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[177711_at]

Description C35D6.5 /REP_DB=WormBase Gene ID /WP=CE15684 /TR=O45290 /GB=CAB05148.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 81      4grp_total > 42      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 50.3
(-0.2)
A 		65.2
(3)
A 		37.5
(-3)
A 		49.2
(-0.5)
A 		28
B 57.3
(0.1)
A 		53
(-1.3)
A 		65.8
(3)
A 		48.1
(-3)
A 		18
C 41.5
(-3)
A 		86.2
(3)
A 		62.3
(-0.2)
A 		62.2
(-0.2)
P 		45
avr 49.7
(-3) 		68.1
(3) 		55.2
(-1.2) 		53.2
(-1.9) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 50
(-3)
A 		65.2
(-0.3)
A 		73
(1.1)
A 		83.6
(3)
A 		81.9
(2.7)
P 		78.9
(2.2)
P 		34
B 65.2
(0.4)
M 		78.1
(3)
A 		48.1
(-3)
P 		67
(0.8)
A 		52.4
(-2.1)
M 		71.6
(1.7)
P 		30
C 52.4
(-3)
A 		55.7
(-2.6)
A 		61.8
(-1.9)
A 		102.2
(3)
P 		94.4
(2.1)
A 		58.4
(-2.3)
A 		50
avr 55.9
(-3) 		66.3
(-0.8) 		61
(-1.9) 		84.3
(3) 		76.2
(1.3) 		69.6
(-0.1) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 18.3
(3)
A 		14.2
(0.8)
A 		9.8
(-1.6)
A 		7.2
(-3)
A 		11
B 18.3
(3)
A 		13.1
(-0.9)
A 		15.3
(0.8)
A 		10.3
(-3)
A 		8
C 13.7
(-1.7)
A 		24.8
(3)
A 		21.7
(1.7)
A 		10.5
(-3)
P 		14
avr 16.8
(2.6) 		17.4
(3) 		15.6
(1.7) 		9.3
(-3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 9.2
(-3)
A 		23.2
(2.4)
A 		15.9
(-0.4)
A 		21.2
(1.6)
A 		24.7
(3)
P 		19.2
(0.9)
P 		16
B 22.7
(1.4)
M 		26
(2.7)
A 		11.7
(-3)
P 		17.7
(-0.6)
A 		21.3
(0.8)
M 		26.7
(3)
P 		15
C 19.4
(-1.5)
A 		17
(-2.5)
A 		15.7
(-3)
A 		20.5
(-1.1)
P 		30.5
(3)
A 		19.5
(-1.5)
A 		15
avr 17.1
(-1.5) 		22.1
(1.2) 		14.4
(-3) 		19.8
(-0.1) 		25.5
(3) 		21.8
(1) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177711_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10226
Sequence Type Exemplar sequence
Representative Public ID C35D6.5 NCBI
Target Description C35D6.5 /REP_DB=WormBase Gene ID /WP=CE15684 /TR=O45290 /GB=CAB05148.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070549(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070549 NCBI Caenorhabditis elegans C35D6.5 (C35D6.5) mRNA, complete cds. 11/11 None
GENEFINDER00000023359 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16366957:16368904:1 9/11 None
C35D6.5 ENSEMBL cdna:known chromosome:CEL140:IV:16364276:16368904:1 gene:C35D6.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000023355 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16364281-16368910(+) 89.71 100.0
Public Domain and Genome References
Gene Title
Ensembl C35D6.5
Entrez Gene 183236 Entrez gene
SwissProt O45290 EMBL-EBI
WormBase CE15684 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05148 Hypothetical protein C35D6.5 [Caenorhabditis elegans] ref|NP_502950.1| C35D6.5 [Caenorhabditis elegans] 1.0E-159
blast CAB05148 Hypothetical protein C35D6.5 [Caenorhabditis elegans] ref|NP_502950.1| C35D6.5 [Caenorhabditis elegans] 4.0E-92
blast AAB70362 Hypothetical protein K06H6.2 [Caenorhabditis elegans] ref|NP_503254.1| K06H6.2 [Caenorhabditis elegans] 2.0E-91
blast AAB70362 Hypothetical protein K06H6.2 [Caenorhabditis elegans] ref|NP_503254.1| K06H6.2 [Caenorhabditis elegans] 2.0E-80
blast AAK85471 Hypothetical protein F28A10.5 [Caenorhabditis elegans] ref|NP_493833.1| F28A10.5 [Caenorhabditis elegans] 4.0E-73
blast CAB54371 Hypothetical protein Y105C5B.23 [Caenorhabditis elegans] ref|NP_502912.1| Y105C5B.23 [Caenorhabditis elegans] 2.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR007744 EMBL-EBI Protein of unknown function DUF672 1.0E-126
Pfam IPR007744 EMBL-EBI Protein of unknown function DUF672 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000023359 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:177711_AT
ggcacagatttttatttcctgacacaaaatgtactacaacacaaactcgggctcattgag
catattgatacttttcatattgatattaatttcaatatcaaactatttgagtttcatttg
ctgtctctcggtctgaataatcaaattggctatgacagccatatttatgaagtcaccgga
aaacaatgcaaaattctcggagcagataaggatccccaaaggcctaacaccaaagcttcc
tacgagaaaatgaacggacaacttttttcgggaaaaattcccgacgagctcacaattccc
tcaatgctccagaaatccgggagaaatgaggtggagctgctgaaaattgatatcgaagga
ggagagttcaccgggttggagccgctgattagtgaatattttgtgtgtcagattttcatc
gaagtgcacggaaaacccgctgagcacctgaaaatgctccgaataatgtcgaaatacggt
ttccgtttattcaacattgatgagaacccgtattgtgagaattgctgtgaatatagtctg
atcaacgagctctgtatggctcaattcggagttgtaccgctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCACAGATTTTTATTTCCTGACAC 333 535 217 Antisense
GTACTACAACACAAACTCGGGCTCA 409 459 247 Antisense
TTGAGTTTCATTTGCTGTCTCTCGG 610 705 323 Antisense
GCTGTCTCTCGGTCTGAATAATCAA 143 295 336 Antisense
GATAAGGATCCCCAAAGGCCTAACA 118 421 421 Antisense
CCTAACACCAAAGCTTCCTACGAGA 630 265 439 Antisense
AAAAATTCCCGACGAGCTCACAATT 484 127 489 Antisense
CCCTCAATGCTCCAGAAATCCGGGA 570 259 514 Antisense
GTTGGAGCCGCTGATTAGTGAATAT 563 435 591 Antisense
GTGCACGGAAAACCCGCTGAGCACC 59 481 640 Antisense
GGCTCAATTCGGAGTTGTACCGCTG 376 537 774 Antisense
Sequence Source Affymetrix Proprietary Database