagccaaattctttgtgcatgtcgtttgg
ggtgaagaaaccatgcgaaccagacttcgattgctagaagttcgcacaggacagcatcaa
ctaatcgatggtcatgacttcacatctttccaattttccgatgaagaagttcttcgtgct
gttccagttggattccccgctatcaagcccgaggaccgtgatctgttcgctgcttcttat
cgtagtttcttcatcaattttttcacttctacaactgaattccgtgctgctacaagccgg
aagaagaaagagatatgcagcaacgacgactctactctactcgagacaacagctttccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTTCGACTCAGCGATGCGACAGC 193 485 1885 Antisense
ACGGATGTGTAGCTTTCTTTCCTGC 21 97 1916 Antisense
GCGTGTTGACATTCGCATCGACTCA 472 291 1974 Antisense
AATTTTCGTACATCGTCTTCGCTTT 146 173 2026 Antisense
TCGCTTTCGACAGCCTCAACAACAT 255 601 2044 Antisense
CCAGCGACGTATTCTTCCTGGAGCC 117 273 2076 Antisense
TGGTCATGACTTCACATCTTTCCAA 59 553 2193 Antisense
GCTGCTTCTTATCGTAGTTTCTTCA 490 295 2293 Antisense
TACAACTGAATTCCGTGCTGCTACA 567 641 2334 Antisense
ATATGCAGCAACGACGACTCTACTC 497 21 2377 Antisense
TCTACTCGAGACAACAGCTTTCCGT 89 613 2400 Antisense
Sequence Source Affymetrix Proprietary Database