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[177741_s_at]

Description ZK863.4 /REP_DB=WormBase Gene ID /WP=CE23469 /TR=Q23652 /GB=CAB01456.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 86      4grp_highest_8_8 > 53      4grp_high_8_8 > 17      6grp_highest_nonorm > 94      6grp_total > 76      4grp_total > 23      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 265
1 2 3 4 range
A 512.5
(3)
P 		354.1
(-2.4)
P 		337.9
(-3)
P 		413.3
(-0.4)
P 		175
B 396.5
(3)
P 		327.5
(-3)
P 		340.5
(-1.9)
P 		377.5
(1.3)
P 		69
C 394.1
(3)
P 		290.1
(-1.3)
P 		247.8
(-3)
P 		377.7
(2.3)
P 		146
avr 434.4
(3) 		323.9
(-2.3) 		308.7
(-3) 		389.5
(0.9) 		126

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 670
1 2 3 4 5 6 range
A 534
(-2.1)
P 		447.4
(-3)
P 		533.8
(-2.2)
P 		637.6
(-1.1)
P 		618.7
(-1.3)
P 		1058.2
(3)
P 		611
B 401.7
(-3)
P 		401.1
(-3)
P 		522.3
(-1.9)
P 		558.9
(-1.6)
P 		561.9
(-1.6)
P 		1071.1
(3)
P 		670
C 407.6
(-3)
P 		538.4
(-1.3)
P 		490.7
(-2)
P 		779.9
(1.7)
P 		638
(-0.1)
P 		882.7
(3)
P 		475
avr 447.8
(-3) 		462.3
(-2.8) 		515.6
(-2.3) 		658.8
(-0.7) 		606.2
(-1.3) 		1004
(3) 		556
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 186.6
(3)
P 		76.9
(-2.2)
P 		88.3
(-1.7)
P 		60.5
(-3)
P 		126
B 126.4
(3)
P 		80.9
(-2.7)
P 		78.9
(-3)
P 		80.4
(-2.8)
P 		48
C 130.5
(3)
P 		83.4
(-1.2)
P 		86.4
(-1)
P 		63.7
(-3)
P 		67
avr 147.8
(3) 		80.4
(-2.1) 		84.5
(-1.8) 		68.2
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 301
1 2 3 4 5 6 range
A 98.4
(-3)
P 		159.1
(-0.7)
P 		116.2
(-2.3)
P 		161.7
(-0.6)
P 		186.7
(0.3)
P 		257.6
(3)
P 		159
B 139.8
(-2.7)
P 		133.5
(-2.9)
P 		126.8
(-3)
P 		148
(-2.5)
P 		228.3
(-0.8)
P 		399.4
(3)
P 		273
C 151.1
(-2.1)
P 		164.3
(-1.6)
P 		124.9
(-3)
P 		156.8
(-1.9)
P 		206.4
(-0.1)
P 		295.5
(3)
P 		171
avr 129.8
(-2.8) 		152.3
(-2.1) 		122.6
(-3) 		155.5
(-2) 		207.1
(-0.4) 		317.5
(3) 		195
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177741_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13567
Sequence Type Exemplar sequence
Representative Public ID ZK863.4 NCBI
Target Description ZK863.4 /REP_DB=WormBase Gene ID /WP=CE23469 /TR=Q23652 /GB=CAB01456.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073655(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073655 NCBI Caenorhabditis elegans ZK863.4 (ZK863.4) mRNA, complete cds. 11/11 None
SNAP00000039742 ENSEMBL cdna:SNAP chromosome:CEL140:V:12184333:12186763:-1 11/11 None
GENEFINDER00000039748 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12184333:12186763:-1 11/11 None
ZK863.4.1 ENSEMBL cdna:known chromosome:CEL140:V:12184100:12186773:-1 gene:ZK863.4 11/11 None
ZK863.4.2 ENSEMBL cdna:known chromosome:CEL140:V:12184110:12186782:-1 gene:ZK863.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12184332-12186763(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK863.4
Entrez Gene 179670 Entrez gene
SwissProt Q23652 EMBL-EBI
WormBase CE23469 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01456 Hypothetical protein ZK863.4 [Caenorhabditis elegans] ref|NP_506056.1| ZK863.4 [Caenorhabditis elegans] 0.0
blast CAE75410 Hypothetical protein CBG23400 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002058 EMBL-EBI PAP/25A-associated domain 1.1E-14
Sequence
Target Sequence 
>C. ELEGANS:177741_S_AT
gccgagcaccagtacttgtcagtacaacttccagccgtggtgataacctcgagtgatctt
ttcctccttcttactagagttcttcgctttaatgtctgtccgaatgagcaaggacccagt
gttgttgatttatgtactccaacgggtgccattttccttgtaacttcaagagcttgggtt
ggacgtcgcaatacgaaacgcgctttgaaaaactctcgtcacgatttgacgcctctccag
attgatgttatgtgcagtgacaagtacgattacgaagaagatattgcggagttgcgtatc
tcgatgagcactgttcctggcaccagaattaggttggcatgcatcgatataatgcgtggt
caagtttccgaagttcgcgacgcaatccact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCGAGCACCAGTACTTGTCAGTAC 609 283 1510 Antisense
CAGTACAACTTCCAGCCGTGGTGAT 557 207 1529 Antisense
ATAACCTCGAGTGATCTTTTCCTCC 368 25 1552 Antisense
TTTTCCTCCTTCTTACTAGAGTTCT 382 677 1568 Antisense
GAGTTCTTCGCTTTAATGTCTGTCC 140 399 1586 Antisense
TTATGTACTCCAACGGGTGCCATTT 71 663 1639 Antisense
GAGCTTGGGTTGGACGTCGCAATAC 519 387 1679 Antisense
AACTCTCGTCACGATTTGACGCCTC 246 139 1720 Antisense
TTGACGCCTCTCCAGATTGATGTTA 347 705 1735 Antisense
TATCTCGATGAGCACTGTTCCTGGC 4 659 1806 Antisense
TCCGAAGTTCGCGACGCAATCCACT 125 595 1876 Antisense
Sequence Source Affymetrix Proprietary Database