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[177714_at]

Description ZK896.1 /REP_DB=WormBase Gene ID /WP=CE15449 /TR=O02341 /GB=CAB05322.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 33      4grp_total > 53      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 140.5
(3)
A 		116
(1.8)
A 		104.4
(1.3)
A 		15.1
(-3)
A 		125
B 150.9
(3)
A 		121.9
(1.6)
A 		74.5
(-0.8)
A 		29
(-3)
A 		122
C 151.8
(3)
A 		119.7
(-0.1)
A 		90.6
(-3)
A 		105.4
(-1.5)
A 		61
avr 147.7
(3) 		119.2
(1.3) 		89.8
(-0.5) 		49.8
(-3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 91.4
(2.6)
A 		71.1
(0.4)
A 		94.7
(3)
A 		55.3
(-1.3)
A 		80.5
(1.4)
A 		40
(-3)
A 		55
B 98.7
(3)
A 		73.9
(1.3)
A 		90.8
(2.4)
A 		63.6
(0.5)
A 		74.9
(1.3)
A 		13.2
(-3)
A 		86
C 100.4
(3)
A 		82.2
(1.3)
A 		89.8
(2)
A 		62.5
(-0.5)
A 		45.8
(-2.1)
A 		36.1
(-3)
A 		64
avr 96.8
(3) 		75.7
(1.1) 		91.8
(2.6) 		60.5
(-0.3) 		67.1
(0.3) 		29.8
(-3) 		67
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 51.1
(3)
A 		25.2
(-0.2)
A 		27.3
(0.1)
A 		2.2
(-3)
A 		49
B 48.1
(3)
A 		30.1
(0.4)
A 		17.3
(-1.4)
A 		6.2
(-3)
A 		42
C 50.2
(3)
A 		34.4
(0.1)
A 		31.6
(-0.4)
A 		17.8
(-3)
A 		32
avr 49.8
(3) 		29.9
(0.1) 		25.4
(-0.6) 		8.7
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 16.8
(-0.3)
A 		25.3
(3)
A 		20.6
(1.2)
A 		14
(-1.3)
A 		24.3
(2.6)
A 		9.7
(-3)
A 		16
B 34.4
(3)
A 		24.6
(1)
A 		22
(0.5)
A 		16.9
(-0.6)
A 		30.4
(2.2)
A 		4.9
(-3)
A 		30
C 37.2
(3)
A 		25.1
(0.1)
A 		22.9
(-0.4)
A 		12.6
(-2.9)
A 		14.8
(-2.4)
A 		12.1
(-3)
A 		25
avr 29.5
(3) 		25
(1.7) 		21.8
(0.8) 		14.5
(-1.4) 		23.2
(1.2) 		8.9
(-3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177714_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9952
Sequence Type Exemplar sequence
Representative Public ID ZK896.1 NCBI
Target Description ZK896.1 /REP_DB=WormBase Gene ID /WP=CE15449 /TR=O02341 /GB=CAB05322.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070051(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070051 NCBI Caenorhabditis elegans ZK896.1 (ZK896.1) mRNA, complete cds. 11/11 None
GENEFINDER00000024394 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12872799:12875315:-1 11/11 None
ZK896.1 ENSEMBL cdna:known chromosome:CEL140:IV:12872799:12875315:-1 gene:ZK896.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000024386 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12872804-12875321(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK896.1
Entrez Gene 191449 Entrez gene
SwissProt O02341 EMBL-EBI
WormBase CE15449 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05322 Hypothetical protein ZK896.1 [Caenorhabditis elegans] ref|NP_502452.1| ZK896.1 [Caenorhabditis elegans] 0.0
blast CAB05319 Hypothetical protein ZK896.4 [Caenorhabditis elegans] ref|NP_502454.1| ZK896.4 [Caenorhabditis elegans] 1.0E-107
blast CAB05320 Hypothetical protein ZK896.5 [Caenorhabditis elegans] ref|NP_502453.1| ZK896.5 [Caenorhabditis elegans] 1.0E-104
blast CAB05319 Hypothetical protein ZK896.4 [Caenorhabditis elegans] ref|NP_502454.1| ZK896.4 [Caenorhabditis elegans] 4.0E-77
blast CAB05320 Hypothetical protein ZK896.5 [Caenorhabditis elegans] ref|NP_502453.1| ZK896.5 [Caenorhabditis elegans] 5.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 1.9E-26
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 2.3E-34
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 7.9E-6
Sequence
Target Sequence 
>C. ELEGANS:177714_AT
gaactgctgcagttcttcttttcaatagtttatcctttgtgaatcaactttcaatgtcag
ataacaacagcacgctctcggtatatacaaacagtattagtgatgacaataagctttccg
attactcatactcaaactcccaaaccaacattcctcaaaaatttgttggaaactacacga
tatttgttctagacaagggtcaagctaatctcagcttgaaaacatatacaccgaataatg
cgtggtggtcttatgttttttacggacagcgaggattctttgcatcacctaactttggaa
caagtgattctgatcaaaatctttgtgacgcagtatctatcaatggcaagcatccagcga
agatcacctacactgttgatagatctgcaattgttggtcctgctgttctcactgtttcca
ttggaggcaatataactatatatgacgtcaccaaccttcctggaccggaccccatctctg
cgataggaatgtctattgccgtcaattatgaatcaaatggtgttatttcgacaggggcat
ttgtatcctttgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACTGCTGCAGTTCTTCTTTTCAA 129 349 617 Antisense
AACAGCACGCTCTCGGTATATACAA 250 137 682 Antisense
AAGCTTTCCGATTACTCATACTCAA 83 151 727 Antisense
AATGCGTGGTGGTCTTATGTTTTTT 383 165 853 Antisense
GACAGCGAGGATTCTTTGCATCACC 594 367 881 Antisense
GACGCAGTATCTATCAATGGCAAGC 391 379 943 Antisense
GGCAAGCATCCAGCGAAGATCACCT 94 533 961 Antisense
TGCAATTGTTGGTCCTGCTGTTCTC 264 577 1002 Antisense
TATATATGACGTCACCAACCTTCCT 135 661 1053 Antisense
GACCCCATCTCTGCGATAGGAATGT 105 381 1084 Antisense
GACAGGGGCATTTGTATCCTTTGAA 193 369 1146 Antisense
Sequence Source Affymetrix Proprietary Database