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[177760_at]

Description W01B6.9 /REP_DB=WormBase Gene ID /WP=CE06537 /TR=Q17635 /GB=CAA92629.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 87      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_highest_nonorm > 92      6grp_total > 77      4grp_total > 37      6grp_highest_sub > In      6grp_cgc > 58      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 152.6
(0.6)
P 		178.4
(1.7)
P 		67.9
(-3)
P 		208
(3)
P 		140
B 134.2
(1.9)
P 		131.9
(1.6)
P 		142.9
(3)
P 		96.6
(-3)
P 		46
C 202.7
(3)
P 		147.9
(-0.9)
P 		122.7
(-2.7)
P 		119.1
(-3)
P 		84
avr 163.2
(3) 		152.7
(1.8) 		111.2
(-3) 		141.2
(0.5) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 678
1 2 3 4 5 6 range
A 152.5
(-2.4)
P 		124.8
(-3)
P 		255.1
(-0.2)
P 		185
(-1.7)
P 		277.1
(0.2)
P 		407.3
(3)
P 		283
B 121.6
(-2.9)
P 		113.7
(-3)
P 		128.2
(-2.9)
P 		231.4
(-2)
P 		372.8
(-0.7)
P 		792
(3)
P 		678
C 135.2
(-2.9)
P 		167.7
(-2.5)
P 		122.8
(-3)
P 		283.5
(-1.3)
P 		238.7
(-1.8)
P 		684.7
(3)
P 		562
avr 136.4
(-3) 		135.4
(-3) 		168.7
(-2.6) 		233.3
(-1.8) 		296.2
(-1) 		628
(3) 		493
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 55.6
(3)
P 		38.7
(0.3)
P 		17.7
(-3)
P 		30.5
(-1)
P 		38
B 42.8
(3)
P 		32.6
(0.2)
P 		33.1
(0.4)
P 		20.6
(-3)
P 		22
C 67.1
(3)
P 		42.5
(-0.1)
P 		42.8
(-0.1)
P 		20.1
(-3)
P 		47
avr 55.2
(3) 		37.9
(-0.3) 		31.2
(-1.6) 		23.7
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 267
1 2 3 4 5 6 range
A 28.1
(-3)
P 		44.4
(-1.6)
P 		55.6
(-0.7)
P 		46.9
(-1.4)
P 		83.6
(1.7)
P 		99.2
(3)
P 		71
B 42.3
(-2.7)
P 		37.8
(-2.8)
P 		31.1
(-3)
P 		61.3
(-2.3)
P 		151.5
(-0.3)
P 		295.3
(3)
P 		264
C 50.1
(-2.4)
P 		51.2
(-2.4)
P 		31.3
(-3)
P 		57
(-2.2)
P 		77.2
(-1.6)
P 		229.2
(3)
P 		198
avr 40.2
(-3) 		44.5
(-2.8) 		39.3
(-3) 		55.1
(-2.4) 		104.1
(-0.7) 		207.9
(3) 		169
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177760_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10128
Sequence Type Exemplar sequence
Representative Public ID W01B6.9 NCBI
Target Description W01B6.9 /REP_DB=WormBase Gene ID /WP=CE06537 /TR=Q17635 /GB=CAA92629.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069429(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069429 NCBI Caenorhabditis elegans Yeast NDC (nuclear division cycle) homolog family member (ndc-80) (ndc-80) mRNA, complete cds. 11/11 None
SNAP00000040129 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10086886:10088282:1 11/11 None
GENEFINDER00000040148 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10086956:10088282:1 11/11 None
W01B6.9.2 ENSEMBL cdna:known chromosome:CEL140:IV:10086107:10088282:1 gene:W01B6.9 11/11 None
W01B6.9.1 ENSEMBL cdna:known chromosome:CEL140:IV:10086109:10088397:1 gene:W01B6.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10086124-10088288(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ndc-80
Ensembl W01B6.9
Entrez Gene 177873 Entrez gene
SwissProt Q17635 EMBL-EBI
WormBase CE06537 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251844_AT expressed protein at
