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[177701_s_at]

Description ZK896.2 /REP_DB=WormBase Gene ID /WP=CE15451 /TR=O02342 /GB=CAB05323.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 46      4grp_highest_8_8 > 33      4grp_high_8_8 > 58      6grp_highest_nonorm > 72      6grp_total > 45      4grp_total > 61      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 431
1 2 3 4 range
A 647.2
(3)
P 		554.6
(-0.4)
P 		566.9
(0)
P 		485.2
(-3)
P 		162
B 864.1
(3)
P 		725.5
(1.1)
P 		834.2
(2.6)
P 		433.6
(-3)
P 		431
C 757.8
(3)
P 		625.9
(0.4)
P 		723.1
(2.3)
P 		451.5
(-3)
P 		306
avr 756.4
(3) 		635.3
(0.6) 		708.1
(2) 		456.8
(-3) 		300

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 334
1 2 3 4 5 6 range
A 408.4
(-1.1)
P 		427.2
(-0.7)
P 		609.2
(3)
P 		425.6
(-0.8)
P 		316
(-3)
P 		381.9
(-1.7)
P 		293
B 538.6
(3)
P 		432.3
(0.2)
P 		400.1
(-0.7)
P 		314.6
(-3)
P 		334.6
(-2.5)
P 		468.7
(1.1)
P 		224
C 425.3
(1.8)
P 		454
(2.8)
P 		340
(-0.9)
P 		334.6
(-1.1)
P 		275.4
(-3)
P 		461.3
(3)
P 		186
avr 457.4
(3) 		437.8
(2.2) 		449.8
(2.7) 		358.3
(-1) 		308.7
(-3) 		437.3
(2.2) 		149
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 205
1 2 3 4 range
A 235.6
(3)
P 		120.4
(-1.2)
P 		148.2
(-0.2)
P 		71
(-3)
P 		165
B 275.5
(3)
P 		179.2
(-0.2)
P 		193.3
(0.3)
P 		92.4
(-3)
P 		183
C 250.8
(3)
P 		179.9
(0.5)
P 		252
(3)
P 		76.1
(-3)
P 		176
avr 254
(3) 		159.8
(-0.2) 		197.8
(1.1) 		79.8
(-3) 		174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 120
1 2 3 4 5 6 range
A 75.3
(-3)
P 		151.9
(3)
P 		132.7
(1.5)
P 		107.9
(-0.4)
P 		95.3
(-1.4)
P 		93
(-1.6)
P 		77
B 187.4
(3)
P 		143.9
(0.5)
P 		97.2
(-2.2)
P 		83.3
(-3)
P 		135.9
(0)
P 		174.8
(2.3)
P 		104
C 157.6
(3)
P 		138.5
(1.7)
P 		86.5
(-1.7)
P 		67.3
(-3)
P 		89.1
(-1.6)
P 		154.4
(2.8)
P 		90
avr 140.1
(2.5) 		144.8
(3) 		105.5
(-1) 		86.2
(-3) 		106.8
(-0.9) 		140.7
(2.6) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177701_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8417
Sequence Type Exemplar sequence
Representative Public ID ZK896.2 NCBI
Target Description ZK896.2 /REP_DB=WormBase Gene ID /WP=CE15451 /TR=O02342 /GB=CAB05323.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070056(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070056 NCBI Caenorhabditis elegans K08D8.6 (K08D8.6) mRNA, complete cds. 11/11 None
GENEFINDER00000024397 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12887262:12888857:-1 11/11 None
K08D8.6 ENSEMBL cdna:known chromosome:CEL140:IV:12887110:12890478:-1 gene:K08D8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12886755-12888711(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K08D8.6
Entrez Gene 178241 Entrez gene
SwissProt O01636 EMBL-EBI
WormBase CE37842 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA97438 Hypothetical protein K08D8.6 [Caenorhabditis elegans] emb|CAB08161.2| Hypothetical protein K08D8.6 [Caenorhabditis elegans] emb|CAB05328.2| Hypothetical protein K08D8.6 [Caenorhabditis elegans] ref|NP_502457.2| K08D8.6 [Caenorhabditis elegans] 0.0
blast T28080 hypothetical protein ZK896.2 - Caenorhabditis elegans 1.0E-170
blast CAB05320 Hypothetical protein ZK896.5 [Caenorhabditis elegans] ref|NP_502453.1| ZK896.5 [Caenorhabditis elegans] 1.0E-142
blast CAB05320 Hypothetical protein ZK896.5 [Caenorhabditis elegans] ref|NP_502453.1| ZK896.5 [Caenorhabditis elegans] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 1.2E-43
Sequence
Target Sequence 
>C. ELEGANS:177701_S_AT
tcctggaggaattctatacactattggagactccatatcggtgaaatatgactccaatgg
tgctgtatcaactggaccatttgtctcttttggatttgaccggtacagttcggcggcttg
taatggaattttcatcacaatcatcatggctatctggatgggactttccct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTGGAGGAATTCTATACACTATT 52 591 750 Antisense
TTCTATACACTATTGGAGACTCCAT 636 689 761 Antisense
TTGGAGACTCCATATCGGTGAAATA 639 685 773 Antisense
GGTGAAATATGACTCCAATGGTGCT 578 497 789 Antisense
GACTCCAATGGTGCTGTATCAACTG 581 373 799 Antisense
TCAACTGGACCATTTGTCTCTTTTG 443 631 817 Antisense
GTCTCTTTTGGATTTGACCGGTACA 482 469 832 Antisense
TTTGACCGGTACAGTTCGGCGGCTT 710 657 844 Antisense
TCGGCGGCTTGTAATGGAATTTTCA 167 605 859 Antisense
ATCACAATCATCATGGCTATCTGGA 635 27 883 Antisense
TGGCTATCTGGATGGGACTTTCCCT 96 545 896 Antisense
Sequence Source Affymetrix Proprietary Database