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[177699_at]

Description K04G2.6 /REP_DB=WormBase Gene ID /WP=CE06100 /TR=Q21225 /GB=CAB00043.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 34      4grp_highest_8_8 > 25      4grp_high_8_8 > 51      6grp_highest_nonorm > 73      6grp_total > 30      4grp_total > 60      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 232
1 2 3 4 range
A 550
(1.5)
P 		517.7
(-1.2)
P 		567.9
(3)
P 		496.5
(-3)
P 		71
B 607.9
(3)
P 		512.7
(0.2)
P 		552.7
(1.4)
P 		405.2
(-3)
P 		203
C 492
(-0)
P 		608.8
(3)
P 		540.6
(1.2)
P 		376.5
(-3)
P 		232
avr 550
(2.8) 		546.4
(2.7) 		553.7
(3) 		426.1
(-3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 262
1 2 3 4 5 6 range
A 623.5
(3)
P 		589.5
(1.9)
P 		441.2
(-3)
P 		614.2
(2.7)
P 		613.7
(2.7)
P 		544
(0.4)
P 		182
B 450.8
(-3)
P 		568.2
(3)
P 		463.5
(-2.4)
P 		537.8
(1.4)
P 		478.8
(-1.6)
P 		473
(-1.9)
P 		117
C 535.3
(-0.9)
P 		547
(-0.6)
P 		702.7
(3)
P 		526.9
(-1.1)
P 		443.2
(-3)
P 		538.3
(-0.8)
P 		260
avr 536.5
(-0.4) 		568.2
(3) 		535.8
(-0.5) 		559.6
(2.1) 		511.9
(-3) 		518.4
(-2.3) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 200.3
(3)
P 		112.4
(-1.1)
P 		148.5
(0.6)
P 		72.7
(-3)
P 		128
B 193.9
(3)
P 		126.6
(-0.8)
P 		128.1
(-0.7)
P 		86.3
(-3)
P 		108
C 162.9
(1.8)
P 		175
(2.4)
P 		188.4
(3)
P 		63.5
(-3)
P 		125
avr 185.7
(3) 		138
(0.4) 		155
(1.3) 		74.2
(-3) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 114.9
(-2)
P 		209.7
(3)
P 		96.1
(-3)
P 		155.8
(0.2)
P 		185.2
(1.7)
P 		132.5
(-1.1)
P 		114
B 156.9
(0.2)
P 		189.1
(2.6)
P 		112.6
(-3)
P 		142.5
(-0.8)
P 		194.5
(3)
P 		176.4
(1.7)
P 		82
C 198.4
(3)
P 		166.9
(1)
P 		178.8
(1.7)
P 		106
(-3)
P 		143.4
(-0.6)
P 		180.2
(1.8)
P 		92
avr 156.7
(-0.2) 		188.6
(3) 		129.2
(-3) 		134.8
(-2.4) 		174.4
(1.6) 		163
(0.4) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177699_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1505
Sequence Type Exemplar sequence
Representative Public ID K04G2.6 NCBI
Target Description K04G2.6 /REP_DB=WormBase Gene ID /WP=CE06100 /TR=Q21225 /GB=CAB00043.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059814(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059814 NCBI Caenorhabditis elegans K04G2.6 (K04G2.6) mRNA, complete cds. 11/11 None
SNAP00000009074 ENSEMBL cdna:SNAP chromosome:CEL140:I:8048926:8051246:1 11/11 None
GENEFINDER00000009098 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8048926:8051246:1 11/11 None
K04G2.6.1 ENSEMBL cdna:known chromosome:CEL140:I:8048923:8051398:1 gene:K04G2.6 11/11 None
K04G2.6.2 ENSEMBL cdna:known chromosome:CEL140:I:8048925:8051246:1 gene:K04G2.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8048856-8051177(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K04G2.6
Entrez Gene 172589 Entrez gene
SwissProt Q21225 EMBL-EBI
WormBase CE06100 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.21613.1.S1_AT similar to Vac14 homolog cfa
CANINE_2:CFAAFFX.30874.1.S1_S_AT similar to Vac14 homolog cfa
DROSOPHILA_2:1640366_AT dm
DROSGENOME1:141609_AT dm
CHICKEN:GGA.10501.2.S1_S_AT Vac14 homolog (S. cerevisiae) gga
CHICKEN:GGAAFFX.1647.1.S1_S_AT Vac14 homolog (S. cerevisiae) gga
CHICKEN:GGAAFFX.25494.1.S1_AT Vac14 homolog (S. cerevisiae) gga
CHICKEN:GGA.10501.1.S1_AT Similar to hypothetical protein FLJ10305 gga
CHICKEN:GGA.10501.2.A1_AT Similar to hypothetical protein FLJ10305 gga
HG-U95AV2:830_AT Vac14 homolog (S. cerevisiae) hs
HC-G110:830_AT Vac14 homolog (S. cerevisiae) hs
HU35KSUBA:HUM_ALU_AT Vac14 homolog (S. cerevisiae) hs
HU35KSUBB:HUM_ALU_AT Vac14 homolog (S. cerevisiae) hs
HU35KSUBC:HUM_ALU_AT Vac14 homolog (S. cerevisiae) hs
HU35KSUBD:HUM_ALU_AT Vac14 homolog (S. cerevisiae) hs
HUGENEFL:HUM_ALU_AT Vac14 homolog (S. cerevisiae) hs
