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[177700_at]

Description ZK896.2 /REP_DB=WormBase Gene ID /WP=CE15451 /TR=O02342 /GB=CAB05323.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 03      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 51      6grp_total > 24      4grp_total > 60      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 648
1 2 3 4 range
A 860
(2)
P 		946.8
(3)
P 		842.4
(1.8)
P 		408.2
(-3)
P 		539
B 914.5
(3)
P 		773.3
(1.3)
P 		868
(2.5)
P 		405.1
(-3)
P 		509
C 878.6
(1.3)
P 		745.2
(0)
P 		1053
(3)
P 		436.4
(-3)
P 		617
avr 884.4
(2.6) 		821.8
(1.8) 		921.1
(3) 		416.6
(-3) 		505

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 363
1 2 3 4 5 6 range
A 505.5
(-0.3)
P 		629.5
(3)
P 		623.6
(2.8)
P 		460.6
(-1.4)
P 		401
(-3)
P 		406.8
(-2.8)
P 		229
B 702.9
(3)
P 		576.5
(0.3)
P 		474
(-1.8)
P 		469.7
(-1.9)
P 		418.6
(-3)
P 		418.9
(-3)
P 		284
C 612.1
(3)
P 		522.7
(1)
P 		539.7
(1.4)
P 		410.4
(-1.5)
P 		340.4
(-3)
P 		351.2
(-2.8)
P 		272
avr 606.8
(3) 		576.2
(2.2) 		545.8
(1.3) 		446.9
(-1.4) 		386.7
(-3) 		392.3
(-2.8) 		220
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 307
1 2 3 4 range
A 313.1
(3)
P 		205.5
(0.5)
P 		220.2
(0.8)
P 		59.8
(-3)
P 		253
B 291.6
(3)
P 		191
(0.1)
P 		201.1
(0.4)
P 		86.3
(-3)
P 		205
C 290.8
(1.4)
P 		214.2
(-0.1)
P 		367
(3)
P 		73.6
(-3)
P 		293
avr 298.5
(3) 		203.6
(0.5) 		262.8
(2) 		73.2
(-3) 		225

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 93.2
(-3)
P 		223.9
(3)
P 		135.8
(-1)
P 		116.8
(-1.9)
P 		121
(-1.7)
P 		99
(-2.7)
P 		131
B 244.6
(3)
P 		191.9
(0.6)
P 		115.1
(-3)
P 		124.4
(-2.6)
P 		170.1
(-0.5)
P 		156.2
(-1.1)
P 		130
C 226.9
(3)
P 		159.5
(0.2)
P 		137.4
(-0.7)
P 		82.5
(-3)
P 		110.2
(-1.8)
P 		117.6
(-1.5)
P 		144
avr 188.2
(2.7) 		191.8
(3) 		129.4
(-1.5) 		107.9
(-3) 		133.8
(-1.1) 		124.3
(-1.8) 		84
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177700_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8417
Sequence Type Exemplar sequence
Representative Public ID ZK896.2 NCBI
Target Description ZK896.2 /REP_DB=WormBase Gene ID /WP=CE15451 /TR=O02342 /GB=CAB05323.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070056(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070056 NCBI Caenorhabditis elegans K08D8.6 (K08D8.6) mRNA, complete cds. 11/11 None
GENEFINDER00000024397 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12887262:12888857:-1 11/11 None
K08D8.6 ENSEMBL cdna:known chromosome:CEL140:IV:12887110:12890478:-1 gene:K08D8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12886755-12888711(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K08D8.6
Entrez Gene 178241 Entrez gene
SwissProt O01636 EMBL-EBI
WormBase CE37842 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA97438 Hypothetical protein K08D8.6 [Caenorhabditis elegans] emb|CAB08161.2| Hypothetical protein K08D8.6 [Caenorhabditis elegans] emb|CAB05328.2| Hypothetical protein K08D8.6 [Caenorhabditis elegans] ref|NP_502457.2| K08D8.6 [Caenorhabditis elegans] 0.0
blast T28080 hypothetical protein ZK896.2 - Caenorhabditis elegans 1.0E-170
blast CAB05320 Hypothetical protein ZK896.5 [Caenorhabditis elegans] ref|NP_502453.1| ZK896.5 [Caenorhabditis elegans] 1.0E-142
blast CAB05320 Hypothetical protein ZK896.5 [Caenorhabditis elegans] ref|NP_502453.1| ZK896.5 [Caenorhabditis elegans] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 1.2E-43
Sequence
Target Sequence 
>C. ELEGANS:177700_AT
gactacacatcatcaatctccaaaaccaacactccacagaagtttagtggaaagttcaca
accttcgttctcaacaaagatcaagctactttaagaatgtcgtcagccgagaaaaccgct
gaatggaatacggcatttgatagtcgtcgaggatttttcacctctggcaattatggattg
aacagttcgattcaaaatattgccgatcaaattactgcaagcaaactgacgaatatcagt
tttactgttgatcggactgcaattattggaggtgcatctcttacagttaagattacttct
gaacagaaaactgtgctgaacaatgtgtacactacttccaactttcctggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTACACATCATCAATCTCCAAAA 214 373 406 Antisense
TCCAAAACCAACACTCCACAGAAGT 63 587 424 Antisense
TGGAAAGTTCACAACCTTCGTTCTC 358 547 453 Antisense
TCACAACCTTCGTTCTCAACAAAGA 618 627 461 Antisense
GAATGTCGTCAGCCGAGAAAACCGC 676 329 500 Antisense
GGAATACGGCATTTGATAGTCGTCG 537 533 530 Antisense
GATAGTCGTCGAGGATTTTTCACCT 193 423 544 Antisense
GATTTTTCACCTCTGGCAATTATGG 159 429 557 Antisense
GTTTTACTGTTGATCGGACTGCAAT 223 447 644 Antisense
ATTGGAGGTGCATCTCTTACAGTTA 620 3 670 Antisense
TACACTACTTCCAACTTTCCTGGAG 202 639 733 Antisense
Sequence Source Affymetrix Proprietary Database