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[177731_at]

Description F54F7.6 /REP_DB=WormBase Gene ID /WP=CE03421 /TR=Q20793 /GB=CAA91759.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 69      4grp_total > 0      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 17.8
(-2.9)
A 		17.4
(-3)
A 		27.5
(-1.5)
A 		59.1
(3)
A 		42
B 26.6
(0.1)
A 		9.8
(-3)
A 		32.6
(1.2)
A 		42.6
(3)
A 		33
C 33.9
(0.1)
A 		16.7
(-2.9)
A 		16.2
(-3)
A 		50
(3)
A 		34
avr 26.1
(-1.1) 		14.6
(-3) 		25.4
(-1.2) 		50.6
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 71.4
(0.8)
A 		40.5
(-2.9)
A 		50.5
(-1.7)
A 		39.6
(-3)
A 		50
(-1.8)
A 		90.1
(3)
P 		51
B 23.6
(-2.6)
A 		61.9
(-0.6)
A 		15.6
(-3)
A 		34.9
(-2)
A 		60.3
(-0.6)
A 		129.7
(3)
P 		114
C 34.9
(-1.7)
A 		57.6
(-0.5)
A 		8.5
(-3)
A 		81
(0.7)
A 		50.1
(-0.9)
A 		126
(3)
P 		118
avr 43.3
(-1.8) 		53.3
(-1.1) 		24.9
(-3) 		51.8
(-1.2) 		53.5
(-1.1) 		115.3
(3) 		90
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 6.5
(0.3)
A 		3.8
(-3)
A 		7.2
(1.2)
A 		8.7
(3)
A 		5
B 8.5
(2.5)
A 		2.4
(-3)
A 		7.6
(1.7)
A 		9.1
(3)
A 		7
C 11.2
(3)
A 		4.8
(-3)
A 		5.6
(-2.3)
A 		8.4
(0.4)
A 		6
avr 8.7
(3) 		3.7
(-3) 		6.8
(0.7) 		8.7
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 13.2
(-1.4)
A 		14.4
(-0.8)
A 		11
(-2.5)
A 		10.1
(-3)
A 		15.1
(-0.5)
A 		21.9
(3)
P 		12
B 8.2
(-2.4)
A 		20.6
(-0.7)
A 		3.8
(-3)
A 		9.2
(-2.3)
A 		24.5
(-0.2)
A 		48.4
(3)
P 		45
C 13
(-1.4)
A 		17.6
(-0.7)
A 		2.2
(-3)
A 		16.3
(-0.9)
A 		16.2
(-0.9)
A 		42.2
(3)
P 		40
avr 11.5
(-1.9) 		17.5
(-0.8) 		5.7
(-3) 		11.9
(-1.8) 		18.6
(-0.6) 		37.5
(3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177731_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16356
Sequence Type Exemplar sequence
Representative Public ID F54F7.6 NCBI
Target Description F54F7.6 /REP_DB=WormBase Gene ID /WP=CE03421 /TR=Q20793 /GB=CAA91759.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077579(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077579 NCBI Caenorhabditis elegans F54F7.6 (F54F7.6) mRNA, complete cds. 11/11 None
GENEFINDER00000009571 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11863534:11866657:1 11/11 None
SNAP00000009562 ENSEMBL cdna:SNAP chromosome:CEL140:X:11865307:11866657:1 11/11 None
F54F7.6 ENSEMBL cdna:known chromosome:CEL140:X:11865463:11866657:1 gene:F54F7.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11865462-11866657(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54F7.6
Entrez Gene 186254 Entrez gene
SwissProt Q20793 EMBL-EBI
WormBase CE03421 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91759 Hypothetical protein F54F7.6 [Caenorhabditis elegans] ref|NP_509980.1| F54F7.6 [Caenorhabditis elegans] 1.0E-176
blast CAE56668 Hypothetical protein CBG24440 [Caenorhabditis briggsae] 2.0E-39
blast CAE61433 Hypothetical protein CBG05315 [Caenorhabditis briggsae] 2.0E-39
blast CAB54366 Hypothetical protein Y105C5B.20 [Caenorhabditis elegans] ref|NP_502909.1| Y105C5B.20 [Caenorhabditis elegans] 3.0E-33
Protein Domains
Database ID Description E-Value
Pfam IPR001232 EMBL-EBI SKP1 component 3.5E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509980.1 1 281-303
F54F7.6 1 281-303
GENEFINDER00000009571 1 432-454
SNAP00000009562 1 333-355
Sequence
Target Sequence 
>C. ELEGANS:177731_AT
agatttcattcttacaactcctgccaaacagtccaacgatagacactctttctactcaaa
gattgacactgaaatcgcatacaacacaattaacgaaattcagaaaactgcaaagaaaga
tgaaaatcataagctggaaagcgtgtattctagaagtactccattcacagaaattttttc
gaaaataaaacgtttaccaaaggagaaaggagtagatgttcaaatggttcaaaaaaatct
cgtgaaaaaggataatagaaaatcattaaaggatatggatgaaagtaaaagcccgaaacc
attcgagttggccgtttttaatcaggatggtgggatgttctcggtgtggatgactaaaga
gcaaatgcaaaaatatttgggaaaacagttggaagctgagcagaatgatcctgaaactga
cactcaagccttatccgattcgtccgactcaaatgatcaattcaaaaagattggctccat
tattgctataggactttttgcagttgttttccttttgtatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATTTCATTCTTACAACTCCTGCC 28 67 384 Antisense
AACTCCTGCCAAACAGTCCAACGAT 184 139 399 Antisense
AACGATAGACACTCTTTCTACTCAA 256 145 418 Antisense
GTATTCTAGAAGTACTCCATTCACA 292 453 528 Antisense
AGCCCGAAACCATTCGAGTTGGCCG 409 83 673 Antisense
GAGTTGGCCGTTTTTAATCAGGATG 132 395 688 Antisense
GTGGGATGTTCTCGGTGTGGATGAC 296 489 713 Antisense
GAAACTGACACTCAAGCCTTATCCG 656 357 796 Antisense
CTTATCCGATTCGTCCGACTCAAAT 522 221 813 Antisense
AGATTGGCTCCATTATTGCTATAGG 3 63 851 Antisense
TTTGCAGTTGTTTTCCTTTTGTATT 71 669 880 Antisense
Sequence Source Affymetrix Proprietary Database