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[177745_s_at]

Description C28A5.2 /REP_DB=WormBase Gene ID /WP=CE00676 /TR=Q18270 /GB=CAA83597.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 77      4grp_total > 0      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 18.4
(-1.6)
A 		6.7
(-2.9)
A 		5.9
(-3)
A 		58.7
(3)
A 		53
B 12.1
(3)
A 		7.3
(-0.8)
A 		6.1
(-1.8)
A 		4.6
(-3)
A 		8
C 36
(-0.2)
A 		12.2
(-3)
A 		51
(1.6)
A 		62.8
(3)
A 		51
avr 22.2
(-0.6) 		8.7
(-3) 		21
(-0.8) 		42
(3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 176
1 2 3 4 5 6 range
A 18.8
(-3)
A 		28.4
(-2.4)
A 		28.3
(-2.4)
A 		28.5
(-2.4)
A 		59.6
(-0.4)
A 		114.6
(3)
P 		96
B 32.3
(-2)
A 		3.5
(-3)
A 		41.6
(-1.7)
A 		55.3
(-1.2)
A 		73.3
(-0.6)
A 		179.3
(3)
P 		176
C 21.9
(-2.4)
A 		48.3
(-1.5)
A 		5.4
(-3)
A 		36.9
(-1.9)
A 		58.3
(-1.1)
A 		176.9
(3)
P 		172
avr 24.3
(-3) 		26.7
(-2.9) 		25.1
(-3) 		40.2
(-2.3) 		63.7
(-1.2) 		156.9
(3) 		133
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 6.7
(1.4)
A 		1.5
(-3)
A 		1.5
(-3)
A 		8.6
(3)
A 		7
B 3.9
(3)
A 		1.8
(-1.3)
A 		1.4
(-2.2)
A 		1
(-3)
A 		3
C 11.9
(0.5)
A 		3.5
(-3)
A 		17.8
(3)
A 		10.6
(-0)
A 		14
avr 7.5
(3) 		2.3
(-3) 		6.9
(2.3) 		6.7
(2.1) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 3.5
(-3)
A 		10.1
(-1.4)
A 		6.2
(-2.3)
A 		7.2
(-2.1)
A 		18
(0.6)
A 		27.9
(3)
P 		24
B 11.2
(-2.1)
A 		1.2
(-3)
A 		10.1
(-2.2)
A 		14.7
(-1.8)
A 		29.8
(-0.4)
A 		66.9
(3)
P 		66
C 8.1
(-2.3)
A 		14.7
(-1.6)
A 		1.4
(-3)
A 		7.4
(-2.4)
A 		18.9
(-1.2)
A 		59.2
(3)
P 		58
avr 7.6
(-2.8) 		8.7
(-2.6) 		5.9
(-3) 		9.8
(-2.5) 		22.2
(-0.8) 		51.3
(3) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177745_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6296
Sequence Type Exemplar sequence
Representative Public ID C28A5.2 NCBI
Target Description C28A5.2 /REP_DB=WormBase Gene ID /WP=CE00676 /TR=Q18270 /GB=CAA83597.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065501(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065501 NCBI Caenorhabditis elegans C28A5.2 (C28A5.2) mRNA, complete cds. 11/11 None
SNAP00000011688 ENSEMBL cdna:SNAP chromosome:CEL140:III:4429693:4439512:-1 11/11 None
GENEFINDER00000011693 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4434833:4439512:-1 11/11 None
C28A5.2 ENSEMBL cdna:known chromosome:CEL140:III:4434730:4439512:-1 gene:C28A5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4434832-4439512(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C28A5.2
Entrez Gene 3565701 Entrez gene
SwissProt Q18270 EMBL-EBI
WormBase CE00676 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83597 Hypothetical protein C28A5.2 [Caenorhabditis elegans] ref|NP_497902.1| C28A5.2 [Caenorhabditis elegans] 0.0
blast CAA83599 Hypothetical protein C28A5.1 [Caenorhabditis elegans] ref|NP_497901.1| C28A5.1 [Caenorhabditis elegans] 0.0
blast CAE63693 Hypothetical protein CBG08207 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:177745_S_AT
atactgttccacttcccagattaaaggaaaaaactgacaaggttgtgccacagataagaa
cagaaccggtgcaaagtggcactttttctgtggacgactacggatgttgggaaactttac
gaatcaattcgattttggctcaagagaagcttgacagaatgcgggccgttggcgatccaa
gagttaagatgattgacggaccagatttgagaccagaaatcgaggttcgaaaggtcgatg
tgaatacgagacgagagaaaatgttcgcagcgtttgatgcacttctggagaagttgccac
aacttcacagcgaaggaattctcggatacgtcctggaggatgatttacgagtaaagatta
aaaaattgacgccaatgaacgataataatcataaatttacattcacattcagaaacggct
cgctggaaatcggtgtgaattacctcacggatatgctcactagaggaaaaatatacaaag
tgatgaatgagatgaaaaaacatgttcctctgctta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATACTGTTCCACTTCCCAGATTAAA 43 25 3044 Antisense
AAACTGACAAGGTTGTGCCACAGAT 160 127 3074 Antisense
AGTGGCACTTTTTCTGTGGACGACT 269 59 3118 Antisense
GTGGACGACTACGGATGTTGGGAAA 523 489 3133 Antisense
GAAGCTTGACAGAATGCGGGCCGTT 594 341 3189 Antisense
GGGCCGTTGGCGATCCAAGAGTTAA 404 491 3206 Antisense
GACGGACCAGATTTGAGACCAGAAA 102 377 3238 Antisense
CATTCACATTCAGAAACGGCTCGCT 538 215 3443 Antisense
AACGGCTCGCTGGAAATCGGTGTGA 602 143 3457 Antisense
ATCGGTGTGAATTACCTCACGGATA 513 35 3472 Antisense
ATGAAAAAACATGTTCCTCTGCTTA 323 43 3535 Antisense
Sequence Source Affymetrix Proprietary Database