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[177693_at]

Description R03G8.3 /REP_DB=WormBase Gene ID /WP=CE03527 /TR=Q21672 /GB=CAA93678.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 63      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 81      6grp_total > 63      4grp_total > 17      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 260
1 2 3 4 range
A 74.2
(-3)
P 		119.8
(-1.6)
P 		96
(-2.3)
P 		272.4
(3)
P 		198
B 56.2
(-3)
P 		130.5
(-0.6)
P 		132.4
(-0.5)
P 		242
(3)
P 		186
C 70.8
(-3)
P 		93.9
(-2.4)
P 		125.4
(-1.7)
P 		315.9
(3)
P 		245
avr 67.1
(-3) 		114.7
(-1.6) 		117.9
(-1.5) 		276.8
(3) 		210

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 267
1 2 3 4 5 6 range
A 126.1
(-3)
P 		253.9
(0.5)
P 		341.5
(2.9)
P 		343.8
(3)
P 		184.4
(-1.4)
P 		229.6
(-0.1)
P 		218
B 129.9
(-3)
P 		208.1
(-0.4)
P 		312.6
(3)
P 		200.2
(-0.7)
P 		287.6
(2.2)
P 		241.6
(0.7)
P 		183
C 137.5
(-3)
P 		241.1
(-0.6)
P 		392.7
(3)
P 		299.5
(0.8)
P 		216.4
(-1.1)
P 		291.8
(0.6)
P 		255
avr 131.2
(-3) 		234.4
(-0.2) 		348.9
(3) 		281.2
(1.1) 		229.5
(-0.3) 		254.3
(0.4) 		218
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 35
1 2 3 4 range
A 27
(-2.2)
P 		26
(-2.6)
P 		25.1
(-3)
P 		39.9
(3)
P 		15
B 17.9
(-3)
P 		32.2
(-0.5)
P 		30.7
(-0.7)
P 		51.6
(3)
P 		34
C 23.4
(-3)
P 		27
(-2.3)
P 		43.7
(1.1)
P 		53.3
(3)
P 		30
avr 22.8
(-3) 		28.4
(-1.7) 		33.2
(-0.6) 		48.3
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 23.2
(-3)
P 		90.3
(3)
P 		74.4
(1.6)
P 		87.2
(2.7)
P 		55.6
(-0.1)
P 		55.9
(-0.1)
P 		67
B 45.2
(-3)
P 		69.3
(-1)
P 		75.9
(-0.4)
P 		53
(-2.3)
P 		116.9
(3)
P 		90.1
(0.8)
P 		72
C 51
(-3)
P 		73.6
(-0.2)
P 		100
(3)
P 		60.2
(-1.9)
P 		70
(-0.7)
P 		97.7
(2.7)
P 		49
avr 39.8
(-3) 		77.7
(2.2) 		83.4
(3) 		66.8
(0.7) 		80.8
(2.6) 		81.2
(2.7) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177693_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17779
Sequence Type Exemplar sequence
Representative Public ID R03G8.3 NCBI
Target Description R03G8.3 /REP_DB=WormBase Gene ID /WP=CE03527 /TR=Q21672 /GB=CAA93678.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077773(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077773 NCBI Caenorhabditis elegans R03G8.3 (R03G8.3) mRNA, complete cds. 11/11 None
SNAP00000031813 ENSEMBL cdna:SNAP chromosome:CEL140:X:13091051:13094146:1 11/11 None
GENEFINDER00000031818 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:13091072:13094146:1 11/11 None
R03G8.3 ENSEMBL cdna:known chromosome:CEL140:X:13090278:13094344:1 gene:R03G8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13090277-13094146(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R03G8.3
Entrez Gene 181437 Entrez gene
SwissProt Q21672 EMBL-EBI
WormBase CE37395 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA93678 Hypothetical protein R03G8.3 [Caenorhabditis elegans] ref|NP_510174.2| R03G8.3 [Caenorhabditis elegans] 0.0
blast CAE61382 Hypothetical protein CBG05229 [Caenorhabditis briggsae] 0.0
blast CAB00864 Hypothetical protein K03A11.4 [Caenorhabditis elegans] ref|NP_510170.2| K03A11.4 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004245 EMBL-EBI Protein of unknown function DUF229 1.5E-118
Pfam IPR004245 EMBL-EBI Protein of unknown function DUF229 1.5E-118
Pfam IPR004245 EMBL-EBI Protein of unknown function DUF229 1.5E-118
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510174.2 1 116-138
R03G8.3 1 116-138
SNAP00000031813 1 13-35
GENEFINDER00000031818 1 9-31
Sequence
Target Sequence 
>C. ELEGANS:177693_AT
caatctgcaagcaacttcaccgacctcgaccctctgataattcccggcgagaaaggatat
tcgtatatccggacacagccggatattttgagaaactgccgaaacatgccaattcctatg
caatattgcatttgtcagttcaataaaacacaagtttccacaaaaagtgaaacagctaag
ggggttggcgaagctgtggctctagctgtgaataatgaaattgaagaaggcaaattcacg
gacaaatgtgttaaaatgaaagttgatcacgttgtctcacttttgcagtttgaccaaaag
tttaaaggttccatgttatacacggcggtcgttgaaatgacaaaaccaagtaaagcagtt
tataaggcaaatgtgaaaattctcgaaaacggagaagttaaagtgttaggaatggtagag
aggagtaacaaatatggaagcactgcatactgcataggatcagagcatcatcggccatac
tgttattgcaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATCTGCAAGCAACTTCACCGACC 62 183 1741 Antisense
CCCTCTGATAATTCCCGGCGAGAAA 560 259 1770 Antisense
ATATTCGTATATCCGGACACAGCCG 377 19 1797 Antisense
AACTGCCGAAACATGCCAATTCCTA 172 141 1834 Antisense
TAAGGGGGTTGGCGAAGCTGTGGCT 222 249 1917 Antisense
AGTTGATCACGTTGTCTCACTTTTG 375 65 2001 Antisense
GTTGTCTCACTTTTGCAGTTTGACC 604 437 2011 Antisense
AGGTTCCATGTTATACACGGCGGTC 569 1 2046 Antisense
GAAGCACTGCATACTGCATAGGATC 157 337 2177 Antisense
AGGATCAGAGCATCATCGGCCATAC 459 57 2196 Antisense
TCATCGGCCATACTGTTATTGCAAG 204 621 2208 Antisense
Sequence Source Affymetrix Proprietary Database