HC-G110:HUM_ALU_AT Vac14 homolog (S. cerevisiae) hs
HU35KSUBB:RC_N23933_AT Vac14 homolog (S. cerevisiae) hs
HUGENEFL:U25801_AT Vac14 homolog (S. cerevisiae) hs
HG-U133A:218169_AT Vac14 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:218169_AT Vac14 homolog (S. cerevisiae) hs
HG-U133A_2:218169_AT Vac14 homolog (S. cerevisiae) hs
U133_X3P:G8922339_3P_X_AT Vac14 homolog (S. cerevisiae) hs
U133_X3P:G8922339_3P_AT Vac14 homolog (S. cerevisiae) hs
U133_X3P:HS.287365.0.S1_3P_AT Vac14 homolog (S. cerevisiae) hs
HG-U133A_2:216407_AT Vac14 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:216407_AT Vac14 homolog (S. cerevisiae) hs
HG-U133A:216407_AT Vac14 homolog (S. cerevisiae) hs
HG-U95C:64468_AT Vac14 homolog (S. cerevisiae) hs
HG-U95E:86038_AT Vac14 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:216501_AT Vac14 homolog (S. cerevisiae) hs
HG-U133A_2:216501_AT Vac14 homolog (S. cerevisiae) hs
HG-U133A:216501_AT Vac14 homolog (S. cerevisiae) hs
U133_X3P:HS2.376295.3.S1_3P_AT Vac14 homolog (S. cerevisiae) hs
U133_X3P:HS2.376295.1.S1_3P_A_AT Vac14 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:1564158_A_AT Vac14 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:1569276_AT Vac14 homolog (S. cerevisiae) hs
HG-U133_PLUS_2:244711_AT Vac14 homolog (S. cerevisiae) hs
HG-U133B:244711_AT Vac14 homolog (S. cerevisiae) hs
HG-U95E:67699_R_AT Vac14 homolog (S. cerevisiae) hs
U133_X3P:HS.299668.0.S1_3P_AT Vac14 homolog (S. cerevisiae) hs
MU11KSUBA:AA670618_G_AT Vac14 homolog (S. cerevisiae) mm
MU11KSUBA:AA670618_AT Vac14 homolog (S. cerevisiae) mm
MG-U74AV2:96023_AT Vac14 homolog (S. cerevisiae) mm
MG-U74CV2:169226_R_AT Vac14 homolog (S. cerevisiae) mm
MOE430A:1416231_AT Vac14 homolog (S. cerevisiae) mm
MOUSE430A_2:1416231_AT Vac14 homolog (S. cerevisiae) mm
MOUSE430_2:1416231_AT Vac14 homolog (S. cerevisiae) mm
MU19KSUBB:TC29980_AT Vac14 homolog (S. cerevisiae) (Vac14), mRNA mm
MU19KSUBC:TC36951_AT Vac14 homolog (S. cerevisiae) (Vac14), mRNA mm
MU19KSUBC:TC36951_G_AT Vac14 homolog (S. cerevisiae) (Vac14), mRNA mm
RG-U34B:RC_AI008813_AT vacuole 14 protein (S. cerevisiae) rn
RAE230A:1389041_AT vacuole 14 protein (S. cerevisiae) rn
RAT230_2:1389041_AT vacuole 14 protein (S. cerevisiae) rn
YEAST_2:1774150_AT Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; activator of Fab1p Sc
Protein Similarities
Method ID Description E-Value
blast CAB00043 Hypothetical protein K04G2.6 [Caenorhabditis elegans] ref|NP_492215.1| K04G2.6 [Caenorhabditis elegans] 0.0
blast CAE63705 Hypothetical protein CBG08220 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:177699_AT
gtatcaactcttcatggcgttctactcactgccaccgaacttttcattttgagagatgaa
ctgcgtgctctctccaacgaatcttcccgatcattattcgaatgcattttccgtgtttgg
tcaaatcgtccgattgctcttttgggactgtgtctactgtcacaacattatcaacaagcg
gctgatattgcacttcttctttctcaagttgatatcacagttgatgtactactggagatt
gacaaacttgtgaaccttgtcgagagtcctgttctcgcttatgttcgtatggaccttctt
tcgtcaattcatcgccctccactatgcacagttttatcagctcttttgatgcttctccca
caatctgaagcattcgtcaccctacataaacgtcttcaggcagttccggcactagtgtct
attgataactctccgtatgctccaaaagtcccaccatgcagaatcgacttcccaccatta
atgctccatttcaaagcagctcttgctcgccgtcaaaccgaagttcgctcacgtcacagg
gatcttttatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATCAACTCTTCATGGCGTTCTAC 17 455 1510 Antisense
ACTGCCACCGAACTTTTCATTTTGA 172 97 1537 Antisense
TCCAACGAATCTTCCCGATCATTAT 582 587 1582 Antisense
GAACCTTGTCGAGAGTCCTGTTCTC 329 345 1761 Antisense
TCCTGTTCTCGCTTATGTTCGTATG 397 593 1776 Antisense
ATGGACCTTCTTTCGTCAATTCATC 5 53 1798 Antisense
GCATTCGTCACCCTACATAAACGTC 537 305 1879 Antisense
GCAGTTCCGGCACTAGTGTCTATTG 666 311 1909 Antisense
TAACTCTCCGTATGCTCCAAAAGTC 513 639 1935 Antisense
TCCAAAAGTCCCACCATGCAGAATC 492 587 1950 Antisense
GCTCACGTCACAGGGATCTTTTATC 463 301 2036 Antisense
Sequence Source Affymetrix Proprietary Database