CANINE_2:CFA.3066.1.S1_AT similar to kinetochore associated 2 cfa
CANINE_2:CFA.3066.1.S1_S_AT similar to kinetochore associated 2 cfa
CANINE_2:CFAAFFX.28162.1.S1_AT similar to kinetochore associated 2 cfa
CHICKEN:GGA.9350.1.S1_S_AT kinetochore associated 2 gga
CHICKEN:GGAAFFX.11749.1.S1_AT kinetochore associated 2 gga
HG-U133_PLUS_2:204162_AT kinetochore associated 2 hs
HG-U133A:204162_AT kinetochore associated 2 hs
HG-FOCUS:204162_AT kinetochore associated 2 hs
HG-U133A_2:204162_AT kinetochore associated 2 hs
HU35KSUBB:RC_AA188981_AT kinetochore associated 2 hs
U133_X3P:G5174456_3P_AT kinetochore associated 2 hs
HG-U95AV2:40041_AT kinetochore associated 2 hs
MU11KSUBA:C77976_RC_AT kinetochore associated 2 mm
MU11KSUBA:AA267955_S_AT kinetochore associated 2 mm
MG-U74CV2:129983_AT kinetochore associated 2 mm
MOE430A:1417445_AT kinetochore associated 2 mm
MOUSE430A_2:1417445_AT kinetochore associated 2 mm
MOUSE430_2:1417445_AT kinetochore associated 2 mm
YEAST_2:1778888_AT Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Sc
Protein Similarities
Method ID Description E-Value
blast CAA94680 Hypothetical protein W01B6.9 [Caenorhabditis elegans] emb|CAA92629.1| Hypothetical protein W01B6.9 [Caenorhabditis elegans] ref|NP_501830.1| Yeast NDC (nuclear division cycle) homolog family member (ndc-80) [Caenorhabditis elegans] 0.0
blast CAE75255 Hypothetical protein CBG23215 [Caenorhabditis briggsae] 0.0
blast CAA94680 Hypothetical protein W01B6.9 [Caenorhabditis elegans] emb|CAA92629.1| Hypothetical protein W01B6.9 [Caenorhabditis elegans] ref|NP_501830.1| Yeast NDC (nuclear division cycle) homolog family member (ndc-80) [Caenorhabditis elegans] 1.0E-180
blast CAE75255 Hypothetical protein CBG23215 [Caenorhabditis briggsae] 1.0E-105
blast CAE75255 Hypothetical protein CBG23215 [Caenorhabditis briggsae] 4.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR005550 EMBL-EBI HEC/Ndc80p family 2.3E-121
Sequence
Target Sequence 
>C. ELEGANS:177760_AT
agtccaaattcattcatctcgcagaacaaatcatgaaaattctcagtggacttaatattc
aaatgaatcttgaaccactaagagctccaacgaatgaaagagatcttaaggattactggg
agacgttgaacaagatatgggttccagagatcagccgacagttgcatcagagaaagcttg
agcttgagactgaacagtcaagattttcaaataaagcagttaccgcagaagaaagaatcc
aaattcaaagcgaaacactctgtgaagcgaagaagaatgaggctcgcgaagaacgaattc
gtcgaaacgaacgtgatagctggaaagatgctcgcaaacatattgaacaacgatatgaac
aactattgaatgaaaaagaagtacttctaaagcaaatgaaacttgatggaagcctcgaga
aagaaatcgaagatgagaccgccagaatgtctgcaactggagaagaacacatacaaaaga
gatctcaattggaagctggaatccggcaaatattggatctgatggtagtggaaatcgccg
agatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTCCAAATTCATTCATCTCGCAGA 127 61 1181 Antisense
GAACCACTAAGAGCTCCAACGAATG 479 345 1252 Antisense
GGATTACTGGGAGACGTTGAACAAG 221 287 1290 Antisense
TATGGGTTCCAGAGATCAGCCGACA 518 655 1316 Antisense
ATCAGCCGACAGTTGCATCAGAGAA 320 29 1330 Antisense
AGCTTGAGCTTGAGACTGAACAGTC 507 77 1355 Antisense
GAGGCTCGCGAAGAACGAATTCGTC 170 405 1459 Antisense
GATAGCTGGAAAGATGCTCGCAAAC 163 425 1495 Antisense
GAGACCGCCAGAATGTCTGCAACTG 300 393 1615 Antisense
GGAAGCTGGAATCCGGCAAATATTG 87 529 1671 Antisense
GATGGTAGTGGAAATCGCCGAGATT 420 407 1701 Antisense
Sequence Source Affymetrix Proprietary Database