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[177746_at]

Description C32C4.3 /REP_DB=WormBase Gene ID /WP=CE05346 /TR=Q18353 /GB=CAA98111.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 36      4grp_total > 20      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 200
1 2 3 4 range
A 86.1
(-0.6)
A 		86.3
(-0.6)
A 		6.7
(-3)
A 		206.5
(3)
A 		200
B 64.8
(-3)
A 		98.6
(1.1)
A 		77.3
(-1.5)
A 		114.2
(3)
A 		49
C 87
(-1.2)
A 		69.7
(-2.1)
A 		51.5
(-3)
A 		167.6
(3)
P 		116
avr 79.3
(-1.3) 		84.9
(-1) 		45.2
(-3) 		162.8
(3) 		118

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 151.2
(3)
A 		68.7
(-2.2)
A 		89.4
(-0.9)
A 		56.9
(-3)
A 		64.4
(-2.5)
A 		72.8
(-2)
A 		94
B 103.1
(2.6)
A 		104.1
(2.7)
A 		49.7
(-3)
A 		63.8
(-1.5)
A 		77.9
(-0)
A 		106.6
(3)
P 		57
C 47.5
(-3)
A 		170.7
(3)
M 		58.1
(-2.5)
A 		65.5
(-2.1)
A 		64
(-2.2)
A 		103
(-0.3)
A 		123
avr 100.6
(1.4) 		114.5
(3) 		65.7
(-2.6) 		62.1
(-3) 		68.8
(-2.2) 		94.1
(0.7) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 31.3
(3)
A 		18.7
(0.4)
A 		1.7
(-3)
A 		30.2
(2.8)
A 		30
B 20.7
(-0.4)
A 		24.3
(3)
A 		17.9
(-3)
A 		24.3
(3)
A 		6
C 28.8
(3)
A 		20
(-1.8)
A 		17.9
(-3)
A 		28.3
(2.7)
P 		11
avr 26.9
(2.7) 		21
(0.4) 		12.5
(-3) 		27.6
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 27.9
(3)
A 		24.4
(1.4)
A 		19.5
(-0.7)
A 		14.4
(-3)
A 		19.4
(-0.8)
A 		17.7
(-1.5)
A 		14
B 35.9
(2.2)
A 		34.6
(1.9)
A 		12.1
(-3)
A 		16.9
(-2)
A 		31.7
(1.3)
A 		39.7
(3)
P 		28
C 17.6
(-2.3)
A 		52.1
(3)
M 		14.8
(-2.8)
A 		13.2
(-3)
A 		20.7
(-1.8)
A 		34.5
(0.3)
A 		39
avr 27.1
(0.3) 		37
(3) 		15.5
(-2.8) 		14.8
(-3) 		23.9
(-0.5) 		30.6
(1.3) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177746_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13176
Sequence Type Exemplar sequence
Representative Public ID C32C4.3 NCBI
Target Description C32C4.3 /REP_DB=WormBase Gene ID /WP=CE05346 /TR=Q18353 /GB=CAA98111.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073325(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073325 NCBI Caenorhabditis elegans C32C4.3 (C32C4.3) mRNA, complete cds. 9/11 None
SNAP00000003072 ENSEMBL cdna:SNAP chromosome:CEL140:V:10647724:10649662:-1 9/11 None
GENEFINDER00000003076 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10647724:10649512:-1 9/11 None
C32C4.3 ENSEMBL cdna:known chromosome:CEL140:V:10647741:10649512:-1 gene:C32C4.3 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10647571-10649512(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32C4.3
Entrez Gene 183122 Entrez gene
SwissProt Q18353 EMBL-EBI
WormBase CE37878 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98111 Hypothetical protein C32C4.3 [Caenorhabditis elegans] ref|NP_505726.2| C32C4.3 [Caenorhabditis elegans] 0.0
blast CAA98111 Hypothetical protein C32C4.3 [Caenorhabditis elegans] ref|NP_505726.2| C32C4.3 [Caenorhabditis elegans] 1.0E-174
blast CAA98111 Hypothetical protein C32C4.3 [Caenorhabditis elegans] ref|NP_505726.2| C32C4.3 [Caenorhabditis elegans] 1.0E-158
blast CAE64864 Hypothetical protein CBG09662 [Caenorhabditis briggsae] 4.0E-44
blast CAE64864 Hypothetical protein CBG09662 [Caenorhabditis briggsae] 4.0E-38
blast CAE64864 Hypothetical protein CBG09662 [Caenorhabditis briggsae] 4.0E-33
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505726.2 1 242-264
C32C4.3 1 242-264
SNAP00000003072 1 227-249
GENEFINDER00000003076 1 242-264
Sequence
Target Sequence 
>C. ELEGANS:177746_AT
gaatttctgactacttgtggacctcatcaactggatccgctgcttttgagtattagttcg
gaaaaattggagacaattgtggacagttttgcatctttgagttcagcatttctcaatatc
tccgatggaattgagccgaagtatatgtacttttcatcggttccgaccagttataccccg
atttggccatctcttgtagcagtttttgtagttctcatattctttgtcatggggctcatt
ctctgttgttgtggccttttcatgtttggattggtagaggaggatttccagaaacgaaac
gaagaaaatgagtggttggaagcaaatcgtaggcgagaagagtatttgattaatgcacat
gaagatccaattgaaattccgaaccttcataatcaggataactcaagatcatcagaatcg
gatatgcttccacatacagccaagagtcacaacagcatggtgagctccacttcaagtacc
gctgttgttactgacagaactcaaatgtccgaaactactatatattgttcaccgagactg
ttgtattccgccgaacttttcttcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTTCTGACTACTTGTGGACCTC 331 325 421 Antisense
TGTGGACCTCATCAACTGGATCCGC 333 555 436 Antisense
GGATCCGCTGCTTTTGAGTATTAGT 376 511 453 Antisense
GTTCAGCATTTCTCAATATCTCCGA 401 445 521 Antisense
TCATCGGTTCCGACCAGTTATACCC 226 617 574 Antisense
GATTTGGCCATCTCTTGTAGCAGTT 692 425 600 Antisense
GGGCTCATTCTCTGTTGTTGTGGCC 478 493 652 Antisense
GTTGTGGCCTTTTCATGTTTGGATT 372 437 668 Antisense
CAGCATGGTGAGCTCCACTTCAAGT 456 203 873 Antisense
GTTCACCGAGACTGTTGTATTCCGC 251 445 947 Antisense
TGTATTCCGCCGAACTTTTCTTCGA 55 561 962 Antisense
Sequence Source Affymetrix Proprietary Database