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[177739_s_at]

Description C28A5.1 /REP_DB=WormBase Gene ID /WP=CE00675 /TR=Q18271 /GB=CAA83599.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 88      4grp_high_8_8 > 19      6grp_total > 87      4grp_total > 28      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 110.8
(-1.2)
P 		140.2
(3)
P 		98.4
(-3)
P 		129.9
(1.5)
P 		42
B 126.8
(0.9)
A 		139.3
(3)
P 		103.8
(-3)
M 		111.9
(-1.6)
P 		36
C 157.4
(0.7)
P 		127.2
(-1.6)
P 		108
(-3)
P 		188.2
(3)
P 		80
avr 131.7
(1.3) 		135.6
(1.8) 		103.4
(-3) 		143.3
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 654
1 2 3 4 5 6 range
A 62.6
(-3)
A 		136.7
(-2.2)
P 		189.7
(-1.6)
P 		185.7
(-1.7)
P 		350.8
(0.1)
P 		620.7
(3)
P 		558
B 118.7
(-3)
P 		128.6
(-2.9)
P 		171.2
(-2.4)
P 		166.4
(-2.5)
P 		361.3
(-0.3)
P 		653.6
(3)
P 		535
C 129.3
(-3)
P 		156.4
(-2.7)
P 		138.2
(-2.9)
P 		216.3
(-2.1)
P 		406.4
(-0.2)
P 		717
(3)
P 		588
avr 103.5
(-3) 		140.6
(-2.6) 		166.4
(-2.3) 		189.5
(-2.1) 		372.8
(-0.1) 		663.8
(3) 		560
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 40.3
(3)
P 		30.4
(0.2)
P 		25.7
(-1.1)
P 		19
(-3)
P 		21
B 40.4
(3)
A 		34.4
(0.8)
P 		24
(-3)
M 		23.9
(-3)
P 		17
C 52.1
(3)
P 		36.6
(-1.6)
P 		37.6
(-1.3)
P 		31.7
(-3)
P 		20
avr 44.3
(3) 		33.8
(-0.2) 		29.1
(-1.7) 		24.9
(-3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 232
1 2 3 4 5 6 range
A 11.5
(-3)
A 		48.6
(-1.4)
P 		41.3
(-1.7)
P 		47.1
(-1.5)
P 		105.8
(1.1)
P 		151.1
(3)
P 		140
B 41.3
(-3)
P 		42.8
(-3)
P 		41.6
(-3)
P 		44.1
(-2.9)
P 		146.8
(0.1)
P 		243.7
(3)
P 		202
C 47.9
(-2.6)
P 		47.7
(-2.6)
P 		35.2
(-3)
P 		43.5
(-2.8)
P 		131.5
(-0.2)
P 		240
(3)
P 		205
avr 33.6
(-3) 		46.4
(-2.6) 		39.4
(-2.8) 		44.9
(-2.6) 		128
(0.2) 		211.6
(3) 		178
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177739_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6193
Sequence Type Exemplar sequence
Representative Public ID C28A5.1 NCBI
Target Description C28A5.1 /REP_DB=WormBase Gene ID /WP=CE00675 /TR=Q18271 /GB=CAA83599.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065500(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065500 NCBI Caenorhabditis elegans C28A5.1 (C28A5.1) mRNA, complete cds. 11/11 None
SNAP00000011688 ENSEMBL cdna:SNAP chromosome:CEL140:III:4429693:4439512:-1 11/11 None
GENEFINDER00000011695 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4429693:4434313:-1 11/11 None
C28A5.1 ENSEMBL cdna:known chromosome:CEL140:III:4429584:4434313:-1 gene:C28A5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4429692-4434313(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C28A5.1
Entrez Gene 175580 Entrez gene
SwissProt Q18271 EMBL-EBI
WormBase CE00675 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83599 Hypothetical protein C28A5.1 [Caenorhabditis elegans] ref|NP_497901.1| C28A5.1 [Caenorhabditis elegans] 0.0
blast CAA83597 Hypothetical protein C28A5.2 [Caenorhabditis elegans] ref|NP_497902.1| C28A5.2 [Caenorhabditis elegans] 0.0
blast CAE63693 Hypothetical protein CBG08207 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:177739_S_AT
atactgttccattgcccagattgattataaaatctaaaatctatgtgccaccgccacctg
tcgtcccggtttatgttaccccattcactgtaaacgactttggatgctgggaaacacatc
gtatcaattcgattctgcatcaggcgagaattgacagacttcgtgctgcaggccatatga
gagtcaaaatgcttgatggaccagatatgaaaccagaaatcgaaatccgaaaagtcgatg
tgaatacgagacgagagaaaatgttcacagcgtttgatgcacttctggagaagttgccac
aacttcacagcgaaggaattctcgaatacgtcctggaggatgatttgcgagaaaagatcg
aatatttgttgccaatgaccgatgataatcataaattccagttcagagcaagaaacgact
cgcttacaattggtctgaactacctcacggatatgctgaccaaagggaaagtatataaga
tgttgtacgagaaacaagaatatgttcctctgcttaaggtgttgtcagattgcatcggtc
tcaatacaacttatatcaaaaaaactgatgcagtgaccatgatgctgcaattcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATACTGTTCCATTGCCCAGATTGAT 471 23 3059 Antisense
ATCTAAAATCTATGTGCCACCGCCA 99 33 3090 Antisense
ACCCCATTCACTGTAAACGACTTTG 235 87 3136 Antisense
AACGACTTTGGATGCTGGGAAACAC 130 143 3151 Antisense
GTATCAATTCGATTCTGCATCAGGC 545 453 3179 Antisense
ATTGACAGACTTCGTGCTGCAGGCC 269 5 3208 Antisense
GTGCTGCAGGCCATATGAGAGTCAA 274 479 3221 Antisense
ATTTGTTGCCAATGACCGATGATAA 628 17 3422 Antisense
AACGACTCGCTTACAATTGGTCTGA 603 143 3472 Antisense
TGGTCTGAACTACCTCACGGATATG 554 549 3489 Antisense
CAGTGACCATGATGCTGCAATTCAT 567 207 3629 Antisense
Sequence Source Affymetrix Proprietary Database