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[177734_at]

Description R10E9.3 /REP_DB=WormBase Gene ID /WP=CE02302 /TR=Q20115 /GB=CAA84665.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 82      4grp_total > 03      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 161
1 2 3 4 range
A 150.4
(-2.3)
A 		169.7
(-1.4)
A 		134.8
(-3)
A 		269.1
(3)
P 		134
B 145.7
(-0.8)
M 		108.6
(-3)
A 		129.4
(-1.7)
A 		207.7
(3)
P 		99
C 148.7
(-1.5)
M 		111.8
(-3)
A 		141.5
(-1.8)
A 		257.2
(3)
P 		145
avr 148.3
(-2) 		130
(-3) 		135.2
(-2.7) 		244.7
(3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 183
1 2 3 4 5 6 range
A 116.1
(-1.8)
M 		125.8
(-1.4)
A 		184
(1.1)
P 		194
(1.6)
P 		227.1
(3)
P 		88.8
(-3)
A 		138
B 90.2
(-3)
A 		172.4
(-0)
P 		215.6
(1.6)
P 		202.5
(1.1)
P 		254.8
(3)
P 		174.6
(0.1)
P 		165
C 71.6
(-3)
A 		199.7
(1.6)
P 		114.6
(-1.5)
A 		239.7
(3)
P 		213.4
(2.1)
P 		109.1
(-1.7)
P 		168
avr 92.6
(-3) 		166
(0.2) 		171.4
(0.4) 		212.1
(2.2) 		231.8
(3) 		124.2
(-1.6) 		139
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 54.8
(3)
A 		36.8
(-2.5)
A 		35.2
(-3)
A 		39.4
(-1.7)
P 		20
B 46.5
(3)
M 		26.8
(-3)
A 		30
(-2)
A 		44.3
(2.3)
P 		20
C 49.2
(3)
M 		32.1
(-3)
A 		49.3
(3)
A 		43.4
(0.9)
P 		17
avr 50.2
(3) 		31.9
(-3) 		38.2
(-0.9) 		42.4
(0.4) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 21.4
(-3)
M 		44.7
(-0)
A 		40.1
(-0.6)
P 		49.2
(0.5)
P 		68.5
(3)
P 		21.6
(-3)
A 		47
B 31.4
(-3)
A 		57.4
(-0.8)
P 		52.3
(-1.3)
P 		53.6
(-1.2)
P 		103.5
(3)
P 		65.1
(-0.2)
P 		72
C 26.5
(-3)
A 		60.9
(1.8)
P 		29.2
(-2.6)
A 		48.2
(0.1)
P 		69.1
(3)
P 		36.5
(-1.6)
P 		43
avr 26.4
(-3) 		54.3
(0.1) 		40.5
(-1.4) 		50.3
(-0.3) 		80.4
(3) 		41.1
(-1.4) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177734_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6042
Sequence Type Exemplar sequence
Representative Public ID R10E9.3 NCBI
Target Description R10E9.3 /REP_DB=WormBase Gene ID /WP=CE02302 /TR=Q20115 /GB=CAA84665.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065396(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065396 NCBI Caenorhabditis elegans R10E9.3 (R10E9.3) mRNA, complete cds. 11/11 None
SNAP00000008641 ENSEMBL cdna:SNAP chromosome:CEL140:III:3960840:3961681:1 10/11 None
GENEFINDER00000008649 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3960840:3961681:1 10/11 None
R10E9.3 ENSEMBL cdna:known chromosome:CEL140:III:3960762:3961681:1 gene:R10E9.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3960760-3961681(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R10E9.3
Entrez Gene 187777 Entrez gene
SwissProt Q20115 EMBL-EBI
WormBase CE34086 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA84668 Hypothetical protein R10E9.3 [Caenorhabditis elegans] ref|NP_497797.2| R10E9.3 [Caenorhabditis elegans] 4.0E-70
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497797.2 4 7-29,34-56,63-85,111-133
R10E9.3 4 7-29,34-56,63-85,111-133
SNAP00000008641 4 7-29,34-56,63-85,111-133
GENEFINDER00000008649 4 7-29,34-56,63-85,111-133
Sequence
Target Sequence 
>C. ELEGANS:177734_AT
gacgatgctcggattcagctcatttttgattggagtcaaatctgttacttttgccattta
tttggtattgcttattgtatcaggtacggatctcagaatacagtttttttttttaatttt
cttcggagtttaaatcatttcgaaaaattagaaactttcgctgaatggcaaaattgtaat
tttaaatttaaattgaaactatatattccaattcaacataatttttcagtgatcctcctt
gacatttcatttttctacacaattcttgcatttggaatcgctgaatgctttctgatagct
atcacaatttatcatggaatcaagccagcgttgtacgtagtactcttaatgcttgctttt
gaaatgacgttgtcgttggtgaagttggtttttgcaattgttttgatggcaaaagatggt
ggaaatgattgttctgttgatcacccctgctcaattctacacatttcgctaattgaacga
ttttatttgttcttttttatactgatcaccgcatttttcgatggactagccgcacttttg
accatcgcgaattcaccacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGATGCTCGGATTCAGCTCATTT 338 379 88 Antisense
AGTGATCCTCCTTGACATTTCATTT 188 59 315 Antisense
GGAATCGCTGAATGCTTTCTGATAG 382 533 361 Antisense
GGAATCAAGCCAGCGTTGTACGTAG 664 525 403 Antisense
GTAGTACTCTTAATGCTTGCTTTTG 188 457 424 Antisense
AATGATTGTTCTGTTGATCACCCCT 140 165 511 Antisense
CTGCTCAATTCTACACATTTCGCTA 32 239 534 Antisense
GTTCTTTTTTATACTGATCACCGCA 28 445 576 Antisense
GATCACCGCATTTTTCGATGGACTA 161 421 591 Antisense
ATGGACTAGCCGCACTTTTGACCAT 5 51 608 Antisense
TTTGACCATCGCGAATTCACCACAA 81 669 624 Antisense
Sequence Source Affymetrix Proprietary Database