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[177723_at]

Description F54F7.7 /REP_DB=WormBase Gene ID /WP=CE03422 /TR=Q20794 /GB=CAA91760.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 90      4grp_total > 32      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 113.1
(-0.2)
A 		82.4
(-3)
A 		148
(3)
A 		89.7
(-2.3)
P 		66
B 63.2
(-3)
A 		139.6
(3)
A 		92.1
(-0.7)
A 		109.6
(0.6)
A 		76
C 82.1
(-3)
A 		98.5
(-1.3)
A 		105.1
(-0.7)
M 		141
(3)
A 		59
avr 86.1
(-3) 		106.8
(1.3) 		115.1
(3) 		113.4
(2.6) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 86.7
(3)
M 		54.7
(-2.8)
A 		53.5
(-3)
A 		70
(-0)
A 		76.1
(1.1)
A 		57.9
(-2.2)
A 		33
B 60.2
(-1.2)
P 		91.3
(3)
A 		78.5
(1.3)
M 		88
(2.6)
A 		47
(-3)
A 		67.3
(-0.3)
A 		44
C 58.6
(-1.3)
A 		60.9
(-1.1)
A 		41
(-3)
A 		103.3
(3)
A 		78.9
(0.7)
A 		69.4
(-0.3)
A 		62
avr 68.5
(-0.8) 		69
(-0.7) 		57.7
(-3) 		87.1
(3) 		67.3
(-1) 		64.9
(-1.5) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 41.2
(3)
A 		17.9
(-2)
A 		38.7
(2.5)
A 		13.1
(-3)
P 		28
B 20.2
(-3)
A 		34.5
(3)
A 		21.3
(-2.5)
A 		23.3
(-1.7)
A 		14
C 27.2
(-1.4)
A 		28.3
(-0.9)
A 		36.6
(3)
M 		23.8
(-3)
A 		13
avr 29.5
(1.7) 		26.9
(0.4) 		32.2
(3) 		20.1
(-3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 16
(-0.7)
M 		19.4
(1.1)
A 		11.6
(-3)
A 		17.7
(0.2)
A 		23
(3)
A 		14.1
(-1.7)
A 		11
B 20.9
(-2)
P 		30.4
(3)
A 		19.1
(-3)
M 		23.3
(-0.8)
A 		19.1
(-3)
A 		25.1
(0.2)
A 		11
C 21.7
(1.5)
A 		18.6
(0.3)
A 		10.4
(-3)
A 		20.8
(1.1)
A 		25.5
(3)
A 		23.2
(2.1)
A 		15
avr 19.5
(0.8) 		22.8
(3) 		13.7
(-3) 		20.6
(1.5) 		22.5
(2.8) 		20.8
(1.7) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177723_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16752
Sequence Type Exemplar sequence
Representative Public ID F54F7.7 NCBI
Target Description F54F7.7 /REP_DB=WormBase Gene ID /WP=CE03422 /TR=Q20794 /GB=CAA91760.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077580(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077580 NCBI Caenorhabditis elegans F54F7.7 (F54F7.7) mRNA, complete cds. 11/11 None
SNAP00000009563 ENSEMBL cdna:SNAP chromosome:CEL140:X:11867955:11869281:-1 10/11 None
F54F7.7 ENSEMBL cdna:known chromosome:CEL140:X:11867892:11869281:-1 gene:F54F7.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000009567 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11867863-11869281(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54F7.7
Entrez Gene 186255 Entrez gene
SwissProt Q20794 EMBL-EBI
WormBase CE35555 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91760 Hypothetical protein F54F7.7 [Caenorhabditis elegans] ref|NP_509981.2| F54F7.7 [Caenorhabditis elegans] 1.0E-178
blast CAA91760 Hypothetical protein F54F7.7 [Caenorhabditis elegans] ref|NP_509981.2| F54F7.7 [Caenorhabditis elegans] 1.0E-148
blast CAA93538 Hypothetical protein W06D11.4 [Caenorhabditis elegans] ref|NP_510056.1| W06D11.4 [Caenorhabditis elegans] 2.0E-91
blast CAA93538 Hypothetical protein W06D11.4 [Caenorhabditis elegans] ref|NP_510056.1| W06D11.4 [Caenorhabditis elegans] 1.0E-77
blast CAA90610 Hypothetical protein F55G7.2 [Caenorhabditis elegans] ref|NP_509997.1| F55G7.2 [Caenorhabditis elegans] 1.0E-38
blast CAE60379 Hypothetical protein CBG03980 [Caenorhabditis briggsae] 3.0E-31
Sequence
Target Sequence 
>C. ELEGANS:177723_AT
gcgtggagatcatgcccaacttggcacaaatggcaagagccaacgagcagttcagcaaat
cgcttctgcgccacttcggcaaaactgtgaatccaactcgacttgttcacggatcgttca
ccagtcccgatatcgttcacgagatccagaacgaagctacggtcatctgtgtgaacaaca
tcaagttttcggcggaactcaagttggagctgaaaatgatcttgtcaaaatgtgctgacg
gaacgcggatcatttcatcggagtcgattgctccgcgccctcgttctcaaaactcgacat
ctagtcgtgtcgatgagttcgtgcgcatctccactgagcgccctcttcttctggtggaaa
gcaatacgtcttggacaggcaacacggttcctttctacctcaccgaaatcaaacacaatc
aggtactgatcgtcgtcgtccatccatctccacagcttctatgtcaccaagacgtcctcg
ttctcgggtcaatttctacttttccggttaattttttattcaagatctccccttcatccc
ctatagatctcacgactcctttgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGTGGAGATCATGCCCAACTTGGC 101 293 536 Antisense
TTCTGCGCCACTTCGGCAAAACTGT 119 693 599 Antisense
AACTCGACTTGTTCACGGATCGTTC 453 141 630 Antisense
CCAGTCCCGATATCGTTCACGAGAT 312 271 656 Antisense
GAACGAAGCTACGGTCATCTGTGTG 617 347 684 Antisense
TCATCGGAGTCGATTGCTCCGCGCC 558 619 790 Antisense
GCCCTCTTCTTCTGGTGGAAAGCAA 689 285 875 Antisense
TTGGACAGGCAACACGGTTCCTTTC 244 655 906 Antisense
TTCCTTTCTACCTCACCGAAATCAA 347 685 923 Antisense
GCTTCTATGTCACCAAGACGTCCTC 633 303 990 Antisense
CTATAGATCTCACGACTCCTTTGTT 227 223 1076 Antisense
Sequence Source Affymetrix Proprietary Database