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[177762_at]

Description T22H6.5 /REP_DB=WormBase Gene ID /WP=CE03700 /TR=Q22690 /GB=CAA90673.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_highest_nonorm > 40      6grp_total > 07      4grp_total > 29      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 541
1 2 3 4 range
A 869.9
(2.8)
P 		862.1
(2.7)
P 		461.4
(-3)
P 		883.1
(3)
P 		422
B 892.8
(3)
P 		789.1
(1.6)
P 		445.7
(-3)
P 		821.3
(2)
P 		447
C 829.1
(2.8)
P 		848.3
(3)
P 		352.1
(-3)
P 		790.8
(2.3)
P 		496
avr 863.9
(3) 		833.2
(2.6) 		419.7
(-3) 		831.7
(2.6) 		444

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 589
1 2 3 4 5 6 range
A 1028
(3)
P 		651.1
(-0.9)
P 		560.7
(-1.9)
P 		519.2
(-2.3)
P 		451.2
(-3)
P 		535.4
(-2.1)
P 		577
B 675.4
(3)
P 		585.1
(0.7)
P 		493.5
(-1.6)
P 		497.4
(-1.5)
P 		439
(-3)
P 		447.2
(-2.8)
P 		236
C 938
(3)
P 		526.7
(-2.5)
P 		586.7
(-1.7)
P 		559.7
(-2)
P 		486.3
(-3)
P 		577.9
(-1.8)
P 		452
avr 880.5
(3) 		587.6
(-1.2) 		547
(-1.7) 		525.4
(-2.1) 		458.8
(-3) 		520.2
(-2.1) 		422
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 213
1 2 3 4 range
A 316.7
(3)
P 		187.1
(-1)
P 		120.6
(-3)
P 		129.3
(-2.7)
P 		196
B 284.7
(3)
P 		194.9
(0)
P 		103.3
(-3)
P 		175
(-0.6)
P 		181
C 274.5
(3)
P 		243.9
(1.8)
P 		122.7
(-3)
P 		133.3
(-2.6)
P 		152
avr 292
(3) 		208.6
(0.2) 		115.5
(-3) 		145.9
(-2) 		177

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 235
1 2 3 4 5 6 range
A 189.5
(0.7)
P 		231.6
(3)
P 		122.1
(-3)
P 		131.7
(-2.5)
P 		136.1
(-2.2)
P 		130.3
(-2.6)
P 		110
B 235
(3)
P 		194.7
(0.9)
P 		119.8
(-3)
P 		131.8
(-2.4)
P 		178.4
(0.1)
P 		166.8
(-0.6)
P 		115
C 347.7
(3)
P 		160.7
(-1.8)
P 		149.3
(-2.1)
P 		112.6
(-3)
P 		157.3
(-1.9)
P 		193.5
(-0.9)
P 		235
avr 257.4
(3) 		195.7
(0.2) 		130.4
(-2.8) 		125.4
(-3) 		157.3
(-1.6) 		163.5
(-1.3) 		132
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177762_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17702
Sequence Type Exemplar sequence
Representative Public ID T22H6.5 NCBI
Target Description T22H6.5 /REP_DB=WormBase Gene ID /WP=CE03700 /TR=Q22690 /GB=CAA90673.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077735(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077735 NCBI Caenorhabditis elegans AntiBacterial Factor related family member (abf-5) (abf-5) mRNA, complete cds. 11/11 None
SNAP00000019110 ENSEMBL cdna:SNAP chromosome:CEL140:X:12792783:12793226:1 11/11 None
T22H6.5 ENSEMBL cdna:known chromosome:CEL140:X:12792768:12793338:1 gene:T22H6.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12792782-12793226(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol abf-5
Ensembl T22H6.5
Entrez Gene 188770 Entrez gene
SwissProt Q22690 EMBL-EBI
WormBase CE03700 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90673 Hypothetical protein T22H6.5 [Caenorhabditis elegans] ref|NP_510136.1| AntiBacterial Factor related family member (abf-5) [Caenorhabditis elegans] dbj|BAC44992.1| ABF-5 [Caenorhabditis elegans] 2.0E-76
blast CAE57212 Hypothetical protein CBG00070 [Caenorhabditis briggsae] 3.0E-55
blast CAB07627 Hypothetical protein F54B8.5 [Caenorhabditis elegans] ref|NP_506950.1| AntiBacterial Factor related family member (abf-3) [Caenorhabditis elegans] 6.0E-30
Sequence
Target Sequence 
>C. ELEGANS:177762_AT
ctataactttttgctgctttccgcctgtatcatttttctgattcctgaaaaatctgaaag
tatttgtgtaacacgaagaaccgactgggggcagtttggatcgtttttcaccgatcctct
ttgtgatgtttggtgcaggattcgaagatgcggaaaaggccaatgcagagaagacccagc
gacgtcaaacacagctaattgcgtttgcgagaagtgttatagagatgacgacggaaacgt
tatcttcccagataatgatggtttccaacaatcgcgtctgaactttgataatagccccac
atcgtcgtcgccttggacaatgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTATAACTTTTTGCTGCTTTCCGCC 411 223 18 Antisense
TTTCCGCCTGTATCATTTTTCTGAT 76 671 35 Antisense
ACACGAAGAACCGACTGGGGGCAGT 178 71 88 Antisense
GGGCAGTTTGGATCGTTTTTCACCG 701 489 106 Antisense
CACCGATCCTCTTTGTGATGTTTGG 113 201 126 Antisense
GAGAAGACCCAGCGACGTCAAACAC 471 389 185 Antisense
AACACAGCTAATTGCGTTTGCGAGA 231 135 205 Antisense
GACGACGGAAACGTTATCTTCCCAG 389 375 244 Antisense
GGTTTCCAACAATCGCGTCTGAACT 247 505 277 Antisense
ACTTTGATAATAGCCCCACATCGTC 240 103 299 Antisense
CATCGTCGTCGCCTTGGACAATGAA 537 211 317 Antisense
Sequence Source Affymetrix Proprietary Database