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[177686_at]

Description F33A8.2 /REP_DB=WormBase Gene ID /WP=CE17754 /TR=O62211 /GB=CAB04255.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 21      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 46      6grp_total > 32      4grp_total > 14      6grp_highest_sub > De      6grp_cgc > 70      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3863
1 2 3 4 range
A 4124.3
(2.2)
P 		1844.2
(-2.8)
P 		1743.8
(-3)
P 		4504.6
(3)
P 		2761
B 4073.9
(1.4)
P 		1873.2
(-2.1)
P 		1272.8
(-3)
P 		5135.6
(3)
P 		3863
C 4135.2
(1.4)
P 		2055.1
(-2.4)
P 		1742.8
(-3)
P 		4976
(3)
P 		3233
avr 4111.1
(1.6) 		1924.2
(-2.4) 		1586.5
(-3) 		4872.1
(3) 		3286

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1664
1 2 3 4 5 6 range
A 3083.5
(-0.1)
P 		3033.4
(-0.3)
P 		3811.1
(3)
P 		3014.4
(-0.4)
P 		2765.3
(-1.4)
P 		2390.5
(-3)
P 		1421
B 3228.8
(2.6)
P 		3246.7
(2.7)
P 		3298.4
(3)
P 		3237.5
(2.7)
P 		2991.8
(1.4)
P 		2147.6
(-3)
P 		1151
C 3592.1
(3)
P 		3185.8
(0.8)
P 		3423.4
(2.1)
P 		2948.1
(-0.5)
P 		2788.9
(-1.4)
P 		2490.7
(-3)
P 		1101
avr 3301.5
(1.9) 		3155.3
(1.2) 		3511
(3) 		3066.7
(0.7) 		2848.7
(-0.4) 		2342.9
(-3) 		1168
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1207
1 2 3 4 range
A 1501.6
(3)
P 		400.3
(-3)
P 		455.9
(-2.7)
P 		659.4
(-1.6)
P 		1101
B 1299.1
(3)
P 		462.6
(-2)
P 		294.9
(-3)
P 		1094.4
(1.8)
P 		1004
C 1368.9
(3)
P 		590.9
(-3)
P 		607.4
(-2.9)
P 		839
(-1.1)
P 		778
avr 1389.9
(3) 		484.6
(-2.8) 		452.7
(-3) 		864.3
(-0.4) 		937

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 763
1 2 3 4 5 6 range
A 568.4
(-3)
P 		1078.8
(3)
P 		830
(0.1)
P 		764.5
(-0.7)
P 		834.4
(0.1)
P 		582
(-2.8)
P 		510
B 1123.5
(1.7)
P 		1080.7
(1)
P 		800.9
(-3)
P 		857.5
(-2.2)
P 		1215.5
(3)
P 		800.8
(-3)
P 		415
C 1331.4
(3)
P 		972.1
(0.1)
P 		871.3
(-0.7)
P 		592.9
(-3)
P 		902.5
(-0.5)
P 		833.8
(-1)
P 		739
avr 1007.8
(2.3) 		1043.9
(3) 		834.1
(-1.1) 		738.3
(-3) 		984.1
(1.8) 		738.9
(-3) 		306
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177686_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3119
Sequence Type Exemplar sequence
Representative Public ID F33A8.2 NCBI
Target Description F33A8.2 /REP_DB=WormBase Gene ID /WP=CE17754 /TR=O62211 /GB=CAB04255.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063964(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063964 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-18) (nlp-18) mRNA, complete cds. 10/11 None
SNAP00000001850 ENSEMBL cdna:SNAP chromosome:CEL140:II:11028088:11030286:-1 10/11 None
GENEFINDER00000001880 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11028088:11030286:-1 9/11 None
F33A8.2.3 ENSEMBL cdna:known chromosome:CEL140:II:11027874:11030309:-1 gene:F33A8.2 10/11 None
F33A8.2.2 ENSEMBL cdna:known chromosome:CEL140:II:11027878:11030316:-1 gene:F33A8.2 10/11 None
F33A8.2.1 ENSEMBL cdna:known chromosome:CEL140:II:11027891:11030316:-1 gene:F33A8.2 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11028084-11030283(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-18
Ensembl F33A8.2
Entrez Gene 185219 Entrez gene
SwissProt O62211 EMBL-EBI
WormBase CE32885 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04255 Hypothetical protein F33A8.2 [Caenorhabditis elegans] ref|NP_496365.2| Neuropeptide-Like Protein family member (nlp-18) [Caenorhabditis elegans] 2.0E-50
blast CAE59583 Hypothetical protein CBG02983 [Caenorhabditis briggsae] 1.0E-43
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496365.2 1 5-24
F33A8.2.3 1 5-24
F33A8.2.2 1 5-24
F33A8.2.1 1 5-24
SNAP00000001850 1 5-24
GENEFINDER00000001880 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:177686_AT
tgaacgccaacgtctactcgattgtctacttcctctcgttcctcgttctctgcatttcag
cacaattggtaagtgagtcagttcagaaagtgaaaacaagtatggaacggggagcgtcta
gaaaagcggaatcatttgattctgacaaaggggctcaacacgctgacagcggagccactg
aggtggatggaatcgtcgacaaacgctcaccatacagggcattcgcattcgcaaaacgaa
gtgacgaagagaacctcgacttcctcgagaagcgtgcgagatacggatttgccaagagat
ctccataccgaacatttgccttcgccaaacgtgcctctccatacggattcgcatttgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAACGCCAACGTCTACTCGATTGT 130 573 14 Antisense
CTCGTTCTCTGCATTTCAGCACAAT 448 233 55 Antisense
TGACAAAGGGGCTCAACACGCTGAC 132 571 156 Antisense
CTGACAGCGGAGCCACTGAGGTGGA 173 237 176 Antisense
TGGAATCGTCGACAAACGCTCACCA 563 545 201 Antisense
CAAACGCTCACCATACAGGGCATTC 105 193 213 Antisense
ACAGGGCATTCGCATTCGCAAAACG 549 95 227 Antisense
ACGAAGAGAACCTCGACTTCCTCGA 665 89 257 Antisense
TACGGATTTGCCAAGAGATCTCCAT 385 647 295 Antisense
AGATCTCCATACCGAACATTTGCCT 184 67 310 Antisense
CTCTCCATACGGATTCGCATTTGCC 563 229 348 Antisense
Sequence Source Affymetrix Proprietary Database