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[177726_at]

Description K01G5.9 /REP_DB=WormBase Gene ID /WP=CE19950 /TR=Q9XTB8 /GB=CAB07249.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 38      4grp_total > 55      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 283
1 2 3 4 range
A 506
(3)
P 		222.7
(-3)
A 		305.7
(-1.2)
A 		264.6
(-2.1)
P 		283
B 314.9
(-3)
P 		327.3
(-0.5)
A 		344.5
(3)
P 		320.6
(-1.8)
P 		30
C 349.9
(2.1)
P 		252.4
(-3)
A 		313.6
(0.2)
A 		367.1
(3)
P 		115
avr 390.3
(3) 		267.5
(-3) 		321.3
(-0.4) 		317.4
(-0.6) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 272
1 2 3 4 5 6 range
A 234.6
(0.8)
M 		283.4
(3)
P 		151.8
(-3)
A 		275.1
(2.6)
A 		185.4
(-1.5)
A 		281.8
(2.9)
P 		132
B 297.7
(2.9)
P 		299.8
(3)
P 		196.3
(-1.7)
P 		168.9
(-3)
A 		285.5
(2.3)
P 		237.5
(0.1)
P 		131
C 423.8
(3)
P 		297.5
(0.2)
P 		218.4
(-1.6)
A 		155.6
(-3)
A 		306.6
(0.4)
M 		347.6
(1.3)
P 		268
avr 318.7
(3) 		293.6
(1.8) 		188.8
(-3) 		199.9
(-2.5) 		259.2
(0.3) 		289
(1.6) 		130
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 146
1 2 3 4 range
A 184.2
(3)
P 		48.3
(-2.6)
A 		79.9
(-1.3)
A 		38.7
(-3)
P 		146
B 100.4
(3)
P 		80.8
(-0.7)
A 		79.8
(-0.9)
P 		68.3
(-3)
P 		32
C 115.8
(3)
P 		72.6
(-1.8)
A 		109.3
(2.3)
A 		61.9
(-3)
P 		54
avr 133.5
(3) 		67.2
(-2.2) 		89.7
(-0.4) 		56.3
(-3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 43.2
(-2.1)
M 		100.8
(3)
P 		33.1
(-3)
A 		69.8
(0.3)
A 		55.9
(-1)
A 		68.6
(0.1)
P 		68
B 103.6
(2)
P 		99.8
(1.6)
P 		47.7
(-2.7)
P 		44.7
(-3)
A 		116
(3)
P 		88.6
(0.7)
P 		71
C 157.1
(3)
P 		90.8
(-0.2)
P 		55.6
(-1.8)
A 		31.3
(-3)
A 		99.2
(0.2)
M 		116.4
(1.1)
P 		126
avr 101.3
(3) 		97.1
(2.5) 		45.5
(-3) 		48.6
(-2.7) 		90.4
(1.8) 		91.2
(1.9) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177726_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6477
Sequence Type Exemplar sequence
Representative Public ID K01G5.9 NCBI
Target Description K01G5.9 /REP_DB=WormBase Gene ID /WP=CE19950 /TR=Q9XTB8 /GB=CAB07249.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066976(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066976 NCBI Caenorhabditis elegans K01G5.9 (K01G5.9) mRNA, complete cds. 10/11 A 
SNAP00000005062 ENSEMBL cdna:SNAP chromosome:CEL140:III:10759865:10762683:1 9/11 None
K01G5.9.2 ENSEMBL cdna:known chromosome:CEL140:III:10759238:10762875:1 gene:K01G5.9 10/11 A 
K01G5.9.1 ENSEMBL cdna:known chromosome:CEL140:III:10759262:10762875:1 gene:K01G5.9 10/11 A 
K01G5.9.3 ENSEMBL cdna:known chromosome:CEL140:III:10759546:10762875:1 gene:K01G5.9 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000005072 4/11 Cross Hyb Matching Probes None
GENEFINDER00000005070 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10759639-10762503(+) 99.87 99.87
Public Domain and Genome References
Gene Title
Ensembl K01G5.9
Entrez Gene 176508 Entrez gene
SwissProt Q9XTB8 EMBL-EBI
WormBase CE33132 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16289_AT asparaginase 2 family protein at
ATH1-121501:255684_AT asparaginase 2 family protein at
CANINE:1605716_AT similar to Threonine aspartase 1 (Taspase 1) cfa
CANINE_2:CFAAFFX.9349.1.S1_AT similar to Threonine aspartase 1 (Taspase 1) cfa
DROSOPHILA_2:1633766_AT dm
DROSGENOME1:153532_AT dm
CHICKEN:GGA.14160.1.S1_S_AT taspase, threonine aspartase, 1 gga
CHICKEN:GGAAFFX.5720.1.S1_S_AT taspase, threonine aspartase, 1 gga
HG-U133_PLUS_2:233719_S_AT taspase, threonine aspartase, 1 hs
HG-U133B:233719_S_AT taspase, threonine aspartase, 1 hs
HG-U133_PLUS_2:219443_AT taspase, threonine aspartase, 1 hs
HG-U133A:219443_AT taspase, threonine aspartase, 1 hs
HG-U133A_2:219443_AT taspase, threonine aspartase, 1 hs
HU35KSUBB:RC_H19673_AT taspase, threonine aspartase, 1 hs
HU35KSUBC:RC_AA261819_AT taspase, threonine aspartase, 1 hs
U133_X3P:G8923201_3P_AT taspase, threonine aspartase, 1 hs
U133_X3P:HS.306525.0.S1_3P_S_AT taspase, threonine aspartase, 1 hs
U133_X3P:HS.306525.0.S1_3P_AT taspase, threonine aspartase, 1 hs
HG-U95B:47731_AT taspase, threonine aspartase, 1 hs
HG-U95B:48312_AT taspase, threonine aspartase, 1 hs
HG-U95C:57514_AT taspase, threonine aspartase, 1 hs
HG-U95C:57518_G_AT taspase, threonine aspartase, 1 hs
HG-U95D:79951_AT Chromosome 20 open reading frame 13 hs
MOE430A:1451998_AT RIKEN cDNA 4930485D02 gene mm
MOUSE430A_2:1451998_AT RIKEN cDNA 4930485D02 gene mm
MOUSE430_2:1451998_AT RIKEN cDNA 4930485D02 gene mm
MOE430A:1424810_AT RIKEN cDNA 4930485D02 gene mm
MOUSE430A_2:1424810_AT RIKEN cDNA 4930485D02 gene mm
MOUSE430_2:1424810_AT RIKEN cDNA 4930485D02 gene mm
MG-U74BV2:113239_AT RIKEN cDNA 4930485D02 gene, mRNA (cDNA clone MGC:25800 IMAGE:4036044) mm
MG-U74CV2:130132_AT RIKEN cDNA 4930485D02 gene, mRNA (cDNA clone MGC:25800 IMAGE:4036044) mm
MU19KSUBA:TC21470_AT RIKEN cDNA 4930485D02 gene, mRNA (cDNA clone MGC:25800 IMAGE:4036044) mm
RG-U34B:RC_AA851187_AT similar to 4930485D02Rik protein (predicted) rn
RAE230B:1382428_AT similar to 4930485D02Rik protein (predicted) rn
RAT230_2:1382428_AT similar to 4930485D02Rik protein (predicted) rn
RG-U34C:RC_AI175451_AT Similar to 4930485D02Rik protein (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6516 glycoprotein catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4067 asparaginase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07249 Hypothetical protein K01G5.9 [Caenorhabditis elegans] emb|CAA16341.2| Hypothetical protein K01G5.9 [Caenorhabditis elegans] ref|NP_499377.2| K01G5.9 [Caenorhabditis elegans] 0.0
blast CAB07249 Hypothetical protein K01G5.9 [Caenorhabditis elegans] emb|CAA16341.2| Hypothetical protein K01G5.9 [Caenorhabditis elegans] ref|NP_499377.2| K01G5.9 [Caenorhabditis elegans] 1.0E-154
blast CAE71403 Hypothetical protein CBG18312 [Caenorhabditis briggsae] 1.0E-121
blast CAE71403 Hypothetical protein CBG18312 [Caenorhabditis briggsae] 1.0E-103
Protein Domains
Database ID Description E-Value
Pfam IPR000246 EMBL-EBI Peptidase T2, asparaginase 2 7.7E-4
Pfam IPR000246 EMBL-EBI Peptidase T2, asparaginase 2 5.5E-5
Sequence
Target Sequence 
>C. ELEGANS:177726_AT
ggattcggctcacatttgactatcgatcaagaagttgaatgtgaagcatcatatatgtca
tccaaaaatttaagtttcggcgcggtcggcgcgatttccaacgttttccatccgtcccga
gtggcacgtcaccttgcacatagcaattggtggaagcagcgacgtcttcttcatcccctt
attcttgttggtcgtggagcagagaaatatgctgttaaaaatgtaaaatgccaatttggt
ttgcaaaaaaaaaatcgattttcaaaagtttttccccctgaattctatcaaaatttcgta
tttttccaattttcgccacgaaaaatgtcttttttgcagacaatgaactcagaatccggt
acttcaagcggtggaattgtgctaaaacactcgggacggcttggtcacagctgtgtatat
ggaagtggaacatggtctgaacgaaggcaatacgaagaaccatttgatcaagtcagtgaa
cgaaccatctcgatttgttcgacaggacacggtgaatcacttgtgaaagccgatttctgc
aggggtatcgccacgagagtgctagac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATTCGGCTCACATTTGACTATCG 80 509 160 Antisense
GCACGTCACCTTGCACATAGCAATT 588 315 283 Antisense
GTGGAAGCAGCGACGTCTTCTTCAT 519 491 309 Antisense
CCCCTTATTCTTGTTGGTCGTGGAG 474 259 334 Antisense
TCGTATTTTTCCAATTTTCGCCACG 272 605 455 Antisense
GAACTCAGAATCCGGTACTTCAAGC 693 343 504 Antisense
GTGCTAAAACACTCGGGACGGCTTG 60 367 538 Antisense
GACGGCTTGGTCACAGCTGTGTATA 223 377 554 Antisense
GAACCATCTCGATTTGTTCGACAGG 19 347 641 Antisense
GAAAGCCGATTTCTGCAGGGGTATC 571 359 684 Antisense
GGGTATCGCCACGAGAGTGCTAGAC 504 497 702 Antisense
Sequence Source Affymetrix Proprietary